Characterization of a CENP-C homolog in Arabidopsis thaliana

Yutaka Ogura, Fukashi Shibata, Hiroshi Sato, Minoru Murata

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Centromere protein C (CENP-C) is a component of the kinetochore essential for correct segregation of sister chromatids in mammals. In Arabidopsis thaliana, a single-copy gene encoding a protein homologous to CENP-C has been found by homology in the whole-genome sequence. To investigate the CENP-C homolog (AtCENP-C), we cloned cDNAs by RT-PCR and determined its full-length coding sequence. Antibodies against the synthetic peptide for the C-terminal residues of AtCENP-C detected a polypeptide in Arabidopsis cell extracts on western blots. Immunofluorescence labeling with the antibodies and fluorescence in situ hybridization demonstrated clearly that AtCENP-C is present at the centromeric regions throughout the cell cycle.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalGenes and Genetic Systems
Volume79
Issue number3
DOIs
Publication statusPublished - Jun 2004

Fingerprint

centromeres
Arabidopsis
Arabidopsis thaliana
Kinetochores
Chromatids
Gene encoding
Mammals
proteins
Antibodies
Cell Extracts
Fluorescence In Situ Hybridization
kinetochores
Labeling
antibodies
Fluorescent Antibody Technique
chromatids
synthetic peptides
Cell Cycle
Complementary DNA
Genes

Keywords

  • 180-bp repeat
  • Arabidopsis thaliana
  • CENP-C
  • Centromere
  • Immunofluorescence

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Genetics
  • Genetics(clinical)

Cite this

Characterization of a CENP-C homolog in Arabidopsis thaliana. / Ogura, Yutaka; Shibata, Fukashi; Sato, Hiroshi; Murata, Minoru.

In: Genes and Genetic Systems, Vol. 79, No. 3, 06.2004, p. 139-144.

Research output: Contribution to journalArticle

Ogura, Y, Shibata, F, Sato, H & Murata, M 2004, 'Characterization of a CENP-C homolog in Arabidopsis thaliana', Genes and Genetic Systems, vol. 79, no. 3, pp. 139-144. https://doi.org/10.1266/ggs.79.139
Ogura, Yutaka ; Shibata, Fukashi ; Sato, Hiroshi ; Murata, Minoru. / Characterization of a CENP-C homolog in Arabidopsis thaliana. In: Genes and Genetic Systems. 2004 ; Vol. 79, No. 3. pp. 139-144.
@article{8446b9a67bc04bc1ac80efcdd6f6b13b,
title = "Characterization of a CENP-C homolog in Arabidopsis thaliana",
abstract = "Centromere protein C (CENP-C) is a component of the kinetochore essential for correct segregation of sister chromatids in mammals. In Arabidopsis thaliana, a single-copy gene encoding a protein homologous to CENP-C has been found by homology in the whole-genome sequence. To investigate the CENP-C homolog (AtCENP-C), we cloned cDNAs by RT-PCR and determined its full-length coding sequence. Antibodies against the synthetic peptide for the C-terminal residues of AtCENP-C detected a polypeptide in Arabidopsis cell extracts on western blots. Immunofluorescence labeling with the antibodies and fluorescence in situ hybridization demonstrated clearly that AtCENP-C is present at the centromeric regions throughout the cell cycle.",
keywords = "180-bp repeat, Arabidopsis thaliana, CENP-C, Centromere, Immunofluorescence",
author = "Yutaka Ogura and Fukashi Shibata and Hiroshi Sato and Minoru Murata",
year = "2004",
month = "6",
doi = "10.1266/ggs.79.139",
language = "English",
volume = "79",
pages = "139--144",
journal = "Genes and Genetic Systems",
issn = "1341-7568",
publisher = "Genetics Society of Japan",
number = "3",

}

TY - JOUR

T1 - Characterization of a CENP-C homolog in Arabidopsis thaliana

AU - Ogura, Yutaka

AU - Shibata, Fukashi

AU - Sato, Hiroshi

AU - Murata, Minoru

PY - 2004/6

Y1 - 2004/6

N2 - Centromere protein C (CENP-C) is a component of the kinetochore essential for correct segregation of sister chromatids in mammals. In Arabidopsis thaliana, a single-copy gene encoding a protein homologous to CENP-C has been found by homology in the whole-genome sequence. To investigate the CENP-C homolog (AtCENP-C), we cloned cDNAs by RT-PCR and determined its full-length coding sequence. Antibodies against the synthetic peptide for the C-terminal residues of AtCENP-C detected a polypeptide in Arabidopsis cell extracts on western blots. Immunofluorescence labeling with the antibodies and fluorescence in situ hybridization demonstrated clearly that AtCENP-C is present at the centromeric regions throughout the cell cycle.

AB - Centromere protein C (CENP-C) is a component of the kinetochore essential for correct segregation of sister chromatids in mammals. In Arabidopsis thaliana, a single-copy gene encoding a protein homologous to CENP-C has been found by homology in the whole-genome sequence. To investigate the CENP-C homolog (AtCENP-C), we cloned cDNAs by RT-PCR and determined its full-length coding sequence. Antibodies against the synthetic peptide for the C-terminal residues of AtCENP-C detected a polypeptide in Arabidopsis cell extracts on western blots. Immunofluorescence labeling with the antibodies and fluorescence in situ hybridization demonstrated clearly that AtCENP-C is present at the centromeric regions throughout the cell cycle.

KW - 180-bp repeat

KW - Arabidopsis thaliana

KW - CENP-C

KW - Centromere

KW - Immunofluorescence

UR - http://www.scopus.com/inward/record.url?scp=4344606370&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4344606370&partnerID=8YFLogxK

U2 - 10.1266/ggs.79.139

DO - 10.1266/ggs.79.139

M3 - Article

VL - 79

SP - 139

EP - 144

JO - Genes and Genetic Systems

JF - Genes and Genetic Systems

SN - 1341-7568

IS - 3

ER -