Characterization of a mutant of Vibrio vulnificus for heme utilization

Shin-ichi Miyoshi, Yu Inami, Yuko Moriya, Takehito Kamei, Md Monzur Rahman, Shigeo Yamamoto, Ken ichi Tomochika, Sumio Shinoda

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Vibrio vulnificus, an opportunistic human pathogen, can obtain iron from a variety of heme proteins. This process involves the digestion of heme proteins by an exoprotease to liberate protoheme (iron-protoporphyrin IX). In the present study, we isolated and characterized a mutant for protoheme utilization. One mutant isolated by treatment with a chemical mutagen was shown to be unable to use either protoheme or heme proteins, but multiplied in a medium supplemented with an iron siderophore, such as iron-vulnibactin. Like a wild-type strain, the mutant sensed iron depletion, so that the 74- and 79-kDa outer membrane proteins were expressed under iron-regulated conditions. Both the parent and mutant strains secreted hemolysin independent of the iron concentration of the medium. Whole cells of either of the strains were equally capable of binding of hematin. Taken together, the data suggest that the mutant may have a mutation in a gene encoding an inner membrane or a periplasmic protein which transports protoheme or iron dissociated from protoporphyrin IX into the cell.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalFEMS Microbiology Letters
Volume148
Issue number1
DOIs
Publication statusPublished - Mar 1 1997

Fingerprint

Vibrio vulnificus
Heme
Iron
Hemeproteins
Exopeptidases
Periplasmic Proteins
Siderophores
Hemin
Hemolysin Proteins
Mutagens
Protein Transport
Digestion
Membrane Proteins
Mutation
Membranes
Genes

Keywords

  • heme utilization
  • pathogenesis
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Miyoshi, S., Inami, Y., Moriya, Y., Kamei, T., Rahman, M. M., Yamamoto, S., ... Shinoda, S. (1997). Characterization of a mutant of Vibrio vulnificus for heme utilization. FEMS Microbiology Letters, 148(1), 101-106. https://doi.org/10.1016/S0378-1097(97)00022-0

Characterization of a mutant of Vibrio vulnificus for heme utilization. / Miyoshi, Shin-ichi; Inami, Yu; Moriya, Yuko; Kamei, Takehito; Rahman, Md Monzur; Yamamoto, Shigeo; Tomochika, Ken ichi; Shinoda, Sumio.

In: FEMS Microbiology Letters, Vol. 148, No. 1, 01.03.1997, p. 101-106.

Research output: Contribution to journalArticle

Miyoshi, S, Inami, Y, Moriya, Y, Kamei, T, Rahman, MM, Yamamoto, S, Tomochika, KI & Shinoda, S 1997, 'Characterization of a mutant of Vibrio vulnificus for heme utilization', FEMS Microbiology Letters, vol. 148, no. 1, pp. 101-106. https://doi.org/10.1016/S0378-1097(97)00022-0
Miyoshi, Shin-ichi ; Inami, Yu ; Moriya, Yuko ; Kamei, Takehito ; Rahman, Md Monzur ; Yamamoto, Shigeo ; Tomochika, Ken ichi ; Shinoda, Sumio. / Characterization of a mutant of Vibrio vulnificus for heme utilization. In: FEMS Microbiology Letters. 1997 ; Vol. 148, No. 1. pp. 101-106.
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