Characteristics of CD133+ human colon cancer SW620 cells

Hironobu Kawamoto, Takeshi Yuasa, Yasuhiro Kubota, Masayuki Seita, Hiromi Sasamoto, Javed M. Shahid, Takahiro Hayashi, Hiroyuki Nakahara, Reham Hassan, Masaya Iwamuro, Eisaku Kondo, Shuhei Nakaji, Noriaki Tanaka, Naoya Kobayashi

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Worldwide, colorectal cancer is the third most common type of cancer affecting both sexes. It has been proposed that a small subset of cancer cells (cancer stem cells) within each tumor is able to initiate tumor growth. In 2007, two research groups simultaneously identified a colon cancer stem cell population in human tumors by the use of CD133 expression. In the present study, we used a human colon cancer cell line, SW620, to analyze the cancer stem cell-like characteristics of CD133+ cells in vitro and in vivo. In vitro, CD133+ SW620 cells had a higher proliferative capacity, were more irradiation- and chemotherapy-resistant, and had a higher expression of β-catenin compared with CD133- cells. Injections of either CD133+ or CD133- cells into the skin or rectal mucosa of NOD/SCID mice led to tumors; however, injection of CD133+ cells resulted in the formation of larger tumors. Tumors derived from injections of CD133- cells did not contain any CD133+ cells, whereas tumors derived from injections of CD133+ cells did contain CD133 + cells, suggesting self-renewing capability. However, the proportion of CD133+ cells in the newly formed tumors in vivo was lower than the proportion of CD133+ cells in vitro. In conclusion, the human colon cancer cell line, SW620, contains both CD133+ and CD133 - phenotypes, and the CD133+ phenotype has characteristics consistent with those of cancer stem cells.

Original languageEnglish
Pages (from-to)857-864
Number of pages8
JournalCell Transplantation
Volume19
Issue number6-7
DOIs
Publication statusPublished - 2010

Fingerprint

Colonic Neoplasms
Tumors
Stem cells
Neoplastic Stem Cells
Neoplasms
Cells
Injections
Chemotherapy
Phenotype
Cell Line
Catenins
Skin
Inbred NOD Mouse
SCID Mice
Irradiation
Colorectal Neoplasms
Mucous Membrane
Drug Therapy

Keywords

  • CD133
  • Colon cancer
  • Tumorigenesis

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

Cite this

Kawamoto, H., Yuasa, T., Kubota, Y., Seita, M., Sasamoto, H., Shahid, J. M., ... Kobayashi, N. (2010). Characteristics of CD133+ human colon cancer SW620 cells. Cell Transplantation, 19(6-7), 857-864. https://doi.org/10.3727/096368910X508988

Characteristics of CD133+ human colon cancer SW620 cells. / Kawamoto, Hironobu; Yuasa, Takeshi; Kubota, Yasuhiro; Seita, Masayuki; Sasamoto, Hiromi; Shahid, Javed M.; Hayashi, Takahiro; Nakahara, Hiroyuki; Hassan, Reham; Iwamuro, Masaya; Kondo, Eisaku; Nakaji, Shuhei; Tanaka, Noriaki; Kobayashi, Naoya.

In: Cell Transplantation, Vol. 19, No. 6-7, 2010, p. 857-864.

Research output: Contribution to journalArticle

Kawamoto, H, Yuasa, T, Kubota, Y, Seita, M, Sasamoto, H, Shahid, JM, Hayashi, T, Nakahara, H, Hassan, R, Iwamuro, M, Kondo, E, Nakaji, S, Tanaka, N & Kobayashi, N 2010, 'Characteristics of CD133+ human colon cancer SW620 cells', Cell Transplantation, vol. 19, no. 6-7, pp. 857-864. https://doi.org/10.3727/096368910X508988
Kawamoto H, Yuasa T, Kubota Y, Seita M, Sasamoto H, Shahid JM et al. Characteristics of CD133+ human colon cancer SW620 cells. Cell Transplantation. 2010;19(6-7):857-864. https://doi.org/10.3727/096368910X508988
Kawamoto, Hironobu ; Yuasa, Takeshi ; Kubota, Yasuhiro ; Seita, Masayuki ; Sasamoto, Hiromi ; Shahid, Javed M. ; Hayashi, Takahiro ; Nakahara, Hiroyuki ; Hassan, Reham ; Iwamuro, Masaya ; Kondo, Eisaku ; Nakaji, Shuhei ; Tanaka, Noriaki ; Kobayashi, Naoya. / Characteristics of CD133+ human colon cancer SW620 cells. In: Cell Transplantation. 2010 ; Vol. 19, No. 6-7. pp. 857-864.
@article{ba073bd7fc30441f888ee1707bcd8f2f,
title = "Characteristics of CD133+ human colon cancer SW620 cells",
abstract = "Worldwide, colorectal cancer is the third most common type of cancer affecting both sexes. It has been proposed that a small subset of cancer cells (cancer stem cells) within each tumor is able to initiate tumor growth. In 2007, two research groups simultaneously identified a colon cancer stem cell population in human tumors by the use of CD133 expression. In the present study, we used a human colon cancer cell line, SW620, to analyze the cancer stem cell-like characteristics of CD133+ cells in vitro and in vivo. In vitro, CD133+ SW620 cells had a higher proliferative capacity, were more irradiation- and chemotherapy-resistant, and had a higher expression of β-catenin compared with CD133- cells. Injections of either CD133+ or CD133- cells into the skin or rectal mucosa of NOD/SCID mice led to tumors; however, injection of CD133+ cells resulted in the formation of larger tumors. Tumors derived from injections of CD133- cells did not contain any CD133+ cells, whereas tumors derived from injections of CD133+ cells did contain CD133 + cells, suggesting self-renewing capability. However, the proportion of CD133+ cells in the newly formed tumors in vivo was lower than the proportion of CD133+ cells in vitro. In conclusion, the human colon cancer cell line, SW620, contains both CD133+ and CD133 - phenotypes, and the CD133+ phenotype has characteristics consistent with those of cancer stem cells.",
keywords = "CD133, Colon cancer, Tumorigenesis",
author = "Hironobu Kawamoto and Takeshi Yuasa and Yasuhiro Kubota and Masayuki Seita and Hiromi Sasamoto and Shahid, {Javed M.} and Takahiro Hayashi and Hiroyuki Nakahara and Reham Hassan and Masaya Iwamuro and Eisaku Kondo and Shuhei Nakaji and Noriaki Tanaka and Naoya Kobayashi",
year = "2010",
doi = "10.3727/096368910X508988",
language = "English",
volume = "19",
pages = "857--864",
journal = "Cell Transplantation",
issn = "0963-6897",
publisher = "Cognizant Communication Corporation",
number = "6-7",

