Characterization of a Cl--channel from rabbit transverse tubules in the planar lipid bilayer system

Jun Hidaka; Toru Ide; Takashi Kawasaki; Takahisa Taguchi; Michiki Kasai

doi:10.1006/bbrc.1993.1313

Characterization of a Cl^--channel from rabbit transverse tubules in the planar lipid bilayer system

Jun Hidaka, Toru Ide, Takashi Kawasaki, Takahisa Taguchi, Michiki Kasai

Research output: Contribution to journal › Article

6 Citations (Scopus)

Abstract

A Cl^--channel of the transverse tubule(TT) was incorporated into artificial planar lipid bilayers and its properties were investigated. The channel was found to have the following properties. (1) The single-channel conductance is 40 pS in choline-Cl solution. (2) The rate of gating of the channel does not depend on the membrane voltage. (3) The channel is blocked by stilbene derivatives (DIDS and SITS), which are known as inhibitors of voltage-dependent Cl^--channels of the Torpedo electric organ, from both sides of the membrane. (4) An inhibitor of voltage-dependent Cl^--channels of skeletal muscle, 9-anthracene carboxylic acid(9-AC), inhibits Cl^--current from the cis-side to which the TT vesicles were added.

Original language	English
Pages (from-to)	977-982
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	191
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1993.1313
Publication status	Published - Mar 31 1993
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1006/bbrc.1993.1313

Fingerprint Dive into the research topics of 'Characterization of a Cl<sup>-</sup>-channel from rabbit transverse tubules in the planar lipid bilayer system'. Together they form a unique fingerprint.

Cite this

Hidaka, J., Ide, T., Kawasaki, T., Taguchi, T., & Kasai, M. (1993). Characterization of a Cl^--channel from rabbit transverse tubules in the planar lipid bilayer system. Biochemical and Biophysical Research Communications, 191(3), 977-982. https://doi.org/10.1006/bbrc.1993.1313

@article{d303fd8f7b7947c8816cfa0f98f3fb8e,

title = "Characterization of a Cl--channel from rabbit transverse tubules in the planar lipid bilayer system",

abstract = "A Cl--channel of the transverse tubule(TT) was incorporated into artificial planar lipid bilayers and its properties were investigated. The channel was found to have the following properties. (1) The single-channel conductance is 40 pS in choline-Cl solution. (2) The rate of gating of the channel does not depend on the membrane voltage. (3) The channel is blocked by stilbene derivatives (DIDS and SITS), which are known as inhibitors of voltage-dependent Cl--channels of the Torpedo electric organ, from both sides of the membrane. (4) An inhibitor of voltage-dependent Cl--channels of skeletal muscle, 9-anthracene carboxylic acid(9-AC), inhibits Cl--current from the cis-side to which the TT vesicles were added.",

author = "Jun Hidaka and Toru Ide and Takashi Kawasaki and Takahisa Taguchi and Michiki Kasai",

year = "1993",

month = mar,

day = "31",

doi = "10.1006/bbrc.1993.1313",

language = "English",

volume = "191",

pages = "977--982",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Characterization of a Cl--channel from rabbit transverse tubules in the planar lipid bilayer system

AU - Hidaka, Jun

AU - Ide, Toru

AU - Kawasaki, Takashi

AU - Taguchi, Takahisa

AU - Kasai, Michiki

PY - 1993/3/31

Y1 - 1993/3/31

N2 - A Cl--channel of the transverse tubule(TT) was incorporated into artificial planar lipid bilayers and its properties were investigated. The channel was found to have the following properties. (1) The single-channel conductance is 40 pS in choline-Cl solution. (2) The rate of gating of the channel does not depend on the membrane voltage. (3) The channel is blocked by stilbene derivatives (DIDS and SITS), which are known as inhibitors of voltage-dependent Cl--channels of the Torpedo electric organ, from both sides of the membrane. (4) An inhibitor of voltage-dependent Cl--channels of skeletal muscle, 9-anthracene carboxylic acid(9-AC), inhibits Cl--current from the cis-side to which the TT vesicles were added.

AB - A Cl--channel of the transverse tubule(TT) was incorporated into artificial planar lipid bilayers and its properties were investigated. The channel was found to have the following properties. (1) The single-channel conductance is 40 pS in choline-Cl solution. (2) The rate of gating of the channel does not depend on the membrane voltage. (3) The channel is blocked by stilbene derivatives (DIDS and SITS), which are known as inhibitors of voltage-dependent Cl--channels of the Torpedo electric organ, from both sides of the membrane. (4) An inhibitor of voltage-dependent Cl--channels of skeletal muscle, 9-anthracene carboxylic acid(9-AC), inhibits Cl--current from the cis-side to which the TT vesicles were added.

UR - http://www.scopus.com/inward/record.url?scp=0027261548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027261548&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1993.1313

DO - 10.1006/bbrc.1993.1313

M3 - Article

C2 - 8385458

AN - SCOPUS:0027261548

VL - 191

SP - 977

EP - 982

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -