Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus: A Dethiobiotin producing Bacterium

Yoshikazu Izumi, Yoshiaki Kano, Kenji Inagaki, Naoki Kawase, Yoshiki Tani, Hideaki Yamada

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A variety of bacteria and yeasts were examined for activities of biotin biosynthetic enzymes, including pimelyl-CoA synthetase, 7-keto-8-aminopelargonic acid (KAPA) synthetase, 7,8-diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin (DTB) synthetase. Among the strains tested, only Bacillus sphaericus, a DTB producer, showed significant activities for all four enzymes. The bacterium also exhibited high activity of biotin synthesis from DTB in an intact cell system. Using cell-free extract and intact cells, some properties of DAPA aminotransferase, DTB synthetase and biotin synthesizing reaction were examined. Based on these results of enzyme activities DTB productivity of B. sphaericus was discussed.

Original languageEnglish
Pages (from-to)1983-1989
Number of pages7
JournalAgricultural and Biological Chemistry
Volume45
Issue number9
DOIs
Publication statusPublished - 1981
Externally publishedYes

Fingerprint

dethiobiotin synthetase
Bacillus sphaericus
Bacilli
biotin
Biotin
ligases
Bacillus
Bacteria
8-amino-7-oxononanoate synthase
bacteria
transaminases
Enzymes
enzymes
acids
Enzyme activity
Cell Extracts
Yeast
cells
Yeasts
Productivity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus : A Dethiobiotin producing Bacterium. / Izumi, Yoshikazu; Kano, Yoshiaki; Inagaki, Kenji; Kawase, Naoki; Tani, Yoshiki; Yamada, Hideaki.

In: Agricultural and Biological Chemistry, Vol. 45, No. 9, 1981, p. 1983-1989.

Research output: Contribution to journalArticle

Izumi, Yoshikazu ; Kano, Yoshiaki ; Inagaki, Kenji ; Kawase, Naoki ; Tani, Yoshiki ; Yamada, Hideaki. / Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus : A Dethiobiotin producing Bacterium. In: Agricultural and Biological Chemistry. 1981 ; Vol. 45, No. 9. pp. 1983-1989.
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