}

TY - JOUR

T1 - Characteristics of CD133+ human colon cancer SW620 cells

AU - Kawamoto, Hironobu

AU - Yuasa, Takeshi

AU - Kubota, Yasuhiro

AU - Seita, Masayuki

AU - Sasamoto, Hiromi

AU - Shahid, Javed M.

AU - Hayashi, Takahiro

AU - Nakahara, Hiroyuki

AU - Hassan, Reham

AU - Iwamuro, Masaya

AU - Kondo, Eisaku

AU - Nakaji, Shuhei

AU - Tanaka, Noriaki

AU - Kobayashi, Naoya

PY - 2010

Y1 - 2010

N2 - Worldwide, colorectal cancer is the third most common type of cancer affecting both sexes. It has been proposed that a small subset of cancer cells (cancer stem cells) within each tumor is able to initiate tumor growth. In 2007, two research groups simultaneously identified a colon cancer stem cell population in human tumors by the use of CD133 expression. In the present study, we used a human colon cancer cell line, SW620, to analyze the cancer stem cell-like characteristics of CD133+ cells in vitro and in vivo. In vitro, CD133+ SW620 cells had a higher proliferative capacity, were more irradiation- and chemotherapy-resistant, and had a higher expression of β-catenin compared with CD133- cells. Injections of either CD133+ or CD133- cells into the skin or rectal mucosa of NOD/SCID mice led to tumors; however, injection of CD133+ cells resulted in the formation of larger tumors. Tumors derived from injections of CD133- cells did not contain any CD133+ cells, whereas tumors derived from injections of CD133+ cells did contain CD133 + cells, suggesting self-renewing capability. However, the proportion of CD133+ cells in the newly formed tumors in vivo was lower than the proportion of CD133+ cells in vitro. In conclusion, the human colon cancer cell line, SW620, contains both CD133+ and CD133 - phenotypes, and the CD133+ phenotype has characteristics consistent with those of cancer stem cells.

AB - Worldwide, colorectal cancer is the third most common type of cancer affecting both sexes. It has been proposed that a small subset of cancer cells (cancer stem cells) within each tumor is able to initiate tumor growth. In 2007, two research groups simultaneously identified a colon cancer stem cell population in human tumors by the use of CD133 expression. In the present study, we used a human colon cancer cell line, SW620, to analyze the cancer stem cell-like characteristics of CD133+ cells in vitro and in vivo. In vitro, CD133+ SW620 cells had a higher proliferative capacity, were more irradiation- and chemotherapy-resistant, and had a higher expression of β-catenin compared with CD133- cells. Injections of either CD133+ or CD133- cells into the skin or rectal mucosa of NOD/SCID mice led to tumors; however, injection of CD133+ cells resulted in the formation of larger tumors. Tumors derived from injections of CD133- cells did not contain any CD133+ cells, whereas tumors derived from injections of CD133+ cells did contain CD133 + cells, suggesting self-renewing capability. However, the proportion of CD133+ cells in the newly formed tumors in vivo was lower than the proportion of CD133+ cells in vitro. In conclusion, the human colon cancer cell line, SW620, contains both CD133+ and CD133 - phenotypes, and the CD133+ phenotype has characteristics consistent with those of cancer stem cells.

KW - CD133

KW - Colon cancer

KW - Tumorigenesis

UR - http://www.scopus.com/inward/record.url?scp=77958558369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77958558369&partnerID=8YFLogxK

U2 - 10.3727/096368910X508988

DO - 10.3727/096368910X508988

M3 - Article

C2 - 20587144

AN - SCOPUS:77958558369

VL - 19

SP - 857

EP - 864

JO - Cell Transplantation

JF - Cell Transplantation

SN - 0963-6897

IS - 6-7

ER -