Characterization of Human Autoantibodies Reactive to Gastric Parietal Cells

Xiao Hui Wang, Yoshiji Miyazaki, Yasuhisa Shinomura, Yoshinori Moriyama, Robert K. Nakamoto, Yuji Matsuzawa, Masatomo Maeda, Masamitsu Futai

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Human sera from autoimmune gastritis patients containing autoantibodies to gastric parietal cells were analyzed by immunological methods. Enzyme linked immuno-sorbent assay demonstrated that all nine sera reacted with pig gastric vesicles enriched in H+/K+-ATPase (gastric proton pump). Immunoblotting experiments indicated that the a subunit of the H+/K+-ATPase was the major antigen in the vesicles with two of the sera reacting strongly. We further characterized the specificitiy of the antibodies using partial sequences of the pig α subunit fused with truncated TrpE (anthranilate synthase). The antibodies from autoimmune gastritis patients reacted differently to the two fusion proteins (Met-1 to Ala-79, and Arg-606 to Ile-964), indicating that each patient sera contains a mixture of autoantibodies recognizing different epitopes with variable contents.

Original languageEnglish
Pages (from-to)207-214
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume190
Issue number1
DOIs
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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