Characterization of amyloid β fibrils with an aqueous two-phase system: Implications of fibril formation

Toshinori Shimanouchi, Naoya Shimauchi, Keiichi Nishiyama, Huong Thi Vu, Hisashi Yagi, Yuji Goto, Hiroshi Umakoshi, Ryoichi Kuboi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The pathological process of Alzheimer's disease is closely related to amyloid fibril formation by the causative protein, amyloid β (Aβ). The growth behavior of Aβ fibrils is predominated by the seeds-monomeric Aβ interaction. In this study, the local hydrophobicity of seeds of Aβ fibrils was investigated by the aqueous two-phase partitioning method to evaluate the hydrophobic interaction between the seed-monomeric Aβ. The seeds showed a high local hydrophobicity relative to the monomer and fibrils. From the fibril growth experiment and the additive effect of Triton X-100, we could demonstrate the contribution of the hydrophobic seeds-monomer interaction to fibril formation.

Original languageEnglish
Pages (from-to)121-128
Number of pages8
JournalSolvent Extraction Research and Development
Volume17
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Amyloid
Seed
Hydrophobicity
Monomers
Serum Amyloid A Protein
Octoxynol
Experiments

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Characterization of amyloid β fibrils with an aqueous two-phase system : Implications of fibril formation. / Shimanouchi, Toshinori; Shimauchi, Naoya; Nishiyama, Keiichi; Vu, Huong Thi; Yagi, Hisashi; Goto, Yuji; Umakoshi, Hiroshi; Kuboi, Ryoichi.

In: Solvent Extraction Research and Development, Vol. 17, 2010, p. 121-128.

Research output: Contribution to journalArticle

Shimanouchi, T, Shimauchi, N, Nishiyama, K, Vu, HT, Yagi, H, Goto, Y, Umakoshi, H & Kuboi, R 2010, 'Characterization of amyloid β fibrils with an aqueous two-phase system: Implications of fibril formation', Solvent Extraction Research and Development, vol. 17, pp. 121-128.
Shimanouchi, Toshinori ; Shimauchi, Naoya ; Nishiyama, Keiichi ; Vu, Huong Thi ; Yagi, Hisashi ; Goto, Yuji ; Umakoshi, Hiroshi ; Kuboi, Ryoichi. / Characterization of amyloid β fibrils with an aqueous two-phase system : Implications of fibril formation. In: Solvent Extraction Research and Development. 2010 ; Vol. 17. pp. 121-128.
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