Characteristics of α-glucosidase production from recombinant Aspergillus oryzae by membrane-surface liquid culture in comparison with various cultivation methods

Masakazu Morita, Hiroko Shimamura, Naoyuki Ishida, Koreyoshi Imamura, Takaharu Sakiyama, Kazuhiro Nakanishi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

α-Glucosidase was produced using recombinant Aspergillus oryzae by membrane-surface liquid culture (MSLC), a method previously developed by the authors and the results compared with other methods, including shaking flask culture (SFC), agar-plate culture (APC), culture on urethane sponge supports (USC), and liquid surface culture (LSC) to determine possible reasons for the advantageous features of MSLC. When yeast extract was used as a nitrogen source, the amount of enzyme produced by MSLC was 5 or more times higher than those for SFC and LSC, but similar to that using APC. Enzyme production in USC was slightly lower than in MSLC and APC. Cell growth was similar irrespective of the cultivation method used. When NaNO3, a typical inorganic nitrogen source was used, enzyme production in all the cultures was lower than that using yeast extract. However, even using NaNO3, the amount of the enzyme produced by MSLC was 8 to 20 times higher than those by SFC, APC, USC, and LSC. Although cell growth using NaNO3 was similar to that for yeast extract in MSLC, it was markedly decreased in SFC, APC, and LSC. The reason for the difference in enzyme productivity for various cultivation methods using yeast extract and NaNO3 as a nitrogen source is discussed, on the basis of the experimental findings. The role of the oxygen transfer effect and gene expression levels in enzyme production were also examined.

Original languageEnglish
Pages (from-to)200-206
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume98
Issue number3
Publication statusPublished - Sep 2004

Fingerprint

Glucosidases
Aspergillus oryzae
Aspergillus
Agar
Membranes
Liquids
Enzymes
Yeasts
Nitrogen
Yeast
Cell growth
Urethane
Porifera
Growth
Oxygen
Gene Expression
Gene expression
Productivity

Keywords

  • α-glucosidase
  • Agar-plate culture
  • Aspergillus oryzae
  • Membrane-surface liquid culture
  • Shaking flask culture

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Characteristics of α-glucosidase production from recombinant Aspergillus oryzae by membrane-surface liquid culture in comparison with various cultivation methods. / Morita, Masakazu; Shimamura, Hiroko; Ishida, Naoyuki; Imamura, Koreyoshi; Sakiyama, Takaharu; Nakanishi, Kazuhiro.

In: Journal of Bioscience and Bioengineering, Vol. 98, No. 3, 09.2004, p. 200-206.

Research output: Contribution to journalArticle

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