Characterization of alternatively spliced transcripts encoding heat shock transcription factor in cultured cells of the cabbage armyworm, Mamestra brassicae

Shoji Sonoda, Hisaaki Tsumuki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A gene encoding heat shock transcription factor (HSF) was cloned and sequenced from cultured cells of the cabbage armyworm, Mamestra brassicae. The cDNA potentially encoded a 699-aa protein, with a calculated molecular weight of 77.8 kDa. Deduced amino acid identities to HSFs from Aedes aegypti and Drosophila melanogaster were 36 and 34%, respectively. Analysis of the genomic DNA revealed eight exons and three optional exons: a, b, and c. Exon a contained a premature in-frame stop codon that would generate a truncated protein. When the cells were exposed to high temperature or cadmium, no particular alternative transcripts showed significant up- or down-regulated expression relative to the total amount of the transcripts. These results suggest that alternative splicing may not be a principal mechanism for regulation of M. brassicae HSF gene expression in response to heat shock and cadmium.

Original languageEnglish
Pages (from-to)49-60
Number of pages12
JournalArchives of Insect Biochemistry and Physiology
Volume73
Issue number1
DOIs
Publication statusPublished - Jan 2010

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Keywords

  • Alternative splicing
  • Cadmium
  • Heat shock transcription factor
  • Mamestra brassicae

ASJC Scopus subject areas

  • Insect Science
  • Physiology
  • Biochemistry

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