Characterization of in vivo effects of platelet-rich plasma and biodegradable gelatin hydrogel microspheres on degenerated intervertebral discs

Kazuhide Sawamura, Takumi Ikeda, Masateru Nagae, Shin Ichi Okamoto, Yasuo Mikami, Hitoshi Hase, Kazuya Ikoma, Tetsuya Yamada, Hirotaka Sakamoto, Ken Ichi Matsuda, Yasuhiko Tabata, Mitsuhiro Kawata, Toshikazu Kubo

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

We have previously shown that administration of platelet-rich plasma-impregnated gelatin hydrogel microspheres (PRP-GHMs) into a degenerated intervertebral disc (IVD) markedly suppresses progression of IVD degeneration. In the current study, we characterized the in vivo effects of PRP-GHM treatment in a degenerated IVD model in rabbit. On magnetic resonance images, the IVD height was significantly greater after treatment with PRP-GHMs compared with phosphate-buffered saline-impregnated GHMs, PRP without GHMs, and needle puncture only. Water content was also preserved in PRP-GHM-treated IVDs. Consistent with this observation, the mRNA expression of proteoglycan core protein and type II collagen was significantly higher after PRP-GHM treatment compared with other treatment groups. No proliferating cells were found in the nucleus pulposus and inner annulus fibrosus in any groups, but the number of apoptotic cells in the nucleus pulposus after PRP-GHM treatment was significantly lower than that after other treatments. These results provide an improved understanding of the therapeutic effects of PRP-GHM treatment of degenerated IVDs.

Original languageEnglish
Pages (from-to)3719-3727
Number of pages9
JournalTissue Engineering - Part A
Volume15
Issue number12
DOIs
Publication statusPublished - Dec 1 2009
Externally publishedYes

Fingerprint

Platelet-Rich Plasma
Intervertebral Disc
Hydrogel
Gelatin
Platelets
Microspheres
Hydrogels
Plasmas
Therapeutics
Intervertebral Disc Degeneration
Collagen Type II
Therapeutic Uses
Proteoglycans
Magnetic resonance
Cell Nucleus
Punctures
Collagen
Needles
Water content
Phosphates

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials

Cite this

Characterization of in vivo effects of platelet-rich plasma and biodegradable gelatin hydrogel microspheres on degenerated intervertebral discs. / Sawamura, Kazuhide; Ikeda, Takumi; Nagae, Masateru; Okamoto, Shin Ichi; Mikami, Yasuo; Hase, Hitoshi; Ikoma, Kazuya; Yamada, Tetsuya; Sakamoto, Hirotaka; Matsuda, Ken Ichi; Tabata, Yasuhiko; Kawata, Mitsuhiro; Kubo, Toshikazu.

In: Tissue Engineering - Part A, Vol. 15, No. 12, 01.12.2009, p. 3719-3727.

Research output: Contribution to journalArticle

Sawamura, K, Ikeda, T, Nagae, M, Okamoto, SI, Mikami, Y, Hase, H, Ikoma, K, Yamada, T, Sakamoto, H, Matsuda, KI, Tabata, Y, Kawata, M & Kubo, T 2009, 'Characterization of in vivo effects of platelet-rich plasma and biodegradable gelatin hydrogel microspheres on degenerated intervertebral discs', Tissue Engineering - Part A, vol. 15, no. 12, pp. 3719-3727. https://doi.org/10.1089/ten.tea.2008.0697
Sawamura, Kazuhide ; Ikeda, Takumi ; Nagae, Masateru ; Okamoto, Shin Ichi ; Mikami, Yasuo ; Hase, Hitoshi ; Ikoma, Kazuya ; Yamada, Tetsuya ; Sakamoto, Hirotaka ; Matsuda, Ken Ichi ; Tabata, Yasuhiko ; Kawata, Mitsuhiro ; Kubo, Toshikazu. / Characterization of in vivo effects of platelet-rich plasma and biodegradable gelatin hydrogel microspheres on degenerated intervertebral discs. In: Tissue Engineering - Part A. 2009 ; Vol. 15, No. 12. pp. 3719-3727.
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