Changes in the vasculature of bovine corpus luteum during the estrous cycle and prostaglandin F2α-induced luteolysis

Takuo Hojo, Mohamad O. Al-Zi'abi, Dariusz J. Skarzynski, Tomas J. Acosta, Kiyoshi Okuda

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

To investigate the possible role of the vasculature in the local regulation of corpus luteum (CL) function, we determined the densities of capillaries and large blood vessels in the center of the bovine CL during the estrous cycle and following prostaglandin (PG) F2α-induced luteolysis. The CLs at the early (Days 2-3 post-ovulation), developing (Days 5-7), mid (Days 8-12), late (Days 15-17) and regressed (Days 19-21) stages were collected. In addition, the CLs were collected by transvaginal ovariectomy from 12 cows (Day 10 after ovulation), i.e., non-treated (n=3, 0 h, control), at 0.5 (n=3), 2 (n=3) and 12 h (n=3) after injection of a luteolytic dose of PGF2α. Immunohistochemical staining with von Willebrand Factor (specific for endothelial cells that are found in both types of blood vessels) revealed that the density of the luteal blood vessels was significantly higher at the developing and late luteal stages (P

Original languageEnglish
Pages (from-to)512-517
Number of pages6
JournalJournal of Reproduction and Development
Volume55
Issue number5
DOIs
Publication statusPublished - 2009

Fingerprint

luteolysis
corpus luteum
estrous cycle
prostaglandins
blood vessels
cattle
ovulation
blood coagulation factors
ovariectomy
endothelial cells
injection
cows
dosage

Keywords

  • Blood vessel
  • Bovine
  • Corpus luteum
  • Luteal endothelial cell
  • Smooth muscle layer

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Changes in the vasculature of bovine corpus luteum during the estrous cycle and prostaglandin F2α-induced luteolysis. / Hojo, Takuo; Al-Zi'abi, Mohamad O.; Skarzynski, Dariusz J.; Acosta, Tomas J.; Okuda, Kiyoshi.

In: Journal of Reproduction and Development, Vol. 55, No. 5, 2009, p. 512-517.

Research output: Contribution to journalArticle

Hojo, Takuo ; Al-Zi'abi, Mohamad O. ; Skarzynski, Dariusz J. ; Acosta, Tomas J. ; Okuda, Kiyoshi. / Changes in the vasculature of bovine corpus luteum during the estrous cycle and prostaglandin F2α-induced luteolysis. In: Journal of Reproduction and Development. 2009 ; Vol. 55, No. 5. pp. 512-517.
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