Characterization of a CENP-C homolog in Arabidopsis thaliana

Yutaka Ogura, Fukashi Shibata, Hiroshi Sato, Minoru Murata

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Centromere protein C (CENP-C) is a component of the kinetochore essential for correct segregation of sister chromatids in mammals. In Arabidopsis thaliana, a single-copy gene encoding a protein homologous to CENP-C has been found by homology in the whole-genome sequence. To investigate the CENP-C homolog (AtCENP-C), we cloned cDNAs by RT-PCR and determined its full-length coding sequence. Antibodies against the synthetic peptide for the C-terminal residues of AtCENP-C detected a polypeptide in Arabidopsis cell extracts on western blots. Immunofluorescence labeling with the antibodies and fluorescence in situ hybridization demonstrated clearly that AtCENP-C is present at the centromeric regions throughout the cell cycle.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalGenes and Genetic Systems
Volume79
Issue number3
DOIs
Publication statusPublished - Jun 1 2004

Keywords

  • 180-bp repeat
  • Arabidopsis thaliana
  • CENP-C
  • Centromere
  • Immunofluorescence

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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