Abstract
Decreased acetylcholinesterase (AChE) sensitivity and metabolic detoxification mediated by glutathione S-transferases (GSTs) were examined for their involvement in resistance to acephate in the diamondback moth, Plutella xylostella. The resistant strain showed 47.5-fold higher acephate resistance than the susceptible strain had. However, the resistant strain was only 2.3-fold more resistant to prothiofos than the susceptible strain. The resistant strain included insects having the A298S and G324A mutations in AChE1, which are reportedly involved in prothiofos resistance in P. xylostella, showing reduced AChE sensitivity to inhibition by methamidophos, suggesting that decreased AChE1 sensitivity is one factor conferring acephate resistance. However, allele frequencies at both mutation sites in the resistant strain were low (only 26%). These results suggest that other factors such as GSTs are involved in acephate resistance. Expression of GST genes available in P. xylostella to date was examined using the resistant and susceptible strains, revealing no significant correlation between the expression and resistance levels.
| Original language | English |
|---|---|
| Pages (from-to) | 121-127 |
| Number of pages | 7 |
| Journal | Pesticide Biochemistry and Physiology |
| Volume | 98 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Sep 2010 |
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Keywords
- Acephate
- Acetylcholinesterase
- Diamondback moth
- Glutathione S-transferase
- Insecticide resistance
- Organophosphate
ASJC Scopus subject areas
- Agronomy and Crop Science
- Health, Toxicology and Mutagenesis
Cite this
Characterization of acephate resistance in the diamondback moth Plutella xylostella. / Sonoda, Shoji; Igaki, Chikako.
In: Pesticide Biochemistry and Physiology, Vol. 98, No. 1, 09.2010, p. 121-127.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of acephate resistance in the diamondback moth Plutella xylostella
AU - Sonoda, Shoji
AU - Igaki, Chikako
PY - 2010/9
Y1 - 2010/9
N2 - Decreased acetylcholinesterase (AChE) sensitivity and metabolic detoxification mediated by glutathione S-transferases (GSTs) were examined for their involvement in resistance to acephate in the diamondback moth, Plutella xylostella. The resistant strain showed 47.5-fold higher acephate resistance than the susceptible strain had. However, the resistant strain was only 2.3-fold more resistant to prothiofos than the susceptible strain. The resistant strain included insects having the A298S and G324A mutations in AChE1, which are reportedly involved in prothiofos resistance in P. xylostella, showing reduced AChE sensitivity to inhibition by methamidophos, suggesting that decreased AChE1 sensitivity is one factor conferring acephate resistance. However, allele frequencies at both mutation sites in the resistant strain were low (only 26%). These results suggest that other factors such as GSTs are involved in acephate resistance. Expression of GST genes available in P. xylostella to date was examined using the resistant and susceptible strains, revealing no significant correlation between the expression and resistance levels.
AB - Decreased acetylcholinesterase (AChE) sensitivity and metabolic detoxification mediated by glutathione S-transferases (GSTs) were examined for their involvement in resistance to acephate in the diamondback moth, Plutella xylostella. The resistant strain showed 47.5-fold higher acephate resistance than the susceptible strain had. However, the resistant strain was only 2.3-fold more resistant to prothiofos than the susceptible strain. The resistant strain included insects having the A298S and G324A mutations in AChE1, which are reportedly involved in prothiofos resistance in P. xylostella, showing reduced AChE sensitivity to inhibition by methamidophos, suggesting that decreased AChE1 sensitivity is one factor conferring acephate resistance. However, allele frequencies at both mutation sites in the resistant strain were low (only 26%). These results suggest that other factors such as GSTs are involved in acephate resistance. Expression of GST genes available in P. xylostella to date was examined using the resistant and susceptible strains, revealing no significant correlation between the expression and resistance levels.
KW - Acephate
KW - Acetylcholinesterase
KW - Diamondback moth
KW - Glutathione S-transferase
KW - Insecticide resistance
KW - Organophosphate
UR - http://www.scopus.com/inward/record.url?scp=77954953411&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954953411&partnerID=8YFLogxK
U2 - 10.1016/j.pestbp.2010.05.010
DO - 10.1016/j.pestbp.2010.05.010
M3 - Article
VL - 98
SP - 121
EP - 127
JO - Pesticide Biochemistry and Physiology
JF - Pesticide Biochemistry and Physiology
SN - 0048-3575
IS - 1
ER -