Characteristics of antibody to denatured DNA in sera of patients with systemic lupus erythematosus and other rheumatic diseases

T. Sarai, S. Miyawaki, N. Kurata, T. Ofuji

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Antibody to native (n-)DNA and denatured (d-)DNA were detected simultaneously and quantitatively in patients who had systemic lupus erythematosus (SLE) and other rheumatic diseases by the Millipore Filter method. In a group of patients who had SLE, 94% had antibody to both n-DNA and d-DNA; 6% had antibody to d-DNA only; serum that had antibody to n-DNA was not found. On the other hand, some of the patients who had progressive systemic sclerosis, dermatomyositis, polymositis, and Sjogren's syndrome had antibody to d-DNA only. In order to estimate the participation of anti-d-DNA antibody to lupus nephritis, patients who had SLE were classified into two groups according to immunofluorescent glomerular stainings. In a group of patients whose sera had lumpy or granular stainings, the sera reacted predominantly with n-DNA. In contrast, in the other group of patients whose sera had mesangial or linear glomerular stainings, the sera had antibodies reactive with d-DNA predominantly. These differences of reactivity between the two groups were statistically significant (P

Original languageEnglish
Pages (from-to)761-766
Number of pages6
JournalAmerican Journal of Clinical Pathology
Volume73
Issue number6
Publication statusPublished - 1980

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Rheumatic Diseases
Systemic Lupus Erythematosus
Antibodies
DNA
Serum
Staining and Labeling
Micropore Filters
Diffuse Scleroderma
Lupus Nephritis
Dermatomyositis
Antinuclear Antibodies
Sjogren's Syndrome

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Characteristics of antibody to denatured DNA in sera of patients with systemic lupus erythematosus and other rheumatic diseases. / Sarai, T.; Miyawaki, S.; Kurata, N.; Ofuji, T.

In: American Journal of Clinical Pathology, Vol. 73, No. 6, 1980, p. 761-766.

Research output: Contribution to journalArticle

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abstract = "Antibody to native (n-)DNA and denatured (d-)DNA were detected simultaneously and quantitatively in patients who had systemic lupus erythematosus (SLE) and other rheumatic diseases by the Millipore Filter method. In a group of patients who had SLE, 94{\%} had antibody to both n-DNA and d-DNA; 6{\%} had antibody to d-DNA only; serum that had antibody to n-DNA was not found. On the other hand, some of the patients who had progressive systemic sclerosis, dermatomyositis, polymositis, and Sjogren's syndrome had antibody to d-DNA only. In order to estimate the participation of anti-d-DNA antibody to lupus nephritis, patients who had SLE were classified into two groups according to immunofluorescent glomerular stainings. In a group of patients whose sera had lumpy or granular stainings, the sera reacted predominantly with n-DNA. In contrast, in the other group of patients whose sera had mesangial or linear glomerular stainings, the sera had antibodies reactive with d-DNA predominantly. These differences of reactivity between the two groups were statistically significant (P",
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N2 - Antibody to native (n-)DNA and denatured (d-)DNA were detected simultaneously and quantitatively in patients who had systemic lupus erythematosus (SLE) and other rheumatic diseases by the Millipore Filter method. In a group of patients who had SLE, 94% had antibody to both n-DNA and d-DNA; 6% had antibody to d-DNA only; serum that had antibody to n-DNA was not found. On the other hand, some of the patients who had progressive systemic sclerosis, dermatomyositis, polymositis, and Sjogren's syndrome had antibody to d-DNA only. In order to estimate the participation of anti-d-DNA antibody to lupus nephritis, patients who had SLE were classified into two groups according to immunofluorescent glomerular stainings. In a group of patients whose sera had lumpy or granular stainings, the sera reacted predominantly with n-DNA. In contrast, in the other group of patients whose sera had mesangial or linear glomerular stainings, the sera had antibodies reactive with d-DNA predominantly. These differences of reactivity between the two groups were statistically significant (P

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