Characterization of dermacan, a novel zebrafish lectican gene, expressed in dermal bones

Jeong Suk Kang, Toshitaka Oohashi, Yasuhiko Kawakami, Yoko Bekku, Juan Carlos Izpisúa Belmonte, Yoshifumi Ninomiya

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We report here the isolation and characterization of a cDNA encoding zebrafish dermacan, a novel member of hyaluronan (HA)-binding proteoglycans, which was termed after its characteristic expression in the zebrafish dermal bones. The deduced protein sequence shares the typical modular elements of lecticans. Sequence comparison covering the C-terminal globular domain demonstrated that dermacan shows high homology with zebrafish versican but is distinct from any other identified lecticans. Genomic DNA analysis demonstrated that dermacan and versican were encoded by distinct genes in the zebrafish genome. The expression of dermacan is initiated in the sclerotome and cephalic paraxial mesoderm at 16 h postfertilization. During the pharyngular period, dermacan transcripts were detected in the sclerotome, tail fin bud, pharyngular arch primordial region, and otic vesicle. In the development of craniofacial bones, dermacan expression was detected typically in the opercle and dentary. These regions belong to the craniofacial dermal bones. aggrecan expression, in contrast, was observed in the elements of craniofacial cartilage bones. In the dermacan-morpholino-injected embryos, dermal bones, e.g. opercle, dentary, and branchiostegal rays, as well as axial skeleton in the trunk, showed decreased ossification. We conclude that dermacan is a novel lectican gene, and that zebrafish lectican genes have genetically diverged. In addition, our data suggest the involvement of dermacan in zebrafish dermal bone development.

Original languageEnglish
Pages (from-to)301-312
Number of pages12
JournalMechanisms of Development
Volume121
Issue number3
DOIs
Publication statusPublished - Mar 2004

Fingerprint

Zebrafish
Versicans
Bone and Bones
Skin
Bone Development
Genes
Aggrecans
Morpholinos
Mesoderm
Proteoglycans
Hyaluronic Acid
Osteogenesis
Skeleton
Cartilage
Ear
Tail
Embryonic Structures
Complementary DNA
Head
Genome

Keywords

  • Aggrecan
  • Branchiostegal ray
  • Craniofacial development
  • Dermacan
  • Dermal bone
  • Lectican
  • Morpholino
  • Opercle
  • Versican
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Characterization of dermacan, a novel zebrafish lectican gene, expressed in dermal bones. / Kang, Jeong Suk; Oohashi, Toshitaka; Kawakami, Yasuhiko; Bekku, Yoko; Izpisúa Belmonte, Juan Carlos; Ninomiya, Yoshifumi.

In: Mechanisms of Development, Vol. 121, No. 3, 03.2004, p. 301-312.

Research output: Contribution to journalArticle

Kang, JS, Oohashi, T, Kawakami, Y, Bekku, Y, Izpisúa Belmonte, JC & Ninomiya, Y 2004, 'Characterization of dermacan, a novel zebrafish lectican gene, expressed in dermal bones', Mechanisms of Development, vol. 121, no. 3, pp. 301-312. https://doi.org/10.1016/j.mod.2004.01.007
Kang, Jeong Suk ; Oohashi, Toshitaka ; Kawakami, Yasuhiko ; Bekku, Yoko ; Izpisúa Belmonte, Juan Carlos ; Ninomiya, Yoshifumi. / Characterization of dermacan, a novel zebrafish lectican gene, expressed in dermal bones. In: Mechanisms of Development. 2004 ; Vol. 121, No. 3. pp. 301-312.
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