Characterization of Human Autoantibodies Reactive to Gastric Parietal Cells

Xiao Hui Wang; Yoshiji Miyazaki; Yasuhisa Shinomura; Yoshinori Moriyama; Robert K. Nakamoto; Yuji Matsuzawa; Masatomo Maeda; Masamitsu Futai

doi:10.1006/bbrc.1993.1032

Characterization of Human Autoantibodies Reactive to Gastric Parietal Cells

Xiao Hui Wang, Yoshiji Miyazaki, Yasuhisa Shinomura, Yoshinori Moriyama, Robert K. Nakamoto, Yuji Matsuzawa, Masatomo Maeda, Masamitsu Futai

Faculty of Pharmaceutical Sciences

Research output: Contribution to journal › Article

4 Citations (Scopus)

Abstract

Human sera from autoimmune gastritis patients containing autoantibodies to gastric parietal cells were analyzed by immunological methods. Enzyme linked immuno-sorbent assay demonstrated that all nine sera reacted with pig gastric vesicles enriched in H⁺/K⁺-ATPase (gastric proton pump). Immunoblotting experiments indicated that the a subunit of the H⁺/K⁺-ATPase was the major antigen in the vesicles with two of the sera reacting strongly. We further characterized the specificitiy of the antibodies using partial sequences of the pig α subunit fused with truncated TrpE (anthranilate synthase). The antibodies from autoimmune gastritis patients reacted differently to the two fusion proteins (Met-1 to Ala-79, and Arg-606 to Ile-964), indicating that each patient sera contains a mixture of autoantibodies recognizing different epitopes with variable contents.

Original language	English
Pages (from-to)	207-214
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	190
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1993.1032
Publication status	Published - Jan 1 1993

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Wang, X. H., Miyazaki, Y., Shinomura, Y., Moriyama, Y., Nakamoto, R. K., Matsuzawa, Y., Maeda, M., & Futai, M. (1993). Characterization of Human Autoantibodies Reactive to Gastric Parietal Cells. Biochemical and Biophysical Research Communications, 190(1), 207-214. https://doi.org/10.1006/bbrc.1993.1032

Characterization of Human Autoantibodies Reactive to Gastric Parietal Cells. / Wang, Xiao Hui; Miyazaki, Yoshiji; Shinomura, Yasuhisa; Moriyama, Yoshinori; Nakamoto, Robert K.; Matsuzawa, Yuji; Maeda, Masatomo; Futai, Masamitsu.

In: Biochemical and Biophysical Research Communications, Vol. 190, No. 1, 01.01.1993, p. 207-214.

Research output: Contribution to journal › Article

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abstract = "Human sera from autoimmune gastritis patients containing autoantibodies to gastric parietal cells were analyzed by immunological methods. Enzyme linked immuno-sorbent assay demonstrated that all nine sera reacted with pig gastric vesicles enriched in H+/K+-ATPase (gastric proton pump). Immunoblotting experiments indicated that the a subunit of the H+/K+-ATPase was the major antigen in the vesicles with two of the sera reacting strongly. We further characterized the specificitiy of the antibodies using partial sequences of the pig α subunit fused with truncated TrpE (anthranilate synthase). The antibodies from autoimmune gastritis patients reacted differently to the two fusion proteins (Met-1 to Ala-79, and Arg-606 to Ile-964), indicating that each patient sera contains a mixture of autoantibodies recognizing different epitopes with variable contents.",

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