Characterization of allergen-specific monocyte-derived dendritic cells generated from monocytes by a single-step procedure: Effect on naïve and memory T cells

T. Takishita, Mitsuhiro Okano, K. Takahashi, Tadashi Yoshino, Y. Sugata, H. Hattori, S. Ohuchi, T. Ogawa, Kazunori Nishizaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Dendritic cells are one of the most potent antigen-presenting cells and when pulsed with allergen can modulate allergen-specific T-cell responses. We sought to establish a single-step method by which to generate allergen-specific monocyte-derived dendritic cells (MoDCs). Methods: Dermatophagoides farinae (Df)-prepulsed MoDCs were generated from monocytes by culturing with Df in the presence interleukin (IL)-4, granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumour necrosis factor (TNF)-α simultaneously. Df-prepulsed MoDC were incubated with autologous naïve and memory T cells in the absence of recall antigen, then proliferation and cytokine production by T cells was determined. Results: Generation of allergen-prepulsed MoDCs was confirmed by examining expression of surface molecules. Df-prepulsed MoDC selectively induced proliferation of Df-specific T cells in the absence of recall antigen. Under these conditions, Df-prepulsed MoDCs augmented but did not alter the cytokine production profile. In addition, Df-prepulsed MoDCs activated naïve T cells leading to proliferation and selective production of IFN-γ in allergic patients but not in healthy subjects. Conclusions: These results suggest that Df-prepulsed MoDC generated from monocytes by a simple single-step manipulation can induce Df-specific cellular responses from both naïve and memory T cells in the absence of recall antigen, and these cells potentially can be utilized as immune adjuvants in allergen-specific immunotherapy.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume60
Issue number2
DOIs
Publication statusPublished - Feb 2005

Fingerprint

Dermatophagoides farinae
Allergens
Dendritic Cells
Monocytes
T-Lymphocytes
Antigens
Cytokines
Immunologic Desensitization
Antigen-Presenting Cells
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-4
Healthy Volunteers
Tumor Necrosis Factor-alpha

Keywords

  • Allergen
  • Cytokine
  • Dendritic cells
  • Memory
  • Naïve
  • Rhinitis
  • T cell

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of allergen-specific monocyte-derived dendritic cells generated from monocytes by a single-step procedure : Effect on naïve and memory T cells. / Takishita, T.; Okano, Mitsuhiro; Takahashi, K.; Yoshino, Tadashi; Sugata, Y.; Hattori, H.; Ohuchi, S.; Ogawa, T.; Nishizaki, Kazunori.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 60, No. 2, 02.2005, p. 211-217.

Research output: Contribution to journalArticle

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abstract = "Background: Dendritic cells are one of the most potent antigen-presenting cells and when pulsed with allergen can modulate allergen-specific T-cell responses. We sought to establish a single-step method by which to generate allergen-specific monocyte-derived dendritic cells (MoDCs). Methods: Dermatophagoides farinae (Df)-prepulsed MoDCs were generated from monocytes by culturing with Df in the presence interleukin (IL)-4, granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumour necrosis factor (TNF)-α simultaneously. Df-prepulsed MoDC were incubated with autologous na{\"i}ve and memory T cells in the absence of recall antigen, then proliferation and cytokine production by T cells was determined. Results: Generation of allergen-prepulsed MoDCs was confirmed by examining expression of surface molecules. Df-prepulsed MoDC selectively induced proliferation of Df-specific T cells in the absence of recall antigen. Under these conditions, Df-prepulsed MoDCs augmented but did not alter the cytokine production profile. In addition, Df-prepulsed MoDCs activated na{\"i}ve T cells leading to proliferation and selective production of IFN-γ in allergic patients but not in healthy subjects. Conclusions: These results suggest that Df-prepulsed MoDC generated from monocytes by a simple single-step manipulation can induce Df-specific cellular responses from both na{\"i}ve and memory T cells in the absence of recall antigen, and these cells potentially can be utilized as immune adjuvants in allergen-specific immunotherapy.",
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AU - Yoshino, Tadashi

AU - Sugata, Y.

AU - Hattori, H.

AU - Ohuchi, S.

AU - Ogawa, T.

AU - Nishizaki, Kazunori

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KW - Allergen

KW - Cytokine

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