Characterization of acephate resistance in the diamondback moth Plutella xylostella

Shoji Sonoda, Chikako Igaki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Decreased acetylcholinesterase (AChE) sensitivity and metabolic detoxification mediated by glutathione S-transferases (GSTs) were examined for their involvement in resistance to acephate in the diamondback moth, Plutella xylostella. The resistant strain showed 47.5-fold higher acephate resistance than the susceptible strain had. However, the resistant strain was only 2.3-fold more resistant to prothiofos than the susceptible strain. The resistant strain included insects having the A298S and G324A mutations in AChE1, which are reportedly involved in prothiofos resistance in P. xylostella, showing reduced AChE sensitivity to inhibition by methamidophos, suggesting that decreased AChE1 sensitivity is one factor conferring acephate resistance. However, allele frequencies at both mutation sites in the resistant strain were low (only 26%). These results suggest that other factors such as GSTs are involved in acephate resistance. Expression of GST genes available in P. xylostella to date was examined using the resistant and susceptible strains, revealing no significant correlation between the expression and resistance levels.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalPesticide Biochemistry and Physiology
Volume98
Issue number1
DOIs
Publication statusPublished - Sep 2010

Fingerprint

acephate
Moths
Plutella xylostella
Glutathione Transferase
Acetylcholinesterase
glutathione transferase
Mutation
acetylcholinesterase
Gene Frequency
Insects
metabolic detoxification
methamidophos
mutation
gene frequency

Keywords

  • Acephate
  • Acetylcholinesterase
  • Diamondback moth
  • Glutathione S-transferase
  • Insecticide resistance
  • Organophosphate

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

Cite this

Characterization of acephate resistance in the diamondback moth Plutella xylostella. / Sonoda, Shoji; Igaki, Chikako.

In: Pesticide Biochemistry and Physiology, Vol. 98, No. 1, 09.2010, p. 121-127.

Research output: Contribution to journalArticle

@article{23a33a7b0d274aa39c30d7a3a87e7aab,
title = "Characterization of acephate resistance in the diamondback moth Plutella xylostella",
abstract = "Decreased acetylcholinesterase (AChE) sensitivity and metabolic detoxification mediated by glutathione S-transferases (GSTs) were examined for their involvement in resistance to acephate in the diamondback moth, Plutella xylostella. The resistant strain showed 47.5-fold higher acephate resistance than the susceptible strain had. However, the resistant strain was only 2.3-fold more resistant to prothiofos than the susceptible strain. The resistant strain included insects having the A298S and G324A mutations in AChE1, which are reportedly involved in prothiofos resistance in P. xylostella, showing reduced AChE sensitivity to inhibition by methamidophos, suggesting that decreased AChE1 sensitivity is one factor conferring acephate resistance. However, allele frequencies at both mutation sites in the resistant strain were low (only 26{\%}). These results suggest that other factors such as GSTs are involved in acephate resistance. Expression of GST genes available in P. xylostella to date was examined using the resistant and susceptible strains, revealing no significant correlation between the expression and resistance levels.",
keywords = "Acephate, Acetylcholinesterase, Diamondback moth, Glutathione S-transferase, Insecticide resistance, Organophosphate",
author = "Shoji Sonoda and Chikako Igaki",
year = "2010",
month = "9",
doi = "10.1016/j.pestbp.2010.05.010",
language = "English",
volume = "98",
pages = "121--127",
journal = "Pesticide Biochemistry and Physiology",
issn = "0048-3575",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Characterization of acephate resistance in the diamondback moth Plutella xylostella

AU - Sonoda, Shoji

AU - Igaki, Chikako

PY - 2010/9

Y1 - 2010/9

N2 - Decreased acetylcholinesterase (AChE) sensitivity and metabolic detoxification mediated by glutathione S-transferases (GSTs) were examined for their involvement in resistance to acephate in the diamondback moth, Plutella xylostella. The resistant strain showed 47.5-fold higher acephate resistance than the susceptible strain had. However, the resistant strain was only 2.3-fold more resistant to prothiofos than the susceptible strain. The resistant strain included insects having the A298S and G324A mutations in AChE1, which are reportedly involved in prothiofos resistance in P. xylostella, showing reduced AChE sensitivity to inhibition by methamidophos, suggesting that decreased AChE1 sensitivity is one factor conferring acephate resistance. However, allele frequencies at both mutation sites in the resistant strain were low (only 26%). These results suggest that other factors such as GSTs are involved in acephate resistance. Expression of GST genes available in P. xylostella to date was examined using the resistant and susceptible strains, revealing no significant correlation between the expression and resistance levels.

AB - Decreased acetylcholinesterase (AChE) sensitivity and metabolic detoxification mediated by glutathione S-transferases (GSTs) were examined for their involvement in resistance to acephate in the diamondback moth, Plutella xylostella. The resistant strain showed 47.5-fold higher acephate resistance than the susceptible strain had. However, the resistant strain was only 2.3-fold more resistant to prothiofos than the susceptible strain. The resistant strain included insects having the A298S and G324A mutations in AChE1, which are reportedly involved in prothiofos resistance in P. xylostella, showing reduced AChE sensitivity to inhibition by methamidophos, suggesting that decreased AChE1 sensitivity is one factor conferring acephate resistance. However, allele frequencies at both mutation sites in the resistant strain were low (only 26%). These results suggest that other factors such as GSTs are involved in acephate resistance. Expression of GST genes available in P. xylostella to date was examined using the resistant and susceptible strains, revealing no significant correlation between the expression and resistance levels.

KW - Acephate

KW - Acetylcholinesterase

KW - Diamondback moth

KW - Glutathione S-transferase

KW - Insecticide resistance

KW - Organophosphate

UR - http://www.scopus.com/inward/record.url?scp=77954953411&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954953411&partnerID=8YFLogxK

U2 - 10.1016/j.pestbp.2010.05.010

DO - 10.1016/j.pestbp.2010.05.010

M3 - Article

AN - SCOPUS:77954953411

VL - 98

SP - 121

EP - 127

JO - Pesticide Biochemistry and Physiology

JF - Pesticide Biochemistry and Physiology

SN - 0048-3575

IS - 1

ER -