Characterization of heat-induced interaction of neutral liposome with lipid membrane of Streptomyces griseus cell

Kien Xuan Ngo, Hiroshi Umakoshi, Toshinori Shimanouchi, Ryoichi Kuboi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The interaction between the neutral 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes and cell membrane of Streptomyces griseus induced by the heat treatment at specific temperature was investigated, focusing on the internalization of the neutral POPC liposomes with S. griseus cells. In an attempt to clarify the modes of liposome internalization, various kinds of inhibitors of endocytotic pathways were used to treat S. griseus cells. The efficiency of the heat treatment on liposome-cell membrane interactions was finally characterized based on the hydrophobic, electrostatic interactions and hydration effect. In fact, the internalization of the neutral liposomes into these cells was found to show higher rate and greater amount at higher temperatures. The kinetic study showed that the maximum amount of the internalized liposomes was, respectively, 469 × 105 and 643 × 105 liposomes/cell at 37 and 41 °C. The internalization of the neutral liposomes induced by the heat treatment was characterized, implying that the endocytosis occurred. The interactions involving the internalization, adsorption, and fusion of these liposomes with S. griseus cells were mainly contributed by the hydrophobic interaction and the unstable hydrogen bonds caused by the loss of water of surface hydration of cell membrane rather than the electrostatic interaction under the specific heat condition.

Original languageEnglish
Pages (from-to)399-407
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume73
Issue number2
DOIs
Publication statusPublished - Oct 15 2009
Externally publishedYes

Fingerprint

Streptomyces griseus
Liposomes
Membrane Lipids
lipids
Hot Temperature
membranes
heat
cells
heat treatment
interactions
Cell membranes
hydration
Heat treatment
Cell Membrane
electrostatics
Coulomb interactions
Static Electricity
Hydrophobic and Hydrophilic Interactions
Hydration
inhibitors

Keywords

  • Heat stress
  • Internalization
  • Membrane interaction
  • Neutral liposomes
  • Streptomyces griseus

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Characterization of heat-induced interaction of neutral liposome with lipid membrane of Streptomyces griseus cell. / Ngo, Kien Xuan; Umakoshi, Hiroshi; Shimanouchi, Toshinori; Kuboi, Ryoichi.

In: Colloids and Surfaces B: Biointerfaces, Vol. 73, No. 2, 15.10.2009, p. 399-407.

Research output: Contribution to journalArticle

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