Abstract
Human sera from autoimmune gastritis patients containing autoantibodies to gastric parietal cells were analyzed by immunological methods. Enzyme linked immuno-sorbent assay demonstrated that all nine sera reacted with pig gastric vesicles enriched in H+/K+-ATPase (gastric proton pump). Immunoblotting experiments indicated that the a subunit of the H+/K+-ATPase was the major antigen in the vesicles with two of the sera reacting strongly. We further characterized the specificitiy of the antibodies using partial sequences of the pig α subunit fused with truncated TrpE (anthranilate synthase). The antibodies from autoimmune gastritis patients reacted differently to the two fusion proteins (Met-1 to Ala-79, and Arg-606 to Ile-964), indicating that each patient sera contains a mixture of autoantibodies recognizing different epitopes with variable contents.
Original language | English |
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Pages (from-to) | 207-214 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 190 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1993 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology