Characteristics of serine acetyltransferase from escherichia coli deleting different lengths of amino acid residues from the C-terminus

Koshiki Mino, Kenji Hiraoka, Koreyoshi Imamura, Takaharu Sakiyama, Naoki Eisaki, Asahi Matsuyama, Kazuhiro Nakanishi

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Some properties of serine acetyltransferases (SATs) from Escherichia coli, deleting 10-25 amino acid residues from the C-terminus (SATΔC10-ΔC25) were investigated. The specific activity depended only slightly on the length of the C-terminal region deleted. Although the sensitivity of SATΔC10 to inhibition by L-cysteine was similar to that for the wild-type SAT, it became less with further increases in the length of the amino acid residues deleted. SATΔC10 was inactivated on cooling to 0°C and dissociated into dimers or trimers in the same manner as the wild-type SAT, but Met-256-Ile mutant SAT as well as SATΔC14, SATΔC20, and SATΔC25 were stable. Since SATΔC10, SATΔC14, and SATΔC25 did not form a complex with O-acetylserine sulfhydrylase-A (OASS-A) in a way similar to SATΔC20, it was indicated that 10 amino acid residues or fewer from the C-terminus of the wild-type SAT are responsible for the complex formation with OASS-A. The C-terminal peptide of the 10 amino acid residues interacted competitively with OASS-A with respect to OAS although its affinity was much lower than that for the wild-type SAT.

Original languageEnglish
Pages (from-to)1874-1880
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume64
Issue number9
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • C-terminal region
  • Cold inactivation
  • Cysteine synthetase
  • O-acetylserine sulfhydrylase
  • Serine acetyltransferase

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Characteristics of serine acetyltransferase from escherichia coli deleting different lengths of amino acid residues from the C-terminus'. Together they form a unique fingerprint.

  • Cite this