Changes in the levels of progesterone receptor mRNA and protein in the bovine corpus luteum during the estrous cycle

Ryosuke Sakumoto, Margarete Vermehren, Rebecca A M Kenngott, Kiyoshi Okuda, Fred Sinowatz

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Progesterone (P4) is synthesized in the luteal cells of many species. The objective of the present study was to determine the expression pattern of P4 receptor (PR) mRNA and the distribution of PR protein in the bovine corpus luteum (CL) during the estrous cycle. The gene expression of PR in the bovine CL throughout the estrous cycle was determined by real-time PCR analysis, and the PR protein expression was evaluated by immunohistochemistry. Messenger RNA of PR was clearly expressed in the CL throughout the estrous cycle. The level of PR mRNA in the CL was highest at the early stage of the estrous cycle and was higher at the mid and late stages than at the regressed stage (P

Original languageEnglish
Pages (from-to)219-222
Number of pages4
JournalJournal of Reproduction and Development
Volume56
Issue number2
DOIs
Publication statusPublished - Apr 2010

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corpus luteum
estrous cycle
receptors
cattle
proteins
luteal cells
messenger RNA
immunohistochemistry
progesterone receptors
progesterone
quantitative polymerase chain reaction
protein synthesis
gene expression

Keywords

  • Corpus luteum
  • Cows
  • Immunohistochemistry
  • Progesterone
  • Receptor

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Changes in the levels of progesterone receptor mRNA and protein in the bovine corpus luteum during the estrous cycle. / Sakumoto, Ryosuke; Vermehren, Margarete; Kenngott, Rebecca A M; Okuda, Kiyoshi; Sinowatz, Fred.

In: Journal of Reproduction and Development, Vol. 56, No. 2, 04.2010, p. 219-222.

Research output: Contribution to journalArticle

Sakumoto, Ryosuke ; Vermehren, Margarete ; Kenngott, Rebecca A M ; Okuda, Kiyoshi ; Sinowatz, Fred. / Changes in the levels of progesterone receptor mRNA and protein in the bovine corpus luteum during the estrous cycle. In: Journal of Reproduction and Development. 2010 ; Vol. 56, No. 2. pp. 219-222.
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