Characterization and radio-resistant function of manganese superoxide dismutase of rubrobacter radiotolerans

Hiroaki Terato, Katsuyuki Suzuki, Nobuhiro Nishioka, Atsushi Okamoto, Yuka Shimazaki-Tokuyama, Yuko Inoue, Takeshi Saito

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Rubrobacter radiotolerans is the most radio-resistant eubacterium without spore-formation in the life cycle, and its D37 is 16,000 Gy against gamma-rays. To understand the molecular mechanism of the high radio-resistance, we purified and characterized superoxide dismutase (SOD) of this organism as enzymatic radical scavenger, and then analyzed its genetic information. The purified SOD protein formed homo-tetramerization of 24,000 Da-monomer, while maintaining its enzymatic activity against potassium cyanide and hydrogen peroxide. We obtained a partial amino acid sequence of the protein and cloned the gene from it. Sequence analysis of the cloned gene indicated that the protein showed a similarity to other bacterial manganese SODs (Mn-SODs). Sequencing for adjacent regions of the gene showed that the gene had promoter elements with an open reading frame for putative PAS/PAC sensor protein at the 5'-adjacent region. Introduction of the gene into Escherichia coli cells lacking intrinsic SOD genes restored the cellular enzymatic activity and resistance to methyl viologen, indicating the gene at work. A mutant cell harboring this gene also became resistant against gamma-rays. The present results suggest that the protein in question is the Mn-SOD of R. radiotolerans, a good candidate as a radio-protection factor for this bacterial radio-resistance.

Original languageEnglish
Pages (from-to)735-742
Number of pages8
JournalJournal of Radiation Research
Volume52
Issue number6
DOIs
Publication statusPublished - Nov 1 2011
Externally publishedYes

Fingerprint

inorganic peroxides
Radio
genes
Superoxide Dismutase
manganese
Genes
proteins
Gamma Rays
Proteins
Potassium Cyanide
Eubacterium
Paraquat
gamma rays
PAS
Manganese
Life Cycle Stages
Spores
Hydrogen Peroxide
spores
Open Reading Frames

Keywords

  • Radical scavenger
  • Radio-resistance
  • Rubrobacter radiotolerans
  • Superoxide dismutase

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Health, Toxicology and Mutagenesis

Cite this

Characterization and radio-resistant function of manganese superoxide dismutase of rubrobacter radiotolerans. / Terato, Hiroaki; Suzuki, Katsuyuki; Nishioka, Nobuhiro; Okamoto, Atsushi; Shimazaki-Tokuyama, Yuka; Inoue, Yuko; Saito, Takeshi.

In: Journal of Radiation Research, Vol. 52, No. 6, 01.11.2011, p. 735-742.

Research output: Contribution to journalArticle

Terato, H, Suzuki, K, Nishioka, N, Okamoto, A, Shimazaki-Tokuyama, Y, Inoue, Y & Saito, T 2011, 'Characterization and radio-resistant function of manganese superoxide dismutase of rubrobacter radiotolerans', Journal of Radiation Research, vol. 52, no. 6, pp. 735-742. https://doi.org/10.1269/jrr.11105
Terato, Hiroaki ; Suzuki, Katsuyuki ; Nishioka, Nobuhiro ; Okamoto, Atsushi ; Shimazaki-Tokuyama, Yuka ; Inoue, Yuko ; Saito, Takeshi. / Characterization and radio-resistant function of manganese superoxide dismutase of rubrobacter radiotolerans. In: Journal of Radiation Research. 2011 ; Vol. 52, No. 6. pp. 735-742.
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