Characterization of four plasma membrane aquaporins in tulip petals

A putative homolog is regulated by phosphorylation

Abul Kalam Azad, Maki Katsuhara, Yoshihiro Sawa, Takahiro Ishikawa, Hitoshi Shibata

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

We suggested previously that temperature-dependent tulip (Tulipa gesneriana) petal movement that is concomitant with water transport is regulated by reversible phosphorylation of an unidentified plasma membrane intrinsic protein (PIP). In this study, four full-length cDNAs of PIPs from tulip petals were identified and cloned. Two PIPs, namely TgPIP1;1 and TgPIP1;2, are members of the PIP1 subfamily, and the remaining two PIPs, namely TgPIP2;1 and TgPIP2;2, belong to the PIP2 subfamily of aquaporins and were named according to the nomenclature of PIP genes in plants. Of these four homologs, only TgPIP2;2 displayed significant water channel activity in the heterologous expression assay using Xenopus laevis oocytes. The water channel activity of this functional isoform was abolished by mercury and was affected by inhibitors of protein kinase and protein phosphatase. Using a site-directed mutagenesis approach to substitute several serine residues with alanine, and assessing water channel activity using the methylotrophic yeast Pichia pastoris expression assay, we showed that Ser35, Ser116 and Ser274 are the putative phosphorylation sites of TgPIP2;2. Real-time reverse transcription-PCR analysis revealed that the transcript levels of TgPIP1;1 and TgPIP1;2 in tulip petals, stems, leaves, bulbs and roots are very low when compared with those of TgPIP2;1 and TgPIP2;2. The transcript level of TgPIP2;1 is negligible in roots, and TgPIP2;2 is ubiquitously expressed in all organs with significant transcript levels. From the data reported herein, we suggest that TgPIP2;2 might be modulated by phosphorylation and dephosphorylation for regulating water channel activity, and may play a role in transcellular water transport in all tulip organs.

Original languageEnglish
Pages (from-to)1196-1208
Number of pages13
JournalPlant and Cell Physiology
Volume49
Issue number8
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Tulipa
Tulipa gesneriana
Aquaporins
aquaporins
plant-incorporated protectants
corolla
phosphorylation
plasma membrane
Phosphorylation
Cell Membrane
water
Blood Proteins
Membrane Proteins
membrane proteins
blood proteins
Transcytosis
Plant Genes
Pichia
Water
Phosphoprotein Phosphatases

Keywords

  • Aquaporin
  • Phosphorylation
  • PIP
  • Tulip
  • Water channel activity

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Characterization of four plasma membrane aquaporins in tulip petals : A putative homolog is regulated by phosphorylation. / Azad, Abul Kalam; Katsuhara, Maki; Sawa, Yoshihiro; Ishikawa, Takahiro; Shibata, Hitoshi.

In: Plant and Cell Physiology, Vol. 49, No. 8, 08.2008, p. 1196-1208.

Research output: Contribution to journalArticle

Azad, Abul Kalam ; Katsuhara, Maki ; Sawa, Yoshihiro ; Ishikawa, Takahiro ; Shibata, Hitoshi. / Characterization of four plasma membrane aquaporins in tulip petals : A putative homolog is regulated by phosphorylation. In: Plant and Cell Physiology. 2008 ; Vol. 49, No. 8. pp. 1196-1208.
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AB - We suggested previously that temperature-dependent tulip (Tulipa gesneriana) petal movement that is concomitant with water transport is regulated by reversible phosphorylation of an unidentified plasma membrane intrinsic protein (PIP). In this study, four full-length cDNAs of PIPs from tulip petals were identified and cloned. Two PIPs, namely TgPIP1;1 and TgPIP1;2, are members of the PIP1 subfamily, and the remaining two PIPs, namely TgPIP2;1 and TgPIP2;2, belong to the PIP2 subfamily of aquaporins and were named according to the nomenclature of PIP genes in plants. Of these four homologs, only TgPIP2;2 displayed significant water channel activity in the heterologous expression assay using Xenopus laevis oocytes. The water channel activity of this functional isoform was abolished by mercury and was affected by inhibitors of protein kinase and protein phosphatase. Using a site-directed mutagenesis approach to substitute several serine residues with alanine, and assessing water channel activity using the methylotrophic yeast Pichia pastoris expression assay, we showed that Ser35, Ser116 and Ser274 are the putative phosphorylation sites of TgPIP2;2. Real-time reverse transcription-PCR analysis revealed that the transcript levels of TgPIP1;1 and TgPIP1;2 in tulip petals, stems, leaves, bulbs and roots are very low when compared with those of TgPIP2;1 and TgPIP2;2. The transcript level of TgPIP2;1 is negligible in roots, and TgPIP2;2 is ubiquitously expressed in all organs with significant transcript levels. From the data reported herein, we suggest that TgPIP2;2 might be modulated by phosphorylation and dephosphorylation for regulating water channel activity, and may play a role in transcellular water transport in all tulip organs.

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