Characterization of retinol-binding protein in familial hypo-retinol-binding proteinemia

Toshihiko Matsuo, N. Matsuo

Research output: Contribution to journalArticle

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Abstract

We reported previously familial hypo-retinol-binding proteinemia in a child who developed keratomalacia during measles infection. In the present study, we characterized serum retinol-binding proteins (RBPs) in the affected family members and compared these RBPs with those in the unaffected family members. Immunoblotting following SDS-polyacrylamide gel electrophoresis and isoelectric focusing demonstrated no differences in molecular weight and isoelectric point between the RBPs from the affected and unaffected family members, as far as RBPs were detected by antibody against standard RBP. Gel filtration revealed that all the RBPs in the serum of the affected family members made a complex with prealbumin.

Original languageEnglish
Pages (from-to)379-384
Number of pages6
JournalJapanese Journal of Ophthalmology
Volume32
Issue number4
Publication statusPublished - 1988

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Retinol-Binding Proteins
Vitamin A
Prealbumin
Isoelectric Point
Measles
Isoelectric Focusing
Serum
Immunoblotting
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Molecular Weight
Antibodies
Infection

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Characterization of retinol-binding protein in familial hypo-retinol-binding proteinemia. / Matsuo, Toshihiko; Matsuo, N.

In: Japanese Journal of Ophthalmology, Vol. 32, No. 4, 1988, p. 379-384.

Research output: Contribution to journalArticle

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