Two different types of gamma-glutamyltranspeptidase (γ-GTP) have been found in normal human pancreas following bromelain treatment. On the other hand, three human pancreatic ductal cell carcinomas have only a single type of γ-GTP upon analysis with polyacrylamide gel electrophoresis, anion-exchange column chromatography and isoelectric focusing. Carcinoma γ-GTPs were almost identical to one of the two types of normal pancreatic γ-GTPs. The γ-GTP from pancreatic carcinomas bound to anion-exchange column and was eluted at the same NaCl fractions as normal pancreatic γ-GTP. The properties of pancreatic carcinoma γ-GTP, as assessed by binding to concanavalin A and lentil lectin affinity columns, were also similar to one of the two enzymes of normal pancreas. No apparent difference in isoelectric points was found between the carcinoma γ-GTPs and one of the two normal pancreatic γ-GTPs.
- Anion-exchange column (Mono Q)
- Pancreatic carcinoma
- gamma-Glutamyltranspeptidase (γ-GTP)
- γ-GTP isoenzymes
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical