Characterization of IL-18 expression and release in the pathogenesis of chronic rhinosinusitis

Mitsuhiro Okano, Tazuko Fujiwara, Seiichiro Makihara, Rumi Fujiwara, Takaya Higaki, Shin Kariya, Yohei Noda, Takenori Haruna, Kazunori Nishizaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Interleukin-18 (IL-18) is a member of the IL-1 cytokine family that affects chronic inflammation. We sought to characterize IL-18 expression and investigate its release during chronic rhinosinusitis (CRS). Methods: The expression of IL-18 in nasal polyps (NPs) and uncinate tissues (UTs) from both CRS and non-CRS patients was examined via immunohistochemistry. After culturing dispersed NP cells (DNPCs) with or without various stimulations, IL-18 levels were measured in the culture supernatants. Furthermore, the effect of IL-18 neutralization on staphylococcus enterotoxin B (SEB)-induced cytokine production was also examined. Results: Similar expression of IL-18 in the epithelial layers was observed between the NPs and UTs. However, there was a significantly higher number of IL-18+ cells in the lamina propria from NPs compared to UTs without CRS. This increased number was significantly correlated with the radiological severity of sinusitis and local eosinophilia. After the dispersion, IL-18 was spontaneously released by NP cells in a phase-dependent manner. While SEB, fungal antigens, and TLR agonists did not enhance the release, exposure to protease or one cycle of a freeze-and-thaw treatment did induce release of IL-18 from rested DNPCs. In addition, neutralization of IL-18 significantly suppressed SEB-induced IL-5, IL-13, and IFN-γ, but not IL-17A production. Conclusions: These results suggest that the pro-inflammatory effect of IL-18 released by danger signals may be involved in the pathogenesis of CRS, which includes eosinophilic inflammation and NP formation, via the augmentation of both Th2- and Th1-associated cytokine production.

Original languageEnglish
Pages (from-to)275-286
Number of pages12
JournalInternational Archives of Allergy and Immunology
Volume160
Issue number3
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Interleukin-18
Nasal Polyps
Cytokines
Fungal Antigens
Inflammation
Interleukin-13
Interleukin-17
Sinusitis
Interleukin-5
Eosinophilia
Interleukin-1
Mucous Membrane
Peptide Hydrolases
Immunohistochemistry

Keywords

  • Eosinophilic inflammation
  • IFN-γ
  • Interleukin-13
  • Interleukin-18
  • Interleukin-5
  • Nasal polyps
  • Protease
  • Rhinosinusitis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Characterization of IL-18 expression and release in the pathogenesis of chronic rhinosinusitis. / Okano, Mitsuhiro; Fujiwara, Tazuko; Makihara, Seiichiro; Fujiwara, Rumi; Higaki, Takaya; Kariya, Shin; Noda, Yohei; Haruna, Takenori; Nishizaki, Kazunori.

In: International Archives of Allergy and Immunology, Vol. 160, No. 3, 02.2013, p. 275-286.

Research output: Contribution to journalArticle

Okano, Mitsuhiro ; Fujiwara, Tazuko ; Makihara, Seiichiro ; Fujiwara, Rumi ; Higaki, Takaya ; Kariya, Shin ; Noda, Yohei ; Haruna, Takenori ; Nishizaki, Kazunori. / Characterization of IL-18 expression and release in the pathogenesis of chronic rhinosinusitis. In: International Archives of Allergy and Immunology. 2013 ; Vol. 160, No. 3. pp. 275-286.
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abstract = "Background: Interleukin-18 (IL-18) is a member of the IL-1 cytokine family that affects chronic inflammation. We sought to characterize IL-18 expression and investigate its release during chronic rhinosinusitis (CRS). Methods: The expression of IL-18 in nasal polyps (NPs) and uncinate tissues (UTs) from both CRS and non-CRS patients was examined via immunohistochemistry. After culturing dispersed NP cells (DNPCs) with or without various stimulations, IL-18 levels were measured in the culture supernatants. Furthermore, the effect of IL-18 neutralization on staphylococcus enterotoxin B (SEB)-induced cytokine production was also examined. Results: Similar expression of IL-18 in the epithelial layers was observed between the NPs and UTs. However, there was a significantly higher number of IL-18+ cells in the lamina propria from NPs compared to UTs without CRS. This increased number was significantly correlated with the radiological severity of sinusitis and local eosinophilia. After the dispersion, IL-18 was spontaneously released by NP cells in a phase-dependent manner. While SEB, fungal antigens, and TLR agonists did not enhance the release, exposure to protease or one cycle of a freeze-and-thaw treatment did induce release of IL-18 from rested DNPCs. In addition, neutralization of IL-18 significantly suppressed SEB-induced IL-5, IL-13, and IFN-γ, but not IL-17A production. Conclusions: These results suggest that the pro-inflammatory effect of IL-18 released by danger signals may be involved in the pathogenesis of CRS, which includes eosinophilic inflammation and NP formation, via the augmentation of both Th2- and Th1-associated cytokine production.",
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T1 - Characterization of IL-18 expression and release in the pathogenesis of chronic rhinosinusitis

