Characterization of biotin biosynthetic enzymes of bacillus sphaericus: A dethiobiotin producing bacterium

Yoshikazu Izumi, Yoshiaki Kano, Kenji Inagaki, Naoki Kawase, Yoshiki Tani, Hideaki Yamada

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

A variety of bacteria and yeasts were examined for activities of biotin biosynthetic enzymes, including pimelyl-CoA synthetase, 7-keto-8-aminopelargonic acid (KAPA) synthetase, 7, 8- diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin (DTB) synthetase. Among the strains tested, only Bacillus sphaericus, a DTB producer, showed significant activities for all four enzymes. The bacterium also exhibited high activity of biotin synthesis from DTB in an intact cell system. Using cell-free extract and intact cells, some properties of DAP A aminotransferase, DTB synthetase and biotin synthesizing reaction were examined.Based on these results of enzyme activities DTB productivity of B. sphaericus was discussed.

Original languageEnglish
Pages (from-to)1983-1989
Number of pages7
JournalAgricultural and Biological Chemistry
Volume45
Issue number9
DOIs
Publication statusPublished - Sep 1981
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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