Characterization of a novel human natural killer-cell line (NK-YS) established from natural killer cell lymphoma/leukemia associated with Epstein-Barr virus infection

Junjiro Tsuchiyama, Tadashi Yoshino, Masaharu Mori, Eisaku Kondoh, Takashi Oka, Tadaatsu Akagi, Akio Hiraki, Hiroyuki Nakayama, Akira Shibuya, Yuxiang Ma, Teruyuki Kawabata, Shigeru Okada, Mine Harada

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

A novel cell line was established from a patient with a leukemic-state nasal angiocentric natural killer (NK) cell lymphoma with systemic skin infiltration. The morphology of the leukemic cells was large-granular- lymphocyte (LGL), and their immunophenotype was CD2+, CD3-, CD5+, CD7+, CD16-, CD56+, and CD57-. The presence of Epstein-Barr viral (EBV) genome was shown in specimens from the patient's nose, skin, and peripheral blood by in situ hybridization using an EBV-encoded small RNA-1 probe or by Southern blotting using a terminal-repeat probe of the EBV genome. Leukemic cells were cocultured with a mouse stromal cell line (SPY3-2) in the presence of 100 U/mL recombinant human interleukin-2 and a novel stromal cell-independent cell line, NK-YS, was established. The NK-YS cells showed LGL morphology and expressed surface CD2, CD5, CD7, CD25, CD56, and CD95. The NK-YS cells retained cytotoxicity against K562 and Jurkat cells. A Southern blotting using a terminal-repeat probe of EBV showed that NK-YS and fresh leukemic cells had a clonal EBV genome, whereas the T-cell receptor β and γ chain genes of NK-YS were not rearranged. In an immunocytochemical analysis, the NK-YS cells showed a type-II latent infection of EBV. The NK-YS cells preserved the original characteristics of NK cell lymphoma/leukemia and will be a useful tool for the study of biological characteristics of EBV- associated nasal angiocentric NK cell lymphoma/leukemia.

Original languageEnglish
Pages (from-to)1374-1383
Number of pages10
JournalBlood
Volume92
Issue number4
Publication statusPublished - Aug 15 1998
Externally publishedYes

Fingerprint

Epstein-Barr Virus Infections
Viruses
Natural Killer Cells
Lymphoma
Leukemia
Genes
Cells
Cell Line
Lymphocytes
Terminal Repeat Sequences
Viral Genome
Nose
Skin
Stromal Cells
Southern Blotting
Cytotoxicity
T-Cell Antigen Receptor
Infiltration
Interleukin-2
Blood

ASJC Scopus subject areas

  • Hematology

Cite this

Characterization of a novel human natural killer-cell line (NK-YS) established from natural killer cell lymphoma/leukemia associated with Epstein-Barr virus infection. / Tsuchiyama, Junjiro; Yoshino, Tadashi; Mori, Masaharu; Kondoh, Eisaku; Oka, Takashi; Akagi, Tadaatsu; Hiraki, Akio; Nakayama, Hiroyuki; Shibuya, Akira; Ma, Yuxiang; Kawabata, Teruyuki; Okada, Shigeru; Harada, Mine.

In: Blood, Vol. 92, No. 4, 15.08.1998, p. 1374-1383.

Research output: Contribution to journalArticle

Tsuchiyama, J, Yoshino, T, Mori, M, Kondoh, E, Oka, T, Akagi, T, Hiraki, A, Nakayama, H, Shibuya, A, Ma, Y, Kawabata, T, Okada, S & Harada, M 1998, 'Characterization of a novel human natural killer-cell line (NK-YS) established from natural killer cell lymphoma/leukemia associated with Epstein-Barr virus infection', Blood, vol. 92, no. 4, pp. 1374-1383.
Tsuchiyama, Junjiro ; Yoshino, Tadashi ; Mori, Masaharu ; Kondoh, Eisaku ; Oka, Takashi ; Akagi, Tadaatsu ; Hiraki, Akio ; Nakayama, Hiroyuki ; Shibuya, Akira ; Ma, Yuxiang ; Kawabata, Teruyuki ; Okada, Shigeru ; Harada, Mine. / Characterization of a novel human natural killer-cell line (NK-YS) established from natural killer cell lymphoma/leukemia associated with Epstein-Barr virus infection. In: Blood. 1998 ; Vol. 92, No. 4. pp. 1374-1383.
@article{02b81d7643d44f0599527b5696876c61,
title = "Characterization of a novel human natural killer-cell line (NK-YS) established from natural killer cell lymphoma/leukemia associated with Epstein-Barr virus infection",
abstract = "A novel cell line was established from a patient with a leukemic-state nasal angiocentric natural killer (NK) cell lymphoma with systemic skin infiltration. The morphology of the leukemic cells was large-granular- lymphocyte (LGL), and their immunophenotype was CD2+, CD3-, CD5+, CD7+, CD16-, CD56+, and CD57-. The presence of Epstein-Barr viral (EBV) genome was shown in specimens from the patient's nose, skin, and peripheral blood by in situ hybridization using an EBV-encoded small RNA-1 probe or by Southern blotting using a terminal-repeat probe of the EBV genome. Leukemic cells were cocultured with a mouse stromal cell line (SPY3-2) in the presence of 100 U/mL recombinant human interleukin-2 and a novel stromal cell-independent cell line, NK-YS, was established. The NK-YS cells showed LGL morphology and expressed surface CD2, CD5, CD7, CD25, CD56, and CD95. The NK-YS cells retained cytotoxicity against K562 and Jurkat cells. A Southern blotting using a terminal-repeat probe of EBV showed that NK-YS and fresh leukemic cells had a clonal EBV genome, whereas the T-cell receptor β and γ chain genes of NK-YS were not rearranged. In an immunocytochemical analysis, the NK-YS cells showed a type-II latent infection of EBV. The NK-YS cells preserved the original characteristics of NK cell lymphoma/leukemia and will be a useful tool for the study of biological characteristics of EBV- associated nasal angiocentric NK cell lymphoma/leukemia.",
author = "Junjiro Tsuchiyama and Tadashi Yoshino and Masaharu Mori and Eisaku Kondoh and Takashi Oka and Tadaatsu Akagi and Akio Hiraki and Hiroyuki Nakayama and Akira Shibuya and Yuxiang Ma and Teruyuki Kawabata and Shigeru Okada and Mine Harada",
year = "1998",
month = "8",
day = "15",
language = "English",
volume = "92",
pages = "1374--1383",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "4",

