Abstract
Human sera from autoimmune gastritis patients containing autoantibodies to gastric parietal cells were analyzed by immunological methods. Enzyme linked immuno-sorbent assay demonstrated that all nine sera reacted with pig gastric vesicles enriched in H+/K+-ATPase (gastric proton pump). Immunoblotting experiments indicated that the a subunit of the H+/K+-ATPase was the major antigen in the vesicles with two of the sera reacting strongly. We further characterized the specificitiy of the antibodies using partial sequences of the pig α subunit fused with truncated TrpE (anthranilate synthase). The antibodies from autoimmune gastritis patients reacted differently to the two fusion proteins (Met-1 to Ala-79, and Arg-606 to Ile-964), indicating that each patient sera contains a mixture of autoantibodies recognizing different epitopes with variable contents.
| Original language | English |
|---|---|
| Pages (from-to) | 207-214 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 190 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 15 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
Cite this
Characterization of Human Autoantibodies Reactive to Gastric Parietal Cells. / Wang, X. H.; Miyazaki, Y.; Shinomura, Y.; Moriyama, Y.; Nakamoto, R. K.; Matsuzawa, Y.; Maeda, M.; Futai, M.
In: Biochemical and Biophysical Research Communications, Vol. 190, No. 1, 15.01.1993, p. 207-214.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of Human Autoantibodies Reactive to Gastric Parietal Cells
AU - Wang, X. H.
AU - Miyazaki, Y.
AU - Shinomura, Y.
AU - Moriyama, Y.
AU - Nakamoto, R. K.
AU - Matsuzawa, Y.
AU - Maeda, M.
AU - Futai, M.
PY - 1993/1/15
Y1 - 1993/1/15
N2 - Human sera from autoimmune gastritis patients containing autoantibodies to gastric parietal cells were analyzed by immunological methods. Enzyme linked immuno-sorbent assay demonstrated that all nine sera reacted with pig gastric vesicles enriched in H+/K+-ATPase (gastric proton pump). Immunoblotting experiments indicated that the a subunit of the H+/K+-ATPase was the major antigen in the vesicles with two of the sera reacting strongly. We further characterized the specificitiy of the antibodies using partial sequences of the pig α subunit fused with truncated TrpE (anthranilate synthase). The antibodies from autoimmune gastritis patients reacted differently to the two fusion proteins (Met-1 to Ala-79, and Arg-606 to Ile-964), indicating that each patient sera contains a mixture of autoantibodies recognizing different epitopes with variable contents.
AB - Human sera from autoimmune gastritis patients containing autoantibodies to gastric parietal cells were analyzed by immunological methods. Enzyme linked immuno-sorbent assay demonstrated that all nine sera reacted with pig gastric vesicles enriched in H+/K+-ATPase (gastric proton pump). Immunoblotting experiments indicated that the a subunit of the H+/K+-ATPase was the major antigen in the vesicles with two of the sera reacting strongly. We further characterized the specificitiy of the antibodies using partial sequences of the pig α subunit fused with truncated TrpE (anthranilate synthase). The antibodies from autoimmune gastritis patients reacted differently to the two fusion proteins (Met-1 to Ala-79, and Arg-606 to Ile-964), indicating that each patient sera contains a mixture of autoantibodies recognizing different epitopes with variable contents.
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UR - http://www.scopus.com/inward/citedby.url?scp=0027248675&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1993.1032
DO - 10.1006/bbrc.1993.1032
M3 - Article
VL - 190
SP - 207
EP - 214
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -