Characterization of an anion transporter in the plasma membrane of barley roots

Kousei Yamashita, Yoko Yamamoto, Hideaki Matsumoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To examine the relationship between H+-ATPase and the transport of anions, we investigated the effects of various inhibitors on the activity of the H+-ATPase, the transport of protons, and the transport of Cl- ions using plasma membrane vesicles prepared from barley roots. Some inhibitors, namely, 4,4-diisothiocyano-2,2-stilbene disulfonate (DIDS) and Zn2+ ions markedly inhibited H+-ATPase activity. Other compounds, such as phenylglyoxal (PGO) and niflumic acid (NIF), inhibited H+-ATPase activity by 20-30%, while anthracene-9-carboxylate (A-9-C) and tetraethylammonium chloride (TEA-Cl) had little effect on this activity. The ATP-dependent acidification of the interior of vesicles was strongly dependent on the presence of permeant anions, such as chloride (Cl-) and nitrate (NO3/-), and it was completely inhibited by 0.2 mM NIF. Other compounds, namely, A-9- C of 0.1 mM and TEA-Cl of 10 mM, did not affect H+-transport activity. The inhibition of H+-transport activity by NIF was observed even when the activity was assayed in the presence of KCl, KNO3, or bis-tris-propane (BTP)-Cl. Using 36Cl-, we quantified Cl--transport activity by measuring the uptake of Cl- ions into the plasma membrane vesicles. The uptake depended on the potential difference across the membrane that was generated by H+-ATPase; it was enhanced by an inside-positive potential gradient. At 0.1 mM, NIF completely blocked the voltage-dependent Cl--transport activity. From these properties of the Cl- transporter and the inhibition of H+transport activity by NIF, we suggest that H+-transport activity across the plasma membrane might be modulated by the transport of anions via a NIF- sensitive anion-permeable transporter that acts to collapse the inside- positive potential generated by H+-ATPase.

Original languageEnglish
Pages (from-to)949-956
Number of pages8
JournalPlant and Cell Physiology
Volume37
Issue number7
Publication statusPublished - Oct 1996

Fingerprint

Niflumic Acid
Proton-Translocating ATPases
Hordeum
anions
H-transporting ATP synthase
Anions
transporters
Chlorides
chlorides
plasma membrane
barley
Cell Membrane
acids
Tetraethylammonium
ions
Phenylglyoxal
Ions
Stilbenes
Ion Transport
Nitrates

Keywords

  • Cl uptake
  • H-transport activity
  • Hordeum vulgare L.
  • Niflumic acid
  • Plasma membrane

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Physiology

Cite this

Yamashita, K., Yamamoto, Y., & Matsumoto, H. (1996). Characterization of an anion transporter in the plasma membrane of barley roots. Plant and Cell Physiology, 37(7), 949-956.

Characterization of an anion transporter in the plasma membrane of barley roots. / Yamashita, Kousei; Yamamoto, Yoko; Matsumoto, Hideaki.

In: Plant and Cell Physiology, Vol. 37, No. 7, 10.1996, p. 949-956.

Research output: Contribution to journalArticle

Yamashita, K, Yamamoto, Y & Matsumoto, H 1996, 'Characterization of an anion transporter in the plasma membrane of barley roots', Plant and Cell Physiology, vol. 37, no. 7, pp. 949-956.
Yamashita, Kousei ; Yamamoto, Yoko ; Matsumoto, Hideaki. / Characterization of an anion transporter in the plasma membrane of barley roots. In: Plant and Cell Physiology. 1996 ; Vol. 37, No. 7. pp. 949-956.
@article{977fa0f14b0b49c98c01c018b117d5e1,
title = "Characterization of an anion transporter in the plasma membrane of barley roots",
abstract = "To examine the relationship between H+-ATPase and the transport of anions, we investigated the effects of various inhibitors on the activity of the H+-ATPase, the transport of protons, and the transport of Cl- ions using plasma membrane vesicles prepared from barley roots. Some inhibitors, namely, 4,4-diisothiocyano-2,2-stilbene disulfonate (DIDS) and Zn2+ ions markedly inhibited H+-ATPase activity. Other compounds, such as phenylglyoxal (PGO) and niflumic acid (NIF), inhibited H+-ATPase activity by 20-30{\%}, while anthracene-9-carboxylate (A-9-C) and tetraethylammonium chloride (TEA-Cl) had little effect on this activity. The ATP-dependent acidification of the interior of vesicles was strongly dependent on the presence of permeant anions, such as chloride (Cl-) and nitrate (NO3/-), and it was completely inhibited by 0.2 mM NIF. Other compounds, namely, A-9- C of 0.1 mM and TEA-Cl of 10 mM, did not affect H+-transport activity. The inhibition of H+-transport activity by NIF was observed even when the activity was assayed in the presence of KCl, KNO3, or bis-tris-propane (BTP)-Cl. Using 36Cl-, we quantified Cl--transport activity by measuring the uptake of Cl- ions into the plasma membrane vesicles. The uptake depended on the potential difference across the membrane that was generated by H+-ATPase; it was enhanced by an inside-positive potential gradient. At 0.1 mM, NIF completely blocked the voltage-dependent Cl--transport activity. From these properties of the Cl- transporter and the inhibition of H+transport activity by NIF, we suggest that H+-transport activity across the plasma membrane might be modulated by the transport of anions via a NIF- sensitive anion-permeable transporter that acts to collapse the inside- positive potential generated by H+-ATPase.",
keywords = "Cl uptake, H-transport activity, Hordeum vulgare L., Niflumic acid, Plasma membrane",
author = "Kousei Yamashita and Yoko Yamamoto and Hideaki Matsumoto",
year = "1996",
month = "10",
language = "English",
volume = "37",
pages = "949--956",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "7",

}

TY - JOUR

T1 - Characterization of an anion transporter in the plasma membrane of barley roots

AU - Yamashita, Kousei

AU - Yamamoto, Yoko

AU - Matsumoto, Hideaki

PY - 1996/10

Y1 - 1996/10

N2 - To examine the relationship between H+-ATPase and the transport of anions, we investigated the effects of various inhibitors on the activity of the H+-ATPase, the transport of protons, and the transport of Cl- ions using plasma membrane vesicles prepared from barley roots. Some inhibitors, namely, 4,4-diisothiocyano-2,2-stilbene disulfonate (DIDS) and Zn2+ ions markedly inhibited H+-ATPase activity. Other compounds, such as phenylglyoxal (PGO) and niflumic acid (NIF), inhibited H+-ATPase activity by 20-30%, while anthracene-9-carboxylate (A-9-C) and tetraethylammonium chloride (TEA-Cl) had little effect on this activity. The ATP-dependent acidification of the interior of vesicles was strongly dependent on the presence of permeant anions, such as chloride (Cl-) and nitrate (NO3/-), and it was completely inhibited by 0.2 mM NIF. Other compounds, namely, A-9- C of 0.1 mM and TEA-Cl of 10 mM, did not affect H+-transport activity. The inhibition of H+-transport activity by NIF was observed even when the activity was assayed in the presence of KCl, KNO3, or bis-tris-propane (BTP)-Cl. Using 36Cl-, we quantified Cl--transport activity by measuring the uptake of Cl- ions into the plasma membrane vesicles. The uptake depended on the potential difference across the membrane that was generated by H+-ATPase; it was enhanced by an inside-positive potential gradient. At 0.1 mM, NIF completely blocked the voltage-dependent Cl--transport activity. From these properties of the Cl- transporter and the inhibition of H+transport activity by NIF, we suggest that H+-transport activity across the plasma membrane might be modulated by the transport of anions via a NIF- sensitive anion-permeable transporter that acts to collapse the inside- positive potential generated by H+-ATPase.

AB - To examine the relationship between H+-ATPase and the transport of anions, we investigated the effects of various inhibitors on the activity of the H+-ATPase, the transport of protons, and the transport of Cl- ions using plasma membrane vesicles prepared from barley roots. Some inhibitors, namely, 4,4-diisothiocyano-2,2-stilbene disulfonate (DIDS) and Zn2+ ions markedly inhibited H+-ATPase activity. Other compounds, such as phenylglyoxal (PGO) and niflumic acid (NIF), inhibited H+-ATPase activity by 20-30%, while anthracene-9-carboxylate (A-9-C) and tetraethylammonium chloride (TEA-Cl) had little effect on this activity. The ATP-dependent acidification of the interior of vesicles was strongly dependent on the presence of permeant anions, such as chloride (Cl-) and nitrate (NO3/-), and it was completely inhibited by 0.2 mM NIF. Other compounds, namely, A-9- C of 0.1 mM and TEA-Cl of 10 mM, did not affect H+-transport activity. The inhibition of H+-transport activity by NIF was observed even when the activity was assayed in the presence of KCl, KNO3, or bis-tris-propane (BTP)-Cl. Using 36Cl-, we quantified Cl--transport activity by measuring the uptake of Cl- ions into the plasma membrane vesicles. The uptake depended on the potential difference across the membrane that was generated by H+-ATPase; it was enhanced by an inside-positive potential gradient. At 0.1 mM, NIF completely blocked the voltage-dependent Cl--transport activity. From these properties of the Cl- transporter and the inhibition of H+transport activity by NIF, we suggest that H+-transport activity across the plasma membrane might be modulated by the transport of anions via a NIF- sensitive anion-permeable transporter that acts to collapse the inside- positive potential generated by H+-ATPase.

KW - Cl uptake

KW - H-transport activity

KW - Hordeum vulgare L.

KW - Niflumic acid

KW - Plasma membrane

UR - http://www.scopus.com/inward/record.url?scp=0030460614&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030460614&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0030460614

VL - 37

SP - 949

EP - 956

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 7

ER -