Changes in urinary enzyme activity and histochemical findings in experimental tubular injury induced by gold sodium thiomalate

Toshio Ogura, Michio Takaoka, Takayoshi Yamauchi, Tetsuya Oishi, Yukari Mimura, Masami Hashimoto, Naoko Asano, Masahiro Yamamura, Fumio Otsuka, Hirofumi Makino, Zensuke Ota, Kayo Takahashi

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

To elucidate the renal injury induced by gold treatment, we administered various doses of gold sodium thiomalate (GST) to Wistar rats and investigated alterations in the urinary enzyme activity, γ-glutamyl transpeptidase (γGTP) and N-acetyl-β-glucosaminidase (NAG) activity, and histochemical change of enzymes, γGTP, alkaline phosphatase (ALP) and acid phosphatase (ACP) activity in the renal tissue. The single administration of a large dose of gold salts induced acute tubular necrosis and enzyme leakage was detected histochemically without damage to the glomerulus. After chronic administration of small doses of gold salts, the urinary γGTP activities gradually increased, but urinary NAG activities did not. These findings suggested that the change in urinary enzyme activities, which leaked from inside of brushborder or lysosome, indicated the degree or localization of tubular damage, because renal tubules were selectively injured by gold salts.

Original languageEnglish
Pages (from-to)41-55
Number of pages15
JournalJournal of Medicine
Volume27
Issue number1-2
Publication statusPublished - Aug 27 1996

Keywords

  • Gold salts
  • acute tubular necrosis
  • histochemistry
  • nephrotoxicity
  • tubulotoxicity
  • urinary enzyme activity

ASJC Scopus subject areas

  • Medicine(all)

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