Characterization of a genomic region that maintains chlorophyll and nitrogen contents during ripening in a high-yielding stay-green rice cultivar

Toshio Yamamoto, Tadafumi Suzuki, Kenji Suzuki, Shunsuke Adachi, Jian Sun, Masahiro Yano, Taiichiro Ookawa, Tadashi Hirasawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The high-yielding indica × japonica rice (Oryza sativa L.) cultivar Akenohoshi tends to maintain a higher photosynthetic rate during ripening owing to its higher nitrogen accumulation and nitrogen partitioning to leaves than the commercial japonica cultivar Koshihikari. By using recombinant inbred lines derived from a cross between Akenohoshi and Koshihikari, we detected at least 6 quantitative trait loci (QTLs) for maintaining higher leaf chlorophyll content, 4 QTLs for nitrogen content, and 5 QTLs for the rate of nitrogen transport to shoots during ripening in the paddy field. Then we developed two chromosome segment substitution lines carrying Akenohoshi segments on the short arm of chromosome 3, where the QTLs for chlorophyll content reduction were clustered, in the Koshihikari genetic background. The lines showed higher rate of nitrogen transport to shoots, leaf chlorophyll and nitrogen contents, and therefore a higher rate of leaf photosynthesis, than Koshihikari. We concluded that a 7.7-Mb region present in both two lines, named qCHR1, is important for maintaining chlorophyll and nitrogen contents during senescence. The Akenohoshi allele at qCHR1 increased nitrogen accumulation and nitrogen partitioning to leaves during ripening, but did not change yield.

Original languageEnglish
Pages (from-to)54-64
Number of pages11
JournalField Crops Research
Volume206
DOIs
Publication statusPublished - May 1 2017
Externally publishedYes

Fingerprint

ripening
nitrogen content
cultivar
genomics
chlorophyll
rice
quantitative trait loci
nitrogen
cultivars
leaves
chromosomes
substitution lines
shoots
chromosome
partitioning
shoot
genetic background
inbred lines
paddies
Oryza sativa

Keywords

  • Leaf senescence
  • Nitrogen content
  • Photosynthesis
  • Quantitative trait locus
  • Rice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science

Cite this

Characterization of a genomic region that maintains chlorophyll and nitrogen contents during ripening in a high-yielding stay-green rice cultivar. / Yamamoto, Toshio; Suzuki, Tadafumi; Suzuki, Kenji; Adachi, Shunsuke; Sun, Jian; Yano, Masahiro; Ookawa, Taiichiro; Hirasawa, Tadashi.

In: Field Crops Research, Vol. 206, 01.05.2017, p. 54-64.

Research output: Contribution to journalArticle

Yamamoto, Toshio ; Suzuki, Tadafumi ; Suzuki, Kenji ; Adachi, Shunsuke ; Sun, Jian ; Yano, Masahiro ; Ookawa, Taiichiro ; Hirasawa, Tadashi. / Characterization of a genomic region that maintains chlorophyll and nitrogen contents during ripening in a high-yielding stay-green rice cultivar. In: Field Crops Research. 2017 ; Vol. 206. pp. 54-64.
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