Characterization of a Cl--Channel from Rabbit Transverse Tubules in the Planar Lipid Bilayer System

J. Hidaka, Toru Ide, T. Kawasaki, T. Taguchi, M. Kasai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A Cl--channel of the transverse tubule(TT) was incorporated into artificial planar lipid bilayers and its properties were investigated. The channel was found to have the following properties. (1) The single-channel conductance is 40 pS in choline-Cl solution. (2) The rate of gating of the channel does not depend on the membrane voltage. (3) The channel is blocked by stilbene derivatives (DIDS and SITS), which are known as inhibitors of voltage-dependent Cl--channels of the Torpedo electric organ, from both sides of the membrane. (4) An inhibitor of voltage-dependent Cl--channels of skeletal muscle, 9-anthracene carboxylic acid(9-AC), inhibits Cl--current from the cis-side to which the TT vesicles were added.

Original languageEnglish
Pages (from-to)977-982
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume191
Issue number3
DOIs
Publication statusPublished - Mar 31 1993
Externally publishedYes

Fingerprint

Lipid bilayers
Lipid Bilayers
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
Electric Organ
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Rabbits
Torpedo
Stilbenes
Membranes
Electric potential
Choline
Skeletal Muscle
Muscle
Derivatives
9-anthroic acid

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Characterization of a Cl--Channel from Rabbit Transverse Tubules in the Planar Lipid Bilayer System. / Hidaka, J.; Ide, Toru; Kawasaki, T.; Taguchi, T.; Kasai, M.

In: Biochemical and Biophysical Research Communications, Vol. 191, No. 3, 31.03.1993, p. 977-982.

Research output: Contribution to journalArticle

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