Characteristics of compacted plasmid DNA by high pressurization.

Tsuyoshi Kimura, Kana Horiuchi, Kimio Kurita, Tsutomu Ono, Hidekazu Yoshizawa, Toshiya Fujisato, Akio Kishida

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In order to investigate the effect of pressure on the tertiary structure of plasmid DNA having the supercoiled and relaxed forms, the solution of plasmid DNA was hydrostatically pressurized at different atmosphere and 40 degrees C for various times. For dynamic light scattering (DLS) measurement of the pressurized plasmid DNA, the hydrodynamic diameters of the super-coiled and relaxed plasmid DNA were decreased with increasing pressure. Also, at constant pressure, a long period of pressure treatment effectively induced the decrease in plasmid DNA. These results suggest that the plasmid DNA was condensed by high hydrostatic pressurization. The circular dichroism (CD) spectrum of the pressurized plasmid DNA was slightly changed. For digestion by S1 nuclease, which selectively cleaves single strand DNA, the pressurized plasmid DNA was easily degraded compared to the non-pressurized plasmid DNA, suggesting that the double helix of plasmid DNA was partly dissociated to single strand by the pressure-induced compaction of plasmid DNA. These results indicate that high hydrostatic pressurization is one of powerful tools for preparing the compacted plasmid DNA.

Original languageEnglish
Pages (from-to)343-344
Number of pages2
JournalNucleic acids symposium series (2004)
Volume51
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

Plasmids
DNA
Pressure
Superhelical DNA
Hydrodynamics
Circular Dichroism
Atmosphere
Digestion

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Kimura, T., Horiuchi, K., Kurita, K., Ono, T., Yoshizawa, H., Fujisato, T., & Kishida, A. (2007). Characteristics of compacted plasmid DNA by high pressurization. Nucleic acids symposium series (2004), 51, 343-344.

Characteristics of compacted plasmid DNA by high pressurization. / Kimura, Tsuyoshi; Horiuchi, Kana; Kurita, Kimio; Ono, Tsutomu; Yoshizawa, Hidekazu; Fujisato, Toshiya; Kishida, Akio.

In: Nucleic acids symposium series (2004), Vol. 51, 2007, p. 343-344.

Research output: Contribution to journalArticle

Kimura, T, Horiuchi, K, Kurita, K, Ono, T, Yoshizawa, H, Fujisato, T & Kishida, A 2007, 'Characteristics of compacted plasmid DNA by high pressurization.', Nucleic acids symposium series (2004), vol. 51, pp. 343-344.
Kimura T, Horiuchi K, Kurita K, Ono T, Yoshizawa H, Fujisato T et al. Characteristics of compacted plasmid DNA by high pressurization. Nucleic acids symposium series (2004). 2007;51:343-344.
Kimura, Tsuyoshi ; Horiuchi, Kana ; Kurita, Kimio ; Ono, Tsutomu ; Yoshizawa, Hidekazu ; Fujisato, Toshiya ; Kishida, Akio. / Characteristics of compacted plasmid DNA by high pressurization. In: Nucleic acids symposium series (2004). 2007 ; Vol. 51. pp. 343-344.
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