TY - JOUR
T1 - Characterization of distinct ovarian isoform of crustacean female sex hormone in the kuruma prawn Marsupenaeus japonicus
AU - Tsutsui, Naoaki
AU - Kotaka, Sayaka
AU - Ohira, Tsuyoshi
AU - Sakamoto, Tatsuya
N1 - Funding Information:
We thank Dr. T. Okumura of the National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency for helpful suggestions in this work. We are also grateful to Mrs. T. Shiokawa of Division of Instrumental Analysis, Okayama University for technical assistance in ESI mass spectrometry. This work was supported in part by Grants-in-Aid for Scientific Research (Nos. 25850143 and 15K07576 ) from the Ministry of Education, Culture, Sport, Science and Technology (MEXT) of Japan and by the SUNBOR GRANT from the Suntory Institute for Bioorganic Research .
Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2018/3
Y1 - 2018/3
N2 - The eyestalk hormone, crustacean female sex hormone (CFSH), regulates the development of female secondary sexual characteristics in the blue crab Callinectes sapidus. After its discovery, several CFSH gene orthologs have been identified in some species of the suborder Pleocyemata as well. Similarly, in species of another suborder (Dendrobranchiata), an ortholog (Maj-CFSH) has been characterized as an eyestalk factor expressed in both females and males of the kuruma prawn, Marsupenaeus japonicus. In this study, another novel CFSH isoform was identified in the same species using cDNA cloning, expression analysis, and recombinant protein production. The isoform has “CFSH-family” structural characteristics but is dominantly expressed in the ovary, and was therefore designated as Maj-CFSH-ov. Its mRNA and protein levels in vitellogenic ovaries are higher than those in non-vitellogenic ovaries. In the vitellogenic ovary, both mRNA and protein expression of Maj-CFSH-ov are localized to oogonia and previtellogenic oocytes that occupy a small portion of vitellogenic ovaries, but not to the major developing oocytes. A vitellogenesis-inhibiting peptide of M. japonicus (Pej-SGP-I) reduced the expression of vitellogenin in incubated ovarian fragments, but not that of Maj-CFSH-ov. These results indicate that M. japonicus possesses two CFSH isoforms that are derived from distinct tissues, the central X-organ/sinus gland complex and peripheral ovaries. The expression profile of Maj-CFSH-ov suggests its involvement in some reproductive process other than vitellogenesis.
AB - The eyestalk hormone, crustacean female sex hormone (CFSH), regulates the development of female secondary sexual characteristics in the blue crab Callinectes sapidus. After its discovery, several CFSH gene orthologs have been identified in some species of the suborder Pleocyemata as well. Similarly, in species of another suborder (Dendrobranchiata), an ortholog (Maj-CFSH) has been characterized as an eyestalk factor expressed in both females and males of the kuruma prawn, Marsupenaeus japonicus. In this study, another novel CFSH isoform was identified in the same species using cDNA cloning, expression analysis, and recombinant protein production. The isoform has “CFSH-family” structural characteristics but is dominantly expressed in the ovary, and was therefore designated as Maj-CFSH-ov. Its mRNA and protein levels in vitellogenic ovaries are higher than those in non-vitellogenic ovaries. In the vitellogenic ovary, both mRNA and protein expression of Maj-CFSH-ov are localized to oogonia and previtellogenic oocytes that occupy a small portion of vitellogenic ovaries, but not to the major developing oocytes. A vitellogenesis-inhibiting peptide of M. japonicus (Pej-SGP-I) reduced the expression of vitellogenin in incubated ovarian fragments, but not that of Maj-CFSH-ov. These results indicate that M. japonicus possesses two CFSH isoforms that are derived from distinct tissues, the central X-organ/sinus gland complex and peripheral ovaries. The expression profile of Maj-CFSH-ov suggests its involvement in some reproductive process other than vitellogenesis.
KW - Crustacean female sex hormone
KW - Marsupenaeus japonicus
KW - Ovary
KW - Reproduction
KW - Vitellogenesis
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U2 - 10.1016/j.cbpa.2017.12.009
DO - 10.1016/j.cbpa.2017.12.009
M3 - Article
C2 - 29277431
AN - SCOPUS:85038843870
VL - 217
SP - 7
EP - 16
JO - Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
JF - Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
SN - 1095-6433
ER -