Characterization of CENH3 and centromere-associated DNA sequences in sugarcane

Kiyotaka Nagaki, Minoru Murata

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Centromere-specific histone H3 (CENH3) has been used to detect active centromeres, and to analyse the DNA sequences closely associated with the centromere, because they localize only in active centromeres and bind directly to the DNA. In maize and rice, the centromeric retrotransposons (CR) are shown to be closely associated with their own CENH3 whereas no such association was found in Arabidopsis thaliana. In this study, this sort of association was investigated in sugarcane. Two expressed sequence tag groups encoding putative sugarcane CENH3 (SoCENH3) were found in a sugarcane-expressed sequence tag database. Their deduced amino acid sequences were similar to these of the CENH3s in rice and maize. An antibody against rice CENH3 seemed to crossreact with the SoCENH3s, and stained sugarcane centromeres. A set of immunoprecipitation tests was conducted with the antibody and chromatin from the sugarcane genome to reveal CENH3-associated DNA sequences in sugarcane. Centromeric tandem repeats (SCEN) and centromeric retrotransposons of sugarcane (CRS) were significantly precipitated with the antibody, meaning these repeats are directly interacting with CENH3 in sugarcane centromeres.

Original languageEnglish
Pages (from-to)195-203
Number of pages9
JournalChromosome Research
Volume13
Issue number2
DOIs
Publication statusPublished - Jan 2005

Fingerprint

Saccharum
Centromere
Histones
Retroelements
Expressed Sequence Tags
Zea mays
Antibodies
Tandem Repeat Sequences
DNA Sequence Analysis
Immunoprecipitation
Arabidopsis
Chromatin
Amino Acid Sequence
Genome
Databases

Keywords

  • CENH3
  • Centromere
  • ChIP
  • CR

ASJC Scopus subject areas

  • Genetics

Cite this

Characterization of CENH3 and centromere-associated DNA sequences in sugarcane. / Nagaki, Kiyotaka; Murata, Minoru.

In: Chromosome Research, Vol. 13, No. 2, 01.2005, p. 195-203.

Research output: Contribution to journalArticle

@article{c77582e4ef514bdcae8f7bab7360aa2a,
title = "Characterization of CENH3 and centromere-associated DNA sequences in sugarcane",
abstract = "Centromere-specific histone H3 (CENH3) has been used to detect active centromeres, and to analyse the DNA sequences closely associated with the centromere, because they localize only in active centromeres and bind directly to the DNA. In maize and rice, the centromeric retrotransposons (CR) are shown to be closely associated with their own CENH3 whereas no such association was found in Arabidopsis thaliana. In this study, this sort of association was investigated in sugarcane. Two expressed sequence tag groups encoding putative sugarcane CENH3 (SoCENH3) were found in a sugarcane-expressed sequence tag database. Their deduced amino acid sequences were similar to these of the CENH3s in rice and maize. An antibody against rice CENH3 seemed to crossreact with the SoCENH3s, and stained sugarcane centromeres. A set of immunoprecipitation tests was conducted with the antibody and chromatin from the sugarcane genome to reveal CENH3-associated DNA sequences in sugarcane. Centromeric tandem repeats (SCEN) and centromeric retrotransposons of sugarcane (CRS) were significantly precipitated with the antibody, meaning these repeats are directly interacting with CENH3 in sugarcane centromeres.",
keywords = "CENH3, Centromere, ChIP, CR",
author = "Kiyotaka Nagaki and Minoru Murata",
year = "2005",
month = "1",
doi = "10.1007/s10577-005-0847-2",
language = "English",
volume = "13",
pages = "195--203",
journal = "Chromosome Research",
issn = "0967-3849",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Characterization of CENH3 and centromere-associated DNA sequences in sugarcane

AU - Nagaki, Kiyotaka

AU - Murata, Minoru

PY - 2005/1

Y1 - 2005/1

N2 - Centromere-specific histone H3 (CENH3) has been used to detect active centromeres, and to analyse the DNA sequences closely associated with the centromere, because they localize only in active centromeres and bind directly to the DNA. In maize and rice, the centromeric retrotransposons (CR) are shown to be closely associated with their own CENH3 whereas no such association was found in Arabidopsis thaliana. In this study, this sort of association was investigated in sugarcane. Two expressed sequence tag groups encoding putative sugarcane CENH3 (SoCENH3) were found in a sugarcane-expressed sequence tag database. Their deduced amino acid sequences were similar to these of the CENH3s in rice and maize. An antibody against rice CENH3 seemed to crossreact with the SoCENH3s, and stained sugarcane centromeres. A set of immunoprecipitation tests was conducted with the antibody and chromatin from the sugarcane genome to reveal CENH3-associated DNA sequences in sugarcane. Centromeric tandem repeats (SCEN) and centromeric retrotransposons of sugarcane (CRS) were significantly precipitated with the antibody, meaning these repeats are directly interacting with CENH3 in sugarcane centromeres.

AB - Centromere-specific histone H3 (CENH3) has been used to detect active centromeres, and to analyse the DNA sequences closely associated with the centromere, because they localize only in active centromeres and bind directly to the DNA. In maize and rice, the centromeric retrotransposons (CR) are shown to be closely associated with their own CENH3 whereas no such association was found in Arabidopsis thaliana. In this study, this sort of association was investigated in sugarcane. Two expressed sequence tag groups encoding putative sugarcane CENH3 (SoCENH3) were found in a sugarcane-expressed sequence tag database. Their deduced amino acid sequences were similar to these of the CENH3s in rice and maize. An antibody against rice CENH3 seemed to crossreact with the SoCENH3s, and stained sugarcane centromeres. A set of immunoprecipitation tests was conducted with the antibody and chromatin from the sugarcane genome to reveal CENH3-associated DNA sequences in sugarcane. Centromeric tandem repeats (SCEN) and centromeric retrotransposons of sugarcane (CRS) were significantly precipitated with the antibody, meaning these repeats are directly interacting with CENH3 in sugarcane centromeres.

KW - CENH3

KW - Centromere

KW - ChIP

KW - CR

UR - http://www.scopus.com/inward/record.url?scp=18744386414&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18744386414&partnerID=8YFLogxK

U2 - 10.1007/s10577-005-0847-2

DO - 10.1007/s10577-005-0847-2

M3 - Article

C2 - 15861308

AN - SCOPUS:18744386414

VL - 13

SP - 195

EP - 203

JO - Chromosome Research

JF - Chromosome Research

SN - 0967-3849

IS - 2

ER -