Characterization of NtChitIV, a class IV chitinase induced by β-1,3-, 1,6-glucan elicitor from Alternaria alternata 102: Antagonistic effect of salicylic acid and methyl jasmonate on the induction of NtChitIV

Tomonori Shinya, Kazunari Hanai, Ivan Gális, Kaoru Suzuki, Ken Matsuoka, Hideaki Matsuoka, Mikako Saito

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Abstract

Tobacco BY-2 class IV chitinases (TBC-1, TBC-3) were rapidly and transiently induced by the β-1,3-, 1,6-glucan elicitor from Alternaria alternata 102 (AaGlucan). The full-length cDNA and 5′-flanking region of a gene encoding class IV chitinases were isolated on the basis of the amino acid sequence of TBC-1. Sequence analysis indicated that NtChitIV encoded TBC-1, TBC-3, or both. Since purified TBC-1 and TBC-3 from BY-2 cells lack a chitin binding domain in the N-terminal region, these enzymes suggested to be derived from NtChitIV by post-translational proteolytic processing. The transcripts of NtChitIV accumulated rapidly within 1 h after treatment with AaGlucan. Accumulation was maximal 3 h after treatment. Reporter gene assays were used to analyze the promoter regions involved in the transcriptional control of NtChitIV, and these assays revealed that the 1.89-kb NtChitIV promoter was activated by AaGlucan but not by salicylic acid (SA) or methyl jasmonate (MeJA). The AaGlucan-induced transcriptional activation via 1.89-kb NtChitIV promoter was attenuated by pretreatment with SA or MeJA. These results suggest that NtChitIV expression is particularly induced by AaGlucan and that the AaGlucan-dependent signaling pathway is different from the SA- and MeJA-dependent signaling pathways.

Original languageEnglish
Pages (from-to)311-317
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume353
Issue number2
DOIs
Publication statusPublished - Feb 9 2007
Externally publishedYes

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Keywords

  • BY-2
  • Chitinase
  • Elicitor
  • Gene expression
  • Promoter
  • β-1,3-, 1,6-Glucan

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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