Characterization of CENH3 and centromere-associated DNA sequences in sugarcane

Kiyotaka Nagaki, Minoru Murata

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    66 Citations (Scopus)

    Abstract

    Centromere-specific histone H3 (CENH3) has been used to detect active centromeres, and to analyse the DNA sequences closely associated with the centromere, because they localize only in active centromeres and bind directly to the DNA. In maize and rice, the centromeric retrotransposons (CR) are shown to be closely associated with their own CENH3 whereas no such association was found in Arabidopsis thaliana. In this study, this sort of association was investigated in sugarcane. Two expressed sequence tag groups encoding putative sugarcane CENH3 (SoCENH3) were found in a sugarcane-expressed sequence tag database. Their deduced amino acid sequences were similar to these of the CENH3s in rice and maize. An antibody against rice CENH3 seemed to crossreact with the SoCENH3s, and stained sugarcane centromeres. A set of immunoprecipitation tests was conducted with the antibody and chromatin from the sugarcane genome to reveal CENH3-associated DNA sequences in sugarcane. Centromeric tandem repeats (SCEN) and centromeric retrotransposons of sugarcane (CRS) were significantly precipitated with the antibody, meaning these repeats are directly interacting with CENH3 in sugarcane centromeres.

    Original languageEnglish
    Pages (from-to)195-203
    Number of pages9
    JournalChromosome Research
    Volume13
    Issue number2
    DOIs
    Publication statusPublished - Jan 2005

    Keywords

    • CENH3
    • CR
    • Centromere
    • ChIP

    ASJC Scopus subject areas

    • Genetics

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