Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum)

Hirohito Ogawa, Masahiro Kajihara, Naganori Nao, Asako Shigeno, Daisuke Fujikura, Bernard M Hang'ombe, Aaron S Mweene, Alisheke Mutemwa, David Squarre, Masao Yamada, Hideaki Higashi, Hirofumi Sawa, Ayato Takada

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bats are important reservoirs for emerging zoonotic viruses. For extensive surveys of potential pathogens in straw-colored fruit bats (Eidolon helvum) in Zambia, a total of 107 spleen samples of E. helvum in 2006 were inoculated onto Vero E6 cells. The cell culture inoculated with one of the samples (ZFB06-106) exhibited remarkable cytopathic changes. Based on the ultrastructural property in negative staining and cross-reactivity in immunofluorescence assays, the virus was suspected to be an adenovirus, and tentatively named E. helvum adenovirus 06-106 (EhAdV 06-106). Analysis of the full-length genome of 30,134 bp, determined by next-generation sequencing, showed the presence of 28 open reading frames. Phylogenetic analyses confirmed that EhAdV 06-106 represented a novel bat adenovirus species in the genus Mastadenovirus. The virus shared similar characteristics of low G + C contents with recently isolated members of species Bat mastadenoviruses E, F and G, from which EhAdV 06-106 diverged by more than 15% based on the distance matrix analysis of DNA polymerase amino acid sequences. According to the taxonomic criteria, we propose the tentative new species name "Bat mastadenovirus H". Because EhAdV 06-106 exhibited a wide in vitro cell tropism, the virus might have a potential risk as an emerging virus through cross-species transmission.

Original languageEnglish
JournalViruses
Volume9
Issue number12
DOIs
Publication statusPublished - Dec 4 2017
Externally publishedYes

Fingerprint

Adenoviridae
Mastadenovirus
Fruit
Viruses
Zambia
Negative Staining
Vero Cells
Tropism
Zoonoses
Base Composition
DNA-Directed DNA Polymerase
Open Reading Frames
Names
Fluorescent Antibody Technique
Amino Acid Sequence
Spleen
Cell Culture Techniques
Genome

Keywords

  • Adenovirus
  • Bat
  • Eidolon helvum
  • Zambia

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Ogawa, H., Kajihara, M., Nao, N., Shigeno, A., Fujikura, D., Hang'ombe, B. M., ... Takada, A. (2017). Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum). Viruses, 9(12). https://doi.org/10.3390/v9120371

Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum). / Ogawa, Hirohito; Kajihara, Masahiro; Nao, Naganori; Shigeno, Asako; Fujikura, Daisuke; Hang'ombe, Bernard M; Mweene, Aaron S; Mutemwa, Alisheke; Squarre, David; Yamada, Masao; Higashi, Hideaki; Sawa, Hirofumi; Takada, Ayato.

In: Viruses, Vol. 9, No. 12, 04.12.2017.

Research output: Contribution to journalArticle

Ogawa, H, Kajihara, M, Nao, N, Shigeno, A, Fujikura, D, Hang'ombe, BM, Mweene, AS, Mutemwa, A, Squarre, D, Yamada, M, Higashi, H, Sawa, H & Takada, A 2017, 'Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum)', Viruses, vol. 9, no. 12. https://doi.org/10.3390/v9120371
Ogawa, Hirohito ; Kajihara, Masahiro ; Nao, Naganori ; Shigeno, Asako ; Fujikura, Daisuke ; Hang'ombe, Bernard M ; Mweene, Aaron S ; Mutemwa, Alisheke ; Squarre, David ; Yamada, Masao ; Higashi, Hideaki ; Sawa, Hirofumi ; Takada, Ayato. / Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum). In: Viruses. 2017 ; Vol. 9, No. 12.
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