Characterization of gamma-glutamyltranspeptidase from human exocrine pancreatic carcinomas

Nozomi Yamaguchi, Manabu Sugimoto, Keiichi Kawai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Two different types of gamma-glutamyltranspeptidase (γ-GTP) have been found in normal human pancreas following bromelain treatment. On the other hand, three human pancreatic ductal cell carcinomas have only a single type of γ-GTP upon analysis with polyacrylamide gel electrophoresis, anion-exchange column chromatography and isoelectric focusing. Carcinoma γ-GTPs were almost identical to one of the two types of normal pancreatic γ-GTPs. The γ-GTP from pancreatic carcinomas bound to anion-exchange column and was eluted at the same NaCl fractions as normal pancreatic γ-GTP. The properties of pancreatic carcinoma γ-GTP, as assessed by binding to concanavalin A and lentil lectin affinity columns, were also similar to one of the two enzymes of normal pancreas. No apparent difference in isoelectric points was found between the carcinoma γ-GTPs and one of the two normal pancreatic γ-GTPs.

Original languageEnglish
Pages (from-to)47-54
Number of pages8
JournalClinica Chimica Acta
Volume149
Issue number1
DOIs
Publication statusPublished - Jun 30 1985
Externally publishedYes

Keywords

  • Anion-exchange column (Mono Q)
  • Pancreatic carcinoma
  • gamma-Glutamyltranspeptidase (γ-GTP)
  • γ-GTP isoenzymes

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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