Characterization of alternatively spliced transcripts encoding heat shock transcription factor in cultured cells of the cabbage armyworm, Mamestra brassicae

Shoji Sonoda, Hisaaki Tsumuki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A gene encoding heat shock transcription factor (HSF) was cloned and sequenced from cultured cells of the cabbage armyworm, Mamestra brassicae. The cDNA potentially encoded a 699-aa protein, with a calculated molecular weight of 77.8 kDa. Deduced amino acid identities to HSFs from Aedes aegypti and Drosophila melanogaster were 36 and 34%, respectively. Analysis of the genomic DNA revealed eight exons and three optional exons: a, b, and c. Exon a contained a premature in-frame stop codon that would generate a truncated protein. When the cells were exposed to high temperature or cadmium, no particular alternative transcripts showed significant up- or down-regulated expression relative to the total amount of the transcripts. These results suggest that alternative splicing may not be a principal mechanism for regulation of M. brassicae HSF gene expression in response to heat shock and cadmium.

Original languageEnglish
Pages (from-to)49-60
Number of pages12
JournalArchives of Insect Biochemistry and Physiology
Volume73
Issue number1
DOIs
Publication statusPublished - Jan 2010

Fingerprint

Mamestra brassicae
Brassica
alternative splicing
cabbage
cultured cells
exons
heat stress
Exons
Cultured Cells
transcription factors
Cells
Cadmium
cadmium
Heat-Shock Response
Gene encoding
Terminator Codon
Aedes
stop codon
Alternative Splicing
Aedes aegypti

Keywords

  • Alternative splicing
  • Cadmium
  • Heat shock transcription factor
  • Mamestra brassicae

ASJC Scopus subject areas

  • Insect Science
  • Physiology
  • Biochemistry

Cite this

@article{5e88e37e66f54207b8bdb560d52cdbf0,
title = "Characterization of alternatively spliced transcripts encoding heat shock transcription factor in cultured cells of the cabbage armyworm, Mamestra brassicae",
abstract = "A gene encoding heat shock transcription factor (HSF) was cloned and sequenced from cultured cells of the cabbage armyworm, Mamestra brassicae. The cDNA potentially encoded a 699-aa protein, with a calculated molecular weight of 77.8 kDa. Deduced amino acid identities to HSFs from Aedes aegypti and Drosophila melanogaster were 36 and 34{\%}, respectively. Analysis of the genomic DNA revealed eight exons and three optional exons: a, b, and c. Exon a contained a premature in-frame stop codon that would generate a truncated protein. When the cells were exposed to high temperature or cadmium, no particular alternative transcripts showed significant up- or down-regulated expression relative to the total amount of the transcripts. These results suggest that alternative splicing may not be a principal mechanism for regulation of M. brassicae HSF gene expression in response to heat shock and cadmium.",
keywords = "Alternative splicing, Cadmium, Heat shock transcription factor, Mamestra brassicae",
author = "Shoji Sonoda and Hisaaki Tsumuki",
year = "2010",
month = "1",
doi = "10.1002/arch.20339",
language = "English",
volume = "73",
pages = "49--60",
journal = "Archives of Insect Biochemistry and Physiology",
issn = "0739-4462",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - Characterization of alternatively spliced transcripts encoding heat shock transcription factor in cultured cells of the cabbage armyworm, Mamestra brassicae

AU - Sonoda, Shoji

AU - Tsumuki, Hisaaki

PY - 2010/1

Y1 - 2010/1

N2 - A gene encoding heat shock transcription factor (HSF) was cloned and sequenced from cultured cells of the cabbage armyworm, Mamestra brassicae. The cDNA potentially encoded a 699-aa protein, with a calculated molecular weight of 77.8 kDa. Deduced amino acid identities to HSFs from Aedes aegypti and Drosophila melanogaster were 36 and 34%, respectively. Analysis of the genomic DNA revealed eight exons and three optional exons: a, b, and c. Exon a contained a premature in-frame stop codon that would generate a truncated protein. When the cells were exposed to high temperature or cadmium, no particular alternative transcripts showed significant up- or down-regulated expression relative to the total amount of the transcripts. These results suggest that alternative splicing may not be a principal mechanism for regulation of M. brassicae HSF gene expression in response to heat shock and cadmium.

AB - A gene encoding heat shock transcription factor (HSF) was cloned and sequenced from cultured cells of the cabbage armyworm, Mamestra brassicae. The cDNA potentially encoded a 699-aa protein, with a calculated molecular weight of 77.8 kDa. Deduced amino acid identities to HSFs from Aedes aegypti and Drosophila melanogaster were 36 and 34%, respectively. Analysis of the genomic DNA revealed eight exons and three optional exons: a, b, and c. Exon a contained a premature in-frame stop codon that would generate a truncated protein. When the cells were exposed to high temperature or cadmium, no particular alternative transcripts showed significant up- or down-regulated expression relative to the total amount of the transcripts. These results suggest that alternative splicing may not be a principal mechanism for regulation of M. brassicae HSF gene expression in response to heat shock and cadmium.

KW - Alternative splicing

KW - Cadmium

KW - Heat shock transcription factor

KW - Mamestra brassicae

UR - http://www.scopus.com/inward/record.url?scp=72249112193&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72249112193&partnerID=8YFLogxK

U2 - 10.1002/arch.20339

DO - 10.1002/arch.20339

M3 - Article

VL - 73

SP - 49

EP - 60

JO - Archives of Insect Biochemistry and Physiology

JF - Archives of Insect Biochemistry and Physiology

SN - 0739-4462

IS - 1

ER -