Characterization of PDF-immunoreactive neurons in the optic lobe and cerebral lobe of the cricket, Gryllus bimaculatus

Salaheldin Abdelsalam, Hiroyuki Uemura, Yujiro Umezaki, A. S M Saifullah, Miki Shimohigashi, Kenji Tomioka

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Pigment-dispersing factor (PDF) is a neuropeptide playing important roles in insect circadian systems. In this study, we morphologically and physiologically characterized PDF-immunoreactive neurons in the optic lobe and the brain of the cricket Gryllus bimaculatus. PDF-immunoreactivity was detected in cells located in the proximal medulla (PDFMe cells) and those in the dorsal and ventral regions of the outer chiasma (PDFLa cells). The PDFMe cells had varicose processes spread over the frontal surface of the medulla and the PDFLa cells had varicose mesh-like innervations in almost whole lamina, suggesting their modulatory role in the optic lobe. Some of PDFMe cells had a hairpin-shaped axonal process running toward the lamina then turning back to project into the brain where they terminated at various protocerebral areas. The PDFMe cells had a low frequency spontaneous spike activity that was higher during the night and was often slightly increased by light pulses. Six pairs of PDF-immunoreactive neurons were also found in the frontal ganglion. Competitive ELISA with anti-PDF antibodies revealed daily cycling of PDF both in the optic lobe and cerebral lobe with an increase during the night that persisted in constant darkness. The physiological role of PDF is discussed based on these results.

Original languageEnglish
Pages (from-to)1205-1212
Number of pages8
JournalJournal of Insect Physiology
Volume54
Issue number7
DOIs
Publication statusPublished - Jul 2008

Fingerprint

optic lobe
Gryllidae
Gryllus bimaculatus
neurons
pigments
Neurons
cells
laminae (animals)
brain
chiasmata
Darkness
neuropeptides
Brain
innervation
Neuropeptides
Ganglia
Insects
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Light

Keywords

  • Circadian rhythm
  • Cricket
  • Optic lobe
  • PDF neuron
  • Pigment-dispersing factor

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

Characterization of PDF-immunoreactive neurons in the optic lobe and cerebral lobe of the cricket, Gryllus bimaculatus. / Abdelsalam, Salaheldin; Uemura, Hiroyuki; Umezaki, Yujiro; Saifullah, A. S M; Shimohigashi, Miki; Tomioka, Kenji.

In: Journal of Insect Physiology, Vol. 54, No. 7, 07.2008, p. 1205-1212.

Research output: Contribution to journalArticle

Abdelsalam, Salaheldin ; Uemura, Hiroyuki ; Umezaki, Yujiro ; Saifullah, A. S M ; Shimohigashi, Miki ; Tomioka, Kenji. / Characterization of PDF-immunoreactive neurons in the optic lobe and cerebral lobe of the cricket, Gryllus bimaculatus. In: Journal of Insect Physiology. 2008 ; Vol. 54, No. 7. pp. 1205-1212.
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