Characteristics of compacted plasmid DNA by high pressurization.

Tsuyoshi Kimura, Kana Horiuchi, Kimio Kurita, Tsutomu Ono, Hidekazu Yoshizawa, Toshiya Fujisato, Akio Kishida

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In order to investigate the effect of pressure on the tertiary structure of plasmid DNA having the supercoiled and relaxed forms, the solution of plasmid DNA was hydrostatically pressurized at different atmosphere and 40 degrees C for various times. For dynamic light scattering (DLS) measurement of the pressurized plasmid DNA, the hydrodynamic diameters of the super-coiled and relaxed plasmid DNA were decreased with increasing pressure. Also, at constant pressure, a long period of pressure treatment effectively induced the decrease in plasmid DNA. These results suggest that the plasmid DNA was condensed by high hydrostatic pressurization. The circular dichroism (CD) spectrum of the pressurized plasmid DNA was slightly changed. For digestion by S1 nuclease, which selectively cleaves single strand DNA, the pressurized plasmid DNA was easily degraded compared to the non-pressurized plasmid DNA, suggesting that the double helix of plasmid DNA was partly dissociated to single strand by the pressure-induced compaction of plasmid DNA. These results indicate that high hydrostatic pressurization is one of powerful tools for preparing the compacted plasmid DNA.

Original languageEnglish
Pages (from-to)343-344
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
DOIs
Publication statusPublished - 2007

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Characteristics of compacted plasmid DNA by high pressurization.'. Together they form a unique fingerprint.

  • Cite this

    Kimura, T., Horiuchi, K., Kurita, K., Ono, T., Yoshizawa, H., Fujisato, T., & Kishida, A. (2007). Characteristics of compacted plasmid DNA by high pressurization. Nucleic acids symposium series (2004), (51), 343-344. https://doi.org/10.1093/nass/nrm172