Characterization of disease resistance gene-like sequences in near-isogenic lines of tomato

T. Ohmori, M. Murata, F. Motoyoshi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We examined near-isogenic lines (NILs) carrying either of the tomato mosaic virus (ToMV) resistance genes Tm-1 and Tm-2 for sequences homologous to the isolated disease-resistance genes. DNA fragments were amplified from the genomic DNA of the NILs by the polymerase chain reaction (PCR) using primers designed on the basis of sequences of certain domains conserved among some disease-resistance genes. Of ten PCR products cloned, five were identified as having homology to either of the two classes of disease-resistance genes. The first class encoded proteins containing leucine-rich repeats (LRRs) and a nucleotide-binding site (NBS), such as the RPS2 gene in Arabidopsis and the N gene in tobacco. The second class encoded proteins containing a C-terminal membrane anchor but no NBS, such as the Cf2 and Cf9 genes in tomato. In Southern hybridization of the genomic DNAs of the NILs carrying either Tm-1 or Tm-2 and their parental NIL carrying neither of these resistance genes, multiple bands could be detected with most of the clones used as probes. This suggests that the genomes of the NILs contain multiple copies of sequences homologous to some of the known disease-resistance genes. No evidence was obtained to show that the Tm-1 and/or Tm-2 loci encode either class of protein, since no polymorphic band patterns between the NILs were detected by Southern hybridization.

Original languageEnglish
Pages (from-to)331-338
Number of pages8
JournalTheoretical And Applied Genetics
Volume96
Issue number3-4
DOIs
Publication statusPublished - Mar 1998

Fingerprint

Disease Resistance
isogenic lines
Lycopersicon esculentum
disease resistance
tomatoes
Genes
genes
Sequence Homology
sequence homology
Southern blotting
binding sites
DNA
Nucleotides
polymerase chain reaction
nucleotides
Binding Sites
Tomato mosaic virus
Nucleic Acid Hybridization
genomics
Mosaic Viruses

Keywords

  • Disease-resistance gene
  • Lycopersicon esculentum
  • Near-isogenic line (NIL)
  • Polymorphism
  • Tomato mosaic virus (ToMV)

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Genetics
  • Genetics(clinical)

Cite this

Characterization of disease resistance gene-like sequences in near-isogenic lines of tomato. / Ohmori, T.; Murata, M.; Motoyoshi, F.

In: Theoretical And Applied Genetics, Vol. 96, No. 3-4, 03.1998, p. 331-338.

Research output: Contribution to journalArticle

Ohmori, T. ; Murata, M. ; Motoyoshi, F. / Characterization of disease resistance gene-like sequences in near-isogenic lines of tomato. In: Theoretical And Applied Genetics. 1998 ; Vol. 96, No. 3-4. pp. 331-338.
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