Characterization of bone marrow derived mesenchymal stem cells in suspension

Kentaro Akiyama, Yong Ouk You, Takayoshi Yamaza, Chider Chen, Liang Tang, Yan Jin, Xiao Dong Chen, Stan Gronthos, Songtao Shi

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Introduction. Bone marrow mesenchymal stem cells (BMMSCs) are a heterogeneous population of postnatal precursor cells with the capacity of adhering to culture dishes generating colony-forming unit-fibroblasts (CFU-F). Here we identify a new subset of BMMSCs that fail to adhere to plastic culture dishes and remain in culture suspension (S-BMMSCs). Methods. To catch S-BMMSCs, we used BMMSCs-produced extracellular cell matrix (ECM)-coated dishes. Isolated S-BMMSCs were analyzed by in vitro stem cell analysis approaches, including flow cytometry, inductive multiple differentiation, western blot and in vivo implantation to assess the bone regeneration ability of S-BMMSCs. Furthermore, we performed systemic S-BMMSCs transplantation to treat systemic lupus erythematosus (SLE)-like MRL/lpr mice. Results: S-BMMSCs are capable of adhering to ECM-coated dishes and showing mesenchymal stem cell characteristics with distinction from hematopoietic cells as evidenced by co-expression of CD73 or Oct-4 with CD34, forming a single colony cluster on ECM, and failure to differentiate into hematopoietic cell lineage. Moreover, we found that culture-expanded S-BMMSCs exhibited significantly increased immunomodulatory capacities in vitro and an efficacious treatment for SLE-like MRL/lpr mice by rebalancing regulatory T cells (Tregs) and T helper 17 cells (Th17) through high NO production. Conclusions: These data suggest that it is feasible to improve immunotherapy by identifying a new subset BMMSCs.

Original languageEnglish
Article number40
JournalStem Cell Research and Therapy
Volume3
Issue number5
DOIs
Publication statusPublished - 2012

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Stem cells
Mesenchymal Stromal Cells
Suspensions
Bone
Bone Marrow
Cell culture
Inbred MRL lpr Mouse
Extracellular Matrix
Systemic Lupus Erythematosus
Stem Cells
Mesenchymal Stem Cell Transplantation
Th17 Cells
Bone Regeneration
Cell Lineage
Regulatory T-Lymphocytes
T-cells
Flow cytometry
Immunotherapy
Plastics
Fibroblasts

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Molecular Medicine
  • Cell Biology
  • Medicine (miscellaneous)
  • Medicine(all)

Cite this

Characterization of bone marrow derived mesenchymal stem cells in suspension. / Akiyama, Kentaro; You, Yong Ouk; Yamaza, Takayoshi; Chen, Chider; Tang, Liang; Jin, Yan; Chen, Xiao Dong; Gronthos, Stan; Shi, Songtao.

In: Stem Cell Research and Therapy, Vol. 3, No. 5, 40, 2012.

Research output: Contribution to journalArticle

Akiyama, K, You, YO, Yamaza, T, Chen, C, Tang, L, Jin, Y, Chen, XD, Gronthos, S & Shi, S 2012, 'Characterization of bone marrow derived mesenchymal stem cells in suspension', Stem Cell Research and Therapy, vol. 3, no. 5, 40. https://doi.org/10.1186/scrt131
Akiyama, Kentaro ; You, Yong Ouk ; Yamaza, Takayoshi ; Chen, Chider ; Tang, Liang ; Jin, Yan ; Chen, Xiao Dong ; Gronthos, Stan ; Shi, Songtao. / Characterization of bone marrow derived mesenchymal stem cells in suspension. In: Stem Cell Research and Therapy. 2012 ; Vol. 3, No. 5.
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