Characterization of flat revertants isolated from cells transformed by Abelson murine leukemia virus (Ab-MuLV)

Takashi Oka, Masuo Yutsudo, Hirokazu Inoue, Fujito Higuchi, Akira Hakura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Transformed Fisher rat fibroblast cell lines by Abelson murine leukemia virus frequently revert to the normal phenotype in usual culture conditions. Molecular biological analysis of three revertant clones isolated from the transformants showed that their morphological reversions were due to inactivation of the v-abl oncogene at multiple steps including transcription, translation or v-abl protein kinase activity itself without any change in structural gene expression of helper virus. These findings suggest the existence of a specific mechanism(s) for elimination of the v-abl oncogene by segregation, mutation, or gene rearrangement in these cells.

Original languageEnglish
Pages (from-to)428-433
Number of pages6
JournalJournal of Cellular Physiology
Volume145
Issue number3
Publication statusPublished - Dec 1990
Externally publishedYes

Fingerprint

Abelson murine leukemia virus
abl Genes
Viruses
Oncogene Proteins v-abl
Helper Viruses
Gene Rearrangement
Transcription
Fibroblasts
Gene expression
Protein Kinases
Rats
Clone Cells
Genes
Cells
Phenotype
Gene Expression
Cell Line
Mutation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Characterization of flat revertants isolated from cells transformed by Abelson murine leukemia virus (Ab-MuLV). / Oka, Takashi; Yutsudo, Masuo; Inoue, Hirokazu; Higuchi, Fujito; Hakura, Akira.

In: Journal of Cellular Physiology, Vol. 145, No. 3, 12.1990, p. 428-433.

Research output: Contribution to journalArticle

Oka, Takashi ; Yutsudo, Masuo ; Inoue, Hirokazu ; Higuchi, Fujito ; Hakura, Akira. / Characterization of flat revertants isolated from cells transformed by Abelson murine leukemia virus (Ab-MuLV). In: Journal of Cellular Physiology. 1990 ; Vol. 145, No. 3. pp. 428-433.
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