Characterization of a rice variety with high hydraulic conductance and identification of the chromosome region responsible using chromosome segment substitution lines

Shunsuke Adachi, Yukiko Tsuru, Motohiko Kondo, Toshio Yamamoto, Yumiko Arai-Sanoh, Tsuyu Ando, Taiichiro Ookawa, Masahiro Yano, Tadashi Hirasawa

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background and Aims: The rate of photosynthesis in paddy rice often decreases at noon on sunny days because of water stress, even under submerged conditions. Maintenance of higher rates of photosynthesis during the day might improve both yield and dry matter production in paddy rice. A high-yielding indica variety, 'Habataki', maintains a high rate of leaf photosynthesis during the daytime because of the higher hydraulic conductance from roots to leaves than in the standard japonica variety 'Sasanishiki'. This research was conducted to characterize the trait responsible for the higher hydraulic conductance in 'Habataki' and identified a chromosome region for the high hydraulic conductance. Methods: Hydraulic conductance to passive water transport and to osmotic water transport was determined for plants under intense transpiration and for plants without transpiration, respectively. The varietal difference in hydraulic conductance was examined with respect to root surface area and hydraulic conductivity (hydraulic conductance per root surface area, L p). To identify the chromosome region responsible for higher hydraulic conductance, chromosome segment substitution lines (CSSLs) derived from a cross between 'Sasanishiki' and 'Habataki' were used. Key Results: The significantly higher hydraulic conductance resulted from the larger root surface area not from Lp in 'Habataki'. A chromosome region associated with the elevated hydraulic conductance was detected between RM3916 and RM2431 on the long arm of chromosome 4. The CSSL, in which this region was substituted with the 'Habataki' chromosome segment in the 'Sasanishiki' background, had a larger root mass than 'Sasanishiki'. Conclusions: The trait for increasing plant hydraulic conductance and, therefore, maintaining the higher rate of leaf photosynthesis under the conditions of intense transpiration in 'Habataki' was identified, and it was estimated that there is at least one chromosome region for the trait located on chromosome 4.

Original languageEnglish
Pages (from-to)803-811
Number of pages9
JournalAnnals of Botany
Volume106
Issue number5
DOIs
Publication statusPublished - Nov 1 2010
Externally publishedYes

Fingerprint

substitution lines
fluid mechanics
chromosomes
rice
photosynthesis
transpiration
surface area
leaves
hydraulic conductivity
dry matter accumulation
water stress
water

Keywords

  • Chromosome segment substitution lines
  • diffusive conductance
  • hydraulic conductance
  • Oryza sativa
  • photosynthetic rate
  • quantitative trait locus
  • rice
  • root hydraulic conductivity

ASJC Scopus subject areas

  • Plant Science

Cite this

Characterization of a rice variety with high hydraulic conductance and identification of the chromosome region responsible using chromosome segment substitution lines. / Adachi, Shunsuke; Tsuru, Yukiko; Kondo, Motohiko; Yamamoto, Toshio; Arai-Sanoh, Yumiko; Ando, Tsuyu; Ookawa, Taiichiro; Yano, Masahiro; Hirasawa, Tadashi.

In: Annals of Botany, Vol. 106, No. 5, 01.11.2010, p. 803-811.

Research output: Contribution to journalArticle

Adachi, Shunsuke ; Tsuru, Yukiko ; Kondo, Motohiko ; Yamamoto, Toshio ; Arai-Sanoh, Yumiko ; Ando, Tsuyu ; Ookawa, Taiichiro ; Yano, Masahiro ; Hirasawa, Tadashi. / Characterization of a rice variety with high hydraulic conductance and identification of the chromosome region responsible using chromosome segment substitution lines. In: Annals of Botany. 2010 ; Vol. 106, No. 5. pp. 803-811.
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abstract = "Background and Aims: The rate of photosynthesis in paddy rice often decreases at noon on sunny days because of water stress, even under submerged conditions. Maintenance of higher rates of photosynthesis during the day might improve both yield and dry matter production in paddy rice. A high-yielding indica variety, 'Habataki', maintains a high rate of leaf photosynthesis during the daytime because of the higher hydraulic conductance from roots to leaves than in the standard japonica variety 'Sasanishiki'. This research was conducted to characterize the trait responsible for the higher hydraulic conductance in 'Habataki' and identified a chromosome region for the high hydraulic conductance. Methods: Hydraulic conductance to passive water transport and to osmotic water transport was determined for plants under intense transpiration and for plants without transpiration, respectively. The varietal difference in hydraulic conductance was examined with respect to root surface area and hydraulic conductivity (hydraulic conductance per root surface area, L p). To identify the chromosome region responsible for higher hydraulic conductance, chromosome segment substitution lines (CSSLs) derived from a cross between 'Sasanishiki' and 'Habataki' were used. Key Results: The significantly higher hydraulic conductance resulted from the larger root surface area not from Lp in 'Habataki'. A chromosome region associated with the elevated hydraulic conductance was detected between RM3916 and RM2431 on the long arm of chromosome 4. The CSSL, in which this region was substituted with the 'Habataki' chromosome segment in the 'Sasanishiki' background, had a larger root mass than 'Sasanishiki'. Conclusions: The trait for increasing plant hydraulic conductance and, therefore, maintaining the higher rate of leaf photosynthesis under the conditions of intense transpiration in 'Habataki' was identified, and it was estimated that there is at least one chromosome region for the trait located on chromosome 4.",
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AU - Adachi, Shunsuke

AU - Tsuru, Yukiko

AU - Kondo, Motohiko

AU - Yamamoto, Toshio

AU - Arai-Sanoh, Yumiko

AU - Ando, Tsuyu

AU - Ookawa, Taiichiro

AU - Yano, Masahiro

AU - Hirasawa, Tadashi

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AB - Background and Aims: The rate of photosynthesis in paddy rice often decreases at noon on sunny days because of water stress, even under submerged conditions. Maintenance of higher rates of photosynthesis during the day might improve both yield and dry matter production in paddy rice. A high-yielding indica variety, 'Habataki', maintains a high rate of leaf photosynthesis during the daytime because of the higher hydraulic conductance from roots to leaves than in the standard japonica variety 'Sasanishiki'. This research was conducted to characterize the trait responsible for the higher hydraulic conductance in 'Habataki' and identified a chromosome region for the high hydraulic conductance. Methods: Hydraulic conductance to passive water transport and to osmotic water transport was determined for plants under intense transpiration and for plants without transpiration, respectively. The varietal difference in hydraulic conductance was examined with respect to root surface area and hydraulic conductivity (hydraulic conductance per root surface area, L p). To identify the chromosome region responsible for higher hydraulic conductance, chromosome segment substitution lines (CSSLs) derived from a cross between 'Sasanishiki' and 'Habataki' were used. Key Results: The significantly higher hydraulic conductance resulted from the larger root surface area not from Lp in 'Habataki'. A chromosome region associated with the elevated hydraulic conductance was detected between RM3916 and RM2431 on the long arm of chromosome 4. The CSSL, in which this region was substituted with the 'Habataki' chromosome segment in the 'Sasanishiki' background, had a larger root mass than 'Sasanishiki'. Conclusions: The trait for increasing plant hydraulic conductance and, therefore, maintaining the higher rate of leaf photosynthesis under the conditions of intense transpiration in 'Habataki' was identified, and it was estimated that there is at least one chromosome region for the trait located on chromosome 4.

KW - Chromosome segment substitution lines

KW - diffusive conductance

KW - hydraulic conductance

KW - Oryza sativa

KW - photosynthetic rate

KW - quantitative trait locus

KW - rice

KW - root hydraulic conductivity

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