Characterization of green fluorescent protein using aqueous two-phase systems

Hiroshi Umakoshi, Masato Nishida, Keishi Suga, Huong Thi Bui, Toshinori Shimanouchi, Ryoichi Kuboi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The surface net hydrophobicity and local hydrophobicity of green fluorescent protein was evaluated by using the aqueous two-phase partitioning method. The surface net hydrophobicity of GFP allowed it to be classified as a hydrophobic protein although the local hydrophobicity was low. The local hydrophobicity of the GFP was increased by a pH shift to acidic conditions and was maximal at pH 4. The surface net hydrophobicity increased at acidic conditions less than pH 4. The conformational change of GFP at acidic pH is discussed based on the above results.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalSolvent Extraction Research and Development
Volume16
Publication statusPublished - 2009
Externally publishedYes

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Hydrophobicity
Green Fluorescent Proteins
Proteins

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Characterization of green fluorescent protein using aqueous two-phase systems. / Umakoshi, Hiroshi; Nishida, Masato; Suga, Keishi; Bui, Huong Thi; Shimanouchi, Toshinori; Kuboi, Ryoichi.

In: Solvent Extraction Research and Development, Vol. 16, 2009, p. 145-150.

Research output: Contribution to journalArticle

Umakoshi, Hiroshi ; Nishida, Masato ; Suga, Keishi ; Bui, Huong Thi ; Shimanouchi, Toshinori ; Kuboi, Ryoichi. / Characterization of green fluorescent protein using aqueous two-phase systems. In: Solvent Extraction Research and Development. 2009 ; Vol. 16. pp. 145-150.
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