Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus: A Dethiobiotin producing Bacterium

Yoshikazu Izumi, Yoshiaki Kano, Kenji Inagaki, Naoki Kawase, Yoshiki Tani, Hideaki Yamada

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A variety of bacteria and yeasts were examined for activities of biotin biosynthetic enzymes, including pimelyl-CoA synthetase, 7-keto-8-aminopelargonic acid (KAPA) synthetase, 7,8-diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin (DTB) synthetase. Among the strains tested, only Bacillus sphaericus, a DTB producer, showed significant activities for all four enzymes. The bacterium also exhibited high activity of biotin synthesis from DTB in an intact cell system. Using cell-free extract and intact cells, some properties of DAPA aminotransferase, DTB synthetase and biotin synthesizing reaction were examined. Based on these results of enzyme activities DTB productivity of B. sphaericus was discussed.

Original languageEnglish
Pages (from-to)1983-1989
Number of pages7
JournalAgricultural and Biological Chemistry
Issue number9
Publication statusPublished - 1981
Externally publishedYes


ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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