Characterization of an avian histidine decarboxylase and localization of histaminergic neurons in the chicken brain

Yuki Bessho, Eiko Iwakoshi-Ukena, Tetsuya Tachibana, Sho Maejima, Shusuke Taniuchi, Keiko Masuda, Kenshiro Shikano, Kunihiro Kondo, Megumi Furumitsu, Kazuyoshi Ukena

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In mammals, it is established that histamine is a neurotransmitter and/or neuromodulator in the central nervous system. It is produced by the enzyme histidine decarboxylase (HDC) in the tuberomammillary nucleus of the posterior hypothalamus. However, HDC as well as histaminergic neurons have not yet been characterized in the avian brain. We have cloned the cDNA for HDC from the chicken hypothalamus and demonstrated that the chicken HDC sequence is highly homologous to the mammalian counterpart, and that the expressed protein shows high enzymatic activity. The expression of HDC mRNA at various sites in the brain was investigated using quantitative RT-PCR. The results showed that the HDC mRNA was highly expressed in the hypothalamic infundibulum. In situ hybridization analyses revealed that the cells containing HDC mRNA were localized in the medial mammillary nucleus of the hypothalamic infundibulum. Intracerebroventricular injection of histamine in chicks resulted in inhibition of feeding behavior. This is the first report of the characterization of histaminergic neurons in the avian brain, and our findings indicate that neuronal histamine exerts anorexigenic effects in chicks.

Original languageEnglish
Pages (from-to)106-110
Number of pages5
JournalNeuroscience Letters
Volume578
DOIs
Publication statusPublished - Aug 22 2014
Externally publishedYes

Fingerprint

Histidine Decarboxylase
Chickens
Neurons
Brain
Histamine
Pituitary Gland
Messenger RNA
Neurotransmitter Agents
Lateral Hypothalamic Area
Posterior Hypothalamus
Feeding Behavior
Hypothalamus
In Situ Hybridization
Mammals
Central Nervous System
Complementary DNA
Polymerase Chain Reaction
Injections
Enzymes

Keywords

  • Brain
  • Chicken
  • Food intake
  • Histamine
  • Histidine decarboxylase
  • Hypothalamus

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Characterization of an avian histidine decarboxylase and localization of histaminergic neurons in the chicken brain. / Bessho, Yuki; Iwakoshi-Ukena, Eiko; Tachibana, Tetsuya; Maejima, Sho; Taniuchi, Shusuke; Masuda, Keiko; Shikano, Kenshiro; Kondo, Kunihiro; Furumitsu, Megumi; Ukena, Kazuyoshi.

In: Neuroscience Letters, Vol. 578, 22.08.2014, p. 106-110.

Research output: Contribution to journalArticle

Bessho, Y, Iwakoshi-Ukena, E, Tachibana, T, Maejima, S, Taniuchi, S, Masuda, K, Shikano, K, Kondo, K, Furumitsu, M & Ukena, K 2014, 'Characterization of an avian histidine decarboxylase and localization of histaminergic neurons in the chicken brain', Neuroscience Letters, vol. 578, pp. 106-110. https://doi.org/10.1016/j.neulet.2014.06.048
Bessho, Yuki ; Iwakoshi-Ukena, Eiko ; Tachibana, Tetsuya ; Maejima, Sho ; Taniuchi, Shusuke ; Masuda, Keiko ; Shikano, Kenshiro ; Kondo, Kunihiro ; Furumitsu, Megumi ; Ukena, Kazuyoshi. / Characterization of an avian histidine decarboxylase and localization of histaminergic neurons in the chicken brain. In: Neuroscience Letters. 2014 ; Vol. 578. pp. 106-110.
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