Characteristics of biofilm-induced degradation at resin–dentin interfaces using multiple combinations of adhesives and resins

Yuan Zhou, Khairul Matin, Yasushi Shimada, Alireza Sadr, Guoqing Wang, Junji Tagami, Xiping Feng

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: We aimed to evaluate morphological, mechanical and chemical characteristics at resin–dentin interfaces using multiple combinations of adhesives and resins after a short-term biofilm-induced degradation. Methods: Cervical cavities were prepared in bovine incisors, treated by Clearfil SE Bond 2 (SE) or FL-Bond II (FL), restored by Clearfil Majesty ES Flow (ES) or Beautifil Flow Plus (BFP) and grouped into SE-ES, SE-BFP, FL-ES and FL-BFP. After biofilm challenge, interfacial gaps and dentin wall lesions were examined by optical coherence tomography (OCT). Gap depth (GD), gap pattern scale (GPS) and dentin wall lesion depth (WLD) were evaluated from confocal laser scanning microscope. Microhardness of dentin lesions was measured with a Vickers microhardness tester. Chemical elements in resins and dentin wall lesions were analyzed by scanning electron microscopy and energy dispersive X-ray spectrometry (SEM/EDS). Morphological structures of interfacial gaps were observed by SEM. Results: OCT could detect adhesive-dentin-bonded and adhesive-dentin-debonded gaps. SE-containing groups showed significantly lower GPS than FL-containing groups. FL-BFP showed significantly lower WLD than FL-ES. Microhardness of dentin wall lesions was higher than that of outer lesions and they showed significant differences in FL-BFP. SE-BFP showed a lower GPS curve and higher intensities of Ca and P in the upper half of dentin wall lesions than other groups. From SEM, microgaps between filler and matrix, break and loss of matrix, separation of adhesive matrix with hybrid layer occurred at interfacial gaps. Significance: The morphological, mechanical and chemical characteristics of resin–dentin interfacial degradation depend on the component and chemistry of restorative materials.

Original languageEnglish
Pages (from-to)1260-1272
Number of pages13
JournalDental Materials
Volume37
Issue number8
DOIs
Publication statusPublished - Aug 2021

Keywords

  • Adhesive
  • CLSM
  • Cariogenic biofilms
  • Composite resin
  • Dentin wall lesions
  • Interfacial gaps
  • Microhardness
  • Optical coherence tomography (OCT)
  • SEM/EDS

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Fingerprint

Dive into the research topics of 'Characteristics of biofilm-induced degradation at resin–dentin interfaces using multiple combinations of adhesives and resins'. Together they form a unique fingerprint.

Cite this