}

TY - JOUR

T1 - Characterization of a novel human natural killer-cell line (NK-YS) established from natural killer cell lymphoma/leukemia associated with Epstein-Barr virus infection

AU - Tsuchiyama, Junjiro

AU - Yoshino, Tadashi

AU - Mori, Masaharu

AU - Kondoh, Eisaku

AU - Oka, Takashi

AU - Akagi, Tadaatsu

AU - Hiraki, Akio

AU - Nakayama, Hiroyuki

AU - Shibuya, Akira

AU - Ma, Yuxiang

AU - Kawabata, Teruyuki

AU - Okada, Shigeru

AU - Harada, Mine

PY - 1998/8/15

Y1 - 1998/8/15

N2 - A novel cell line was established from a patient with a leukemic-state nasal angiocentric natural killer (NK) cell lymphoma with systemic skin infiltration. The morphology of the leukemic cells was large-granular- lymphocyte (LGL), and their immunophenotype was CD2+, CD3-, CD5+, CD7+, CD16-, CD56+, and CD57-. The presence of Epstein-Barr viral (EBV) genome was shown in specimens from the patient's nose, skin, and peripheral blood by in situ hybridization using an EBV-encoded small RNA-1 probe or by Southern blotting using a terminal-repeat probe of the EBV genome. Leukemic cells were cocultured with a mouse stromal cell line (SPY3-2) in the presence of 100 U/mL recombinant human interleukin-2 and a novel stromal cell-independent cell line, NK-YS, was established. The NK-YS cells showed LGL morphology and expressed surface CD2, CD5, CD7, CD25, CD56, and CD95. The NK-YS cells retained cytotoxicity against K562 and Jurkat cells. A Southern blotting using a terminal-repeat probe of EBV showed that NK-YS and fresh leukemic cells had a clonal EBV genome, whereas the T-cell receptor β and γ chain genes of NK-YS were not rearranged. In an immunocytochemical analysis, the NK-YS cells showed a type-II latent infection of EBV. The NK-YS cells preserved the original characteristics of NK cell lymphoma/leukemia and will be a useful tool for the study of biological characteristics of EBV- associated nasal angiocentric NK cell lymphoma/leukemia.

AB - A novel cell line was established from a patient with a leukemic-state nasal angiocentric natural killer (NK) cell lymphoma with systemic skin infiltration. The morphology of the leukemic cells was large-granular- lymphocyte (LGL), and their immunophenotype was CD2+, CD3-, CD5+, CD7+, CD16-, CD56+, and CD57-. The presence of Epstein-Barr viral (EBV) genome was shown in specimens from the patient's nose, skin, and peripheral blood by in situ hybridization using an EBV-encoded small RNA-1 probe or by Southern blotting using a terminal-repeat probe of the EBV genome. Leukemic cells were cocultured with a mouse stromal cell line (SPY3-2) in the presence of 100 U/mL recombinant human interleukin-2 and a novel stromal cell-independent cell line, NK-YS, was established. The NK-YS cells showed LGL morphology and expressed surface CD2, CD5, CD7, CD25, CD56, and CD95. The NK-YS cells retained cytotoxicity against K562 and Jurkat cells. A Southern blotting using a terminal-repeat probe of EBV showed that NK-YS and fresh leukemic cells had a clonal EBV genome, whereas the T-cell receptor β and γ chain genes of NK-YS were not rearranged. In an immunocytochemical analysis, the NK-YS cells showed a type-II latent infection of EBV. The NK-YS cells preserved the original characteristics of NK cell lymphoma/leukemia and will be a useful tool for the study of biological characteristics of EBV- associated nasal angiocentric NK cell lymphoma/leukemia.

UR - http://www.scopus.com/inward/record.url?scp=0032529678&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032529678&partnerID=8YFLogxK

M3 - Article

VL - 92

SP - 1374

EP - 1383

JO - Blood

JF - Blood

SN - 0006-4971

IS - 4

ER -