Changes in the levels of progesterone receptor mRNA and protein in the bovine corpus luteum during the estrous cycle

Ryosuke Sakumoto, Margarete Vermehren, Rebecca A.M. Kenngott, Kiyoshi Okuda, Fred Sinowatz

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Progesterone (P4) is synthesized in the luteal cells of many species. The objective of the present study was to determine the expression pattern of P4 receptor (PR) mRNA and the distribution of PR protein in the bovine corpus luteum (CL) during the estrous cycle. The gene expression of PR in the bovine CL throughout the estrous cycle was determined by real-time PCR analysis, and the PR protein expression was evaluated by immunohistochemistry. Messenger RNA of PR was clearly expressed in the CL throughout the estrous cycle. The level of PR mRNA in the CL was highest at the early stage of the estrous cycle and was higher at the mid and late stages than at the regressed stage (P<0.01). In regard to the distribution of PR, the protein was expressed in both small and large luteal cells and in vascular endothelial cells throughout the estrous cycle. These results suggest that P4 has a role in regulating luteal and endothelial cell function in the bovine CL, especially at the early luteal stage as an autocrine/paracrine regulator.

Original languageEnglish
Pages (from-to)219-222
Number of pages4
JournalJournal of Reproduction and Development
Volume56
Issue number2
DOIs
Publication statusPublished - Apr 1 2010

Keywords

  • Corpus luteum
  • Cows
  • Immunohistochemistry
  • Progesterone
  • Receptor

ASJC Scopus subject areas

  • Animal Science and Zoology

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