TY - JOUR
T1 - Characterization of a Cl--channel from rabbit transverse tubules in the planar lipid bilayer system
AU - Hidaka, Jun
AU - Ide, Toru
AU - Kawasaki, Takashi
AU - Taguchi, Takahisa
AU - Kasai, Michiki
PY - 1993/3/31
Y1 - 1993/3/31
N2 - A Cl--channel of the transverse tubule(TT) was incorporated into artificial planar lipid bilayers and its properties were investigated. The channel was found to have the following properties. (1) The single-channel conductance is 40 pS in choline-Cl solution. (2) The rate of gating of the channel does not depend on the membrane voltage. (3) The channel is blocked by stilbene derivatives (DIDS and SITS), which are known as inhibitors of voltage-dependent Cl--channels of the Torpedo electric organ, from both sides of the membrane. (4) An inhibitor of voltage-dependent Cl--channels of skeletal muscle, 9-anthracene carboxylic acid(9-AC), inhibits Cl--current from the cis-side to which the TT vesicles were added.
AB - A Cl--channel of the transverse tubule(TT) was incorporated into artificial planar lipid bilayers and its properties were investigated. The channel was found to have the following properties. (1) The single-channel conductance is 40 pS in choline-Cl solution. (2) The rate of gating of the channel does not depend on the membrane voltage. (3) The channel is blocked by stilbene derivatives (DIDS and SITS), which are known as inhibitors of voltage-dependent Cl--channels of the Torpedo electric organ, from both sides of the membrane. (4) An inhibitor of voltage-dependent Cl--channels of skeletal muscle, 9-anthracene carboxylic acid(9-AC), inhibits Cl--current from the cis-side to which the TT vesicles were added.
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U2 - 10.1006/bbrc.1993.1313
DO - 10.1006/bbrc.1993.1313
M3 - Article
C2 - 8385458
AN - SCOPUS:0027261548
VL - 191
SP - 977
EP - 982
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -