Characterization of biotin biosynthetic enzymes of bacillus sphaericus: A dethiobiotin producing bacterium

Yoshikazu Izumi; Yoshiaki Kano; Kenji Inagaki; Naoki Kawase; Yoshiki Tani; Hideaki Yamada

doi:10.1080/00021369.1981.10864833

Characterization of biotin biosynthetic enzymes of bacillus sphaericus: A dethiobiotin producing bacterium

Yoshikazu Izumi, Yoshiaki Kano, Kenji Inagaki, Naoki Kawase, Yoshiki Tani, Hideaki Yamada

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

A variety of bacteria and yeasts were examined for activities of biotin biosynthetic enzymes, including pimelyl-CoA synthetase, 7-keto-8-aminopelargonic acid (KAPA) synthetase, 7, 8- diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin (DTB) synthetase. Among the strains tested, only Bacillus sphaericus, a DTB producer, showed significant activities for all four enzymes. The bacterium also exhibited high activity of biotin synthesis from DTB in an intact cell system. Using cell-free extract and intact cells, some properties of DAP A aminotransferase, DTB synthetase and biotin synthesizing reaction were examined.Based on these results of enzyme activities DTB productivity of B. sphaericus was discussed.

Original language	English
Pages (from-to)	1983-1989
Number of pages	7
Journal	Agricultural and Biological Chemistry
Volume	45
Issue number	9
DOIs	https://doi.org/10.1080/00021369.1981.10864833
Publication status	Published - Sept 1981
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

Access to Document

10.1080/00021369.1981.10864833

Cite this

@article{740900f66e73466893c0dafa701d88d0,

title = "Characterization of biotin biosynthetic enzymes of bacillus sphaericus: A dethiobiotin producing bacterium",

abstract = "A variety of bacteria and yeasts were examined for activities of biotin biosynthetic enzymes, including pimelyl-CoA synthetase, 7-keto-8-aminopelargonic acid (KAPA) synthetase, 7, 8- diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin (DTB) synthetase. Among the strains tested, only Bacillus sphaericus, a DTB producer, showed significant activities for all four enzymes. The bacterium also exhibited high activity of biotin synthesis from DTB in an intact cell system. Using cell-free extract and intact cells, some properties of DAP A aminotransferase, DTB synthetase and biotin synthesizing reaction were examined.Based on these results of enzyme activities DTB productivity of B. sphaericus was discussed.",

author = "Yoshikazu Izumi and Yoshiaki Kano and Kenji Inagaki and Naoki Kawase and Yoshiki Tani and Hideaki Yamada",

year = "1981",

month = sep,

doi = "10.1080/00021369.1981.10864833",

language = "English",

volume = "45",

pages = "1983--1989",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "9",

}

TY - JOUR

T1 - Characterization of biotin biosynthetic enzymes of bacillus sphaericus

T2 - A dethiobiotin producing bacterium

AU - Izumi, Yoshikazu

AU - Kano, Yoshiaki

AU - Inagaki, Kenji

AU - Kawase, Naoki

AU - Tani, Yoshiki

AU - Yamada, Hideaki

PY - 1981/9

Y1 - 1981/9

N2 - A variety of bacteria and yeasts were examined for activities of biotin biosynthetic enzymes, including pimelyl-CoA synthetase, 7-keto-8-aminopelargonic acid (KAPA) synthetase, 7, 8- diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin (DTB) synthetase. Among the strains tested, only Bacillus sphaericus, a DTB producer, showed significant activities for all four enzymes. The bacterium also exhibited high activity of biotin synthesis from DTB in an intact cell system. Using cell-free extract and intact cells, some properties of DAP A aminotransferase, DTB synthetase and biotin synthesizing reaction were examined.Based on these results of enzyme activities DTB productivity of B. sphaericus was discussed.

AB - A variety of bacteria and yeasts were examined for activities of biotin biosynthetic enzymes, including pimelyl-CoA synthetase, 7-keto-8-aminopelargonic acid (KAPA) synthetase, 7, 8- diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin (DTB) synthetase. Among the strains tested, only Bacillus sphaericus, a DTB producer, showed significant activities for all four enzymes. The bacterium also exhibited high activity of biotin synthesis from DTB in an intact cell system. Using cell-free extract and intact cells, some properties of DAP A aminotransferase, DTB synthetase and biotin synthesizing reaction were examined.Based on these results of enzyme activities DTB productivity of B. sphaericus was discussed.

UR - http://www.scopus.com/inward/record.url?scp=0000768094&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000768094&partnerID=8YFLogxK

U2 - 10.1080/00021369.1981.10864833

DO - 10.1080/00021369.1981.10864833

M3 - Article

AN - SCOPUS:0000768094

SN - 0916-8451

VL - 45

SP - 1983

EP - 1989

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 9

ER -

Characterization of biotin biosynthetic enzymes of bacillus sphaericus: A dethiobiotin producing bacterium

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this