AU - Okano, Mitsuhiro

AU - Fujiwara, Tazuko

AU - Makihara, Seiichiro

AU - Fujiwara, Rumi

AU - Higaki, Takaya

AU - Kariya, Shin

AU - Noda, Yohei

AU - Haruna, Takenori

AU - Nishizaki, Kazunori

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N2 - Background: Interleukin-18 (IL-18) is a member of the IL-1 cytokine family that affects chronic inflammation. We sought to characterize IL-18 expression and investigate its release during chronic rhinosinusitis (CRS). Methods: The expression of IL-18 in nasal polyps (NPs) and uncinate tissues (UTs) from both CRS and non-CRS patients was examined via immunohistochemistry. After culturing dispersed NP cells (DNPCs) with or without various stimulations, IL-18 levels were measured in the culture supernatants. Furthermore, the effect of IL-18 neutralization on staphylococcus enterotoxin B (SEB)-induced cytokine production was also examined. Results: Similar expression of IL-18 in the epithelial layers was observed between the NPs and UTs. However, there was a significantly higher number of IL-18+ cells in the lamina propria from NPs compared to UTs without CRS. This increased number was significantly correlated with the radiological severity of sinusitis and local eosinophilia. After the dispersion, IL-18 was spontaneously released by NP cells in a phase-dependent manner. While SEB, fungal antigens, and TLR agonists did not enhance the release, exposure to protease or one cycle of a freeze-and-thaw treatment did induce release of IL-18 from rested DNPCs. In addition, neutralization of IL-18 significantly suppressed SEB-induced IL-5, IL-13, and IFN-γ, but not IL-17A production. Conclusions: These results suggest that the pro-inflammatory effect of IL-18 released by danger signals may be involved in the pathogenesis of CRS, which includes eosinophilic inflammation and NP formation, via the augmentation of both Th2- and Th1-associated cytokine production.

AB - Background: Interleukin-18 (IL-18) is a member of the IL-1 cytokine family that affects chronic inflammation. We sought to characterize IL-18 expression and investigate its release during chronic rhinosinusitis (CRS). Methods: The expression of IL-18 in nasal polyps (NPs) and uncinate tissues (UTs) from both CRS and non-CRS patients was examined via immunohistochemistry. After culturing dispersed NP cells (DNPCs) with or without various stimulations, IL-18 levels were measured in the culture supernatants. Furthermore, the effect of IL-18 neutralization on staphylococcus enterotoxin B (SEB)-induced cytokine production was also examined. Results: Similar expression of IL-18 in the epithelial layers was observed between the NPs and UTs. However, there was a significantly higher number of IL-18+ cells in the lamina propria from NPs compared to UTs without CRS. This increased number was significantly correlated with the radiological severity of sinusitis and local eosinophilia. After the dispersion, IL-18 was spontaneously released by NP cells in a phase-dependent manner. While SEB, fungal antigens, and TLR agonists did not enhance the release, exposure to protease or one cycle of a freeze-and-thaw treatment did induce release of IL-18 from rested DNPCs. In addition, neutralization of IL-18 significantly suppressed SEB-induced IL-5, IL-13, and IFN-γ, but not IL-17A production. Conclusions: These results suggest that the pro-inflammatory effect of IL-18 released by danger signals may be involved in the pathogenesis of CRS, which includes eosinophilic inflammation and NP formation, via the augmentation of both Th2- and Th1-associated cytokine production.

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KW - IFN-γ

KW - Interleukin-13

KW - Interleukin-18

KW - Interleukin-5

KW - Nasal polyps

KW - Protease

KW - Rhinosinusitis

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