Abstract
Low temperature-resistant pea seedlings (Pisum sativum cv. Alaska), which had been kept at 2°C in darkness, during which time growth had been almost totally suppressed, were either transferred to 25°C (warmed sample) or kept at 2°C (chilled sample) for 1 day. Rapid growth occurred at 25°C. The number of nuclei in the shoot tips of the warmed sample was twice that in the chilled sample.Nuclei prepared from the warmed and chilled samples were digested by micrococcal nuclease at the same rate and appeared to have nucleosomal repeats of similar length, namely, approximately 150 base pairs, and they generated similar patterns in terms of ladders of multimers of the basic fragment.However, significant differences in terms of chromatin structure were observed when the chromatins isolated from the warmed and chilled samples were compared. The digestibility by DNase II was apparently greater in the case of chromatin prepared from the warmed sample. The analysis of the melting curves of the chromatins revealed three phases. The Tm of each phase was higher in the case of chromatin prepared from the chilled sample. Furthermore, an analysis by sucrose density gradient centrifugation revealed that the chromatin prepared from the chilled sample migrated to a region of slightly higher density in the gradient than the chromatin from the warmed sample. Taken together, these results suggest that the structure of the chromatin prepared from the chilled sample may be more compact than that of the chromatin prepared from the warmed sample.
| Original language | English |
|---|---|
| Pages (from-to) | 919-926 |
| Number of pages | 8 |
| Journal | Plant and Cell Physiology |
| Volume | 34 |
| Issue number | 6 |
| Publication status | Published - Sep 1993 |
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Keywords
- Chromatin structure
- Growth condition
- Pea shoots
- Temperature shift
ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Ecology
- Cell Biology
- Physiology
- Plant Science
Cite this
Changes in the structure of chromatin in pea shoots upon a shift in growth temperature from 2°C to 25°C. / Matsumoto, Hideaki; Yamamoto, Yoko; Kasai, Minobu; Kahl, Günter.
In: Plant and Cell Physiology, Vol. 34, No. 6, 09.1993, p. 919-926.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Changes in the structure of chromatin in pea shoots upon a shift in growth temperature from 2°C to 25°C
AU - Matsumoto, Hideaki
AU - Yamamoto, Yoko
AU - Kasai, Minobu
AU - Kahl, Günter
PY - 1993/9
Y1 - 1993/9
N2 - Low temperature-resistant pea seedlings (Pisum sativum cv. Alaska), which had been kept at 2°C in darkness, during which time growth had been almost totally suppressed, were either transferred to 25°C (warmed sample) or kept at 2°C (chilled sample) for 1 day. Rapid growth occurred at 25°C. The number of nuclei in the shoot tips of the warmed sample was twice that in the chilled sample.Nuclei prepared from the warmed and chilled samples were digested by micrococcal nuclease at the same rate and appeared to have nucleosomal repeats of similar length, namely, approximately 150 base pairs, and they generated similar patterns in terms of ladders of multimers of the basic fragment.However, significant differences in terms of chromatin structure were observed when the chromatins isolated from the warmed and chilled samples were compared. The digestibility by DNase II was apparently greater in the case of chromatin prepared from the warmed sample. The analysis of the melting curves of the chromatins revealed three phases. The Tm of each phase was higher in the case of chromatin prepared from the chilled sample. Furthermore, an analysis by sucrose density gradient centrifugation revealed that the chromatin prepared from the chilled sample migrated to a region of slightly higher density in the gradient than the chromatin from the warmed sample. Taken together, these results suggest that the structure of the chromatin prepared from the chilled sample may be more compact than that of the chromatin prepared from the warmed sample.
AB - Low temperature-resistant pea seedlings (Pisum sativum cv. Alaska), which had been kept at 2°C in darkness, during which time growth had been almost totally suppressed, were either transferred to 25°C (warmed sample) or kept at 2°C (chilled sample) for 1 day. Rapid growth occurred at 25°C. The number of nuclei in the shoot tips of the warmed sample was twice that in the chilled sample.Nuclei prepared from the warmed and chilled samples were digested by micrococcal nuclease at the same rate and appeared to have nucleosomal repeats of similar length, namely, approximately 150 base pairs, and they generated similar patterns in terms of ladders of multimers of the basic fragment.However, significant differences in terms of chromatin structure were observed when the chromatins isolated from the warmed and chilled samples were compared. The digestibility by DNase II was apparently greater in the case of chromatin prepared from the warmed sample. The analysis of the melting curves of the chromatins revealed three phases. The Tm of each phase was higher in the case of chromatin prepared from the chilled sample. Furthermore, an analysis by sucrose density gradient centrifugation revealed that the chromatin prepared from the chilled sample migrated to a region of slightly higher density in the gradient than the chromatin from the warmed sample. Taken together, these results suggest that the structure of the chromatin prepared from the chilled sample may be more compact than that of the chromatin prepared from the warmed sample.
KW - Chromatin structure
KW - Growth condition
KW - Pea shoots
KW - Temperature shift
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UR - http://www.scopus.com/inward/citedby.url?scp=77957184383&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:77957184383
VL - 34
SP - 919
EP - 926
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 6
ER -