Characterization of Human Autoantibodies Reactive to Gastric Parietal Cells

X. H. Wang, Y. Miyazaki, Y. Shinomura, Y. Moriyama, R. K. Nakamoto, Y. Matsuzawa, M. Maeda, M. Futai

Research output: Contribution to journalArticle

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Abstract

Human sera from autoimmune gastritis patients containing autoantibodies to gastric parietal cells were analyzed by immunological methods. Enzyme linked immuno-sorbent assay demonstrated that all nine sera reacted with pig gastric vesicles enriched in H+/K+-ATPase (gastric proton pump). Immunoblotting experiments indicated that the a subunit of the H+/K+-ATPase was the major antigen in the vesicles with two of the sera reacting strongly. We further characterized the specificitiy of the antibodies using partial sequences of the pig α subunit fused with truncated TrpE (anthranilate synthase). The antibodies from autoimmune gastritis patients reacted differently to the two fusion proteins (Met-1 to Ala-79, and Arg-606 to Ile-964), indicating that each patient sera contains a mixture of autoantibodies recognizing different epitopes with variable contents.

Original languageEnglish
Pages (from-to)207-214
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume190
Issue number1
DOIs
Publication statusPublished - Jan 15 1993
Externally publishedYes

Fingerprint

Gastric Parietal Cells
Autoantibodies
Anthranilate Synthase
H(+)-K(+)-Exchanging ATPase
Proton Pumps
Antibodies
Gastritis
Sorbents
Serum
Adenosine Triphosphatases
Epitopes
Assays
Swine
Fusion reactions
Antigens
Proton-Translocating ATPases
Enzymes
Immunoblotting
Stomach
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Wang, X. H., Miyazaki, Y., Shinomura, Y., Moriyama, Y., Nakamoto, R. K., Matsuzawa, Y., ... Futai, M. (1993). Characterization of Human Autoantibodies Reactive to Gastric Parietal Cells. Biochemical and Biophysical Research Communications, 190(1), 207-214. https://doi.org/10.1006/bbrc.1993.1032

Characterization of Human Autoantibodies Reactive to Gastric Parietal Cells. / Wang, X. H.; Miyazaki, Y.; Shinomura, Y.; Moriyama, Y.; Nakamoto, R. K.; Matsuzawa, Y.; Maeda, M.; Futai, M.

In: Biochemical and Biophysical Research Communications, Vol. 190, No. 1, 15.01.1993, p. 207-214.

Research output: Contribution to journalArticle

Wang, XH, Miyazaki, Y, Shinomura, Y, Moriyama, Y, Nakamoto, RK, Matsuzawa, Y, Maeda, M & Futai, M 1993, 'Characterization of Human Autoantibodies Reactive to Gastric Parietal Cells', Biochemical and Biophysical Research Communications, vol. 190, no. 1, pp. 207-214. https://doi.org/10.1006/bbrc.1993.1032
Wang XH, Miyazaki Y, Shinomura Y, Moriyama Y, Nakamoto RK, Matsuzawa Y et al. Characterization of Human Autoantibodies Reactive to Gastric Parietal Cells. Biochemical and Biophysical Research Communications. 1993 Jan 15;190(1):207-214. https://doi.org/10.1006/bbrc.1993.1032
Wang, X. H. ; Miyazaki, Y. ; Shinomura, Y. ; Moriyama, Y. ; Nakamoto, R. K. ; Matsuzawa, Y. ; Maeda, M. ; Futai, M. / Characterization of Human Autoantibodies Reactive to Gastric Parietal Cells. In: Biochemical and Biophysical Research Communications. 1993 ; Vol. 190, No. 1. pp. 207-214.
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