Characterization of mouse tissue kallikrein 5

Sanath Rajapakse, Katsueki Ogiwara, Noriko Yamano, Atsushi Kimura, Kensaku Hirata, Sumio Takahashi, Takayuki Takahashi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mouse tissue kallikreins (Klks) are members of a large, multigene family consisting of 37 genes, 26 of which can code for functional proteins. Mouse tissue kallikrein 5 (Klk5) has long been thought to be one of these functional genes, but the gene product, mK5, has not been isolated and characterized. In the present study, we prepared active recombinant mK5 using an Escherichia coli expression system, followed by column chromatography. We then determined the biochemical and enzymatic properties of purified mK5. mK5 had trypsin-like activity for Arg at the P1 position, and its activity was inhibited by typical serine protease inhibitors. mK5 degraded gelatin, fibronectin, collagen type IV, high-molecular-weight kininogen, and insulin-like growth factor binding protein-3. Our data suggest that mK5 may be implicated in the process of extracellular matrix remodeling.

Original languageEnglish
Pages (from-to)963-968
Number of pages6
JournalZoological Science
Volume23
Issue number11
DOIs
Publication statusPublished - Nov 2006

Fingerprint

tissue kallikrein
mice
cystatins
insulin-like growth factor binding proteins
genes
fibronectins
serine proteinases
proteinase inhibitors
gelatin
multigene family
extracellular matrix
trypsin
collagen
chromatography
molecular weight
Escherichia coli
proteins

Keywords

  • Characterization
  • Kallikrein 5
  • Mouse
  • Protease
  • Recombinant enzyme

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Rajapakse, S., Ogiwara, K., Yamano, N., Kimura, A., Hirata, K., Takahashi, S., & Takahashi, T. (2006). Characterization of mouse tissue kallikrein 5. Zoological Science, 23(11), 963-968. https://doi.org/10.2108/zsj.23.963

Characterization of mouse tissue kallikrein 5. / Rajapakse, Sanath; Ogiwara, Katsueki; Yamano, Noriko; Kimura, Atsushi; Hirata, Kensaku; Takahashi, Sumio; Takahashi, Takayuki.

In: Zoological Science, Vol. 23, No. 11, 11.2006, p. 963-968.

Research output: Contribution to journalArticle

Rajapakse, S, Ogiwara, K, Yamano, N, Kimura, A, Hirata, K, Takahashi, S & Takahashi, T 2006, 'Characterization of mouse tissue kallikrein 5', Zoological Science, vol. 23, no. 11, pp. 963-968. https://doi.org/10.2108/zsj.23.963
Rajapakse S, Ogiwara K, Yamano N, Kimura A, Hirata K, Takahashi S et al. Characterization of mouse tissue kallikrein 5. Zoological Science. 2006 Nov;23(11):963-968. https://doi.org/10.2108/zsj.23.963
Rajapakse, Sanath ; Ogiwara, Katsueki ; Yamano, Noriko ; Kimura, Atsushi ; Hirata, Kensaku ; Takahashi, Sumio ; Takahashi, Takayuki. / Characterization of mouse tissue kallikrein 5. In: Zoological Science. 2006 ; Vol. 23, No. 11. pp. 963-968.
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