Changes in the expression of novel Cdk5 activator messenger RNA (p39(nck5ai) mRNA) during rat brain development

Xiao Hui Cai, Kazuhito Tomizawa, Damu Tang, Yun Fei Lu, Akiyoshi Moriwaki, Masaaki Tokuda, Shunichiro Nagahata, Osamu Hatase, Hideki Matsui

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We previously reported that a neuron-specific Cdk5 activator, p35(nck5a), was most prominent in the newborn rat brain. In the adult brain, the expression decreased in most regions except hippocampus and primary olfactory cortex. A novel neuron-specific Cdk5 activator, p39(nck5ai), has been recently cloned. To clarify whether two activators were differentially distributed throughout brain development, in this study, we examined the spatial and temporal expression of p39(nck5ai) in the development rat brain. Northern blot analysis showed that p39(nck5ai) expression was low in 15-day old fetuses and newborn, and was most prominent in the 1-3 week-old rat brains. In the adult rat brain, expression declined to the same level as in newborn rat brain. In situ hybridization showed that p39(nck5ai) mRNA was weakly expressed in all neurons of all regions in the newborn rat brain and the transcriptional level was highest in all regions in the 3 week-old rat brain. In the adult, expression was decreased in most neurons except Purkinje and granule cells in the cerebellum which retained high levels. These results suggest that p35(nck5a) and p39(nck5ai) may have different functional roles in distinct brain regions during different states of the rat brain development.

Original languageEnglish
Pages (from-to)355-360
Number of pages6
JournalNeuroscience Research
Volume28
Issue number4
DOIs
Publication statusPublished - Aug 1997

Fingerprint

Neurons
Messenger RNA
Brain
Cdk5 activator p39
Purkinje Cells
Northern Blotting
Cerebellum
In Situ Hybridization
Hippocampus
Fetus

Keywords

  • Cdk5
  • Cerebellum
  • Cyclin-dependent kinase
  • Development
  • In situ hybridization
  • Neuron
  • p39(nck5ai)

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Changes in the expression of novel Cdk5 activator messenger RNA (p39(nck5ai) mRNA) during rat brain development. / Cai, Xiao Hui; Tomizawa, Kazuhito; Tang, Damu; Lu, Yun Fei; Moriwaki, Akiyoshi; Tokuda, Masaaki; Nagahata, Shunichiro; Hatase, Osamu; Matsui, Hideki.

In: Neuroscience Research, Vol. 28, No. 4, 08.1997, p. 355-360.

Research output: Contribution to journalArticle

Cai, XH, Tomizawa, K, Tang, D, Lu, YF, Moriwaki, A, Tokuda, M, Nagahata, S, Hatase, O & Matsui, H 1997, 'Changes in the expression of novel Cdk5 activator messenger RNA (p39(nck5ai) mRNA) during rat brain development', Neuroscience Research, vol. 28, no. 4, pp. 355-360. https://doi.org/10.1016/S0168-0102(97)00063-1
Cai, Xiao Hui ; Tomizawa, Kazuhito ; Tang, Damu ; Lu, Yun Fei ; Moriwaki, Akiyoshi ; Tokuda, Masaaki ; Nagahata, Shunichiro ; Hatase, Osamu ; Matsui, Hideki. / Changes in the expression of novel Cdk5 activator messenger RNA (p39(nck5ai) mRNA) during rat brain development. In: Neuroscience Research. 1997 ; Vol. 28, No. 4. pp. 355-360.
@article{647a3cd9048e42ceb442f6a14d5b70b7,
title = "Changes in the expression of novel Cdk5 activator messenger RNA (p39(nck5ai) mRNA) during rat brain development",
abstract = "We previously reported that a neuron-specific Cdk5 activator, p35(nck5a), was most prominent in the newborn rat brain. In the adult brain, the expression decreased in most regions except hippocampus and primary olfactory cortex. A novel neuron-specific Cdk5 activator, p39(nck5ai), has been recently cloned. To clarify whether two activators were differentially distributed throughout brain development, in this study, we examined the spatial and temporal expression of p39(nck5ai) in the development rat brain. Northern blot analysis showed that p39(nck5ai) expression was low in 15-day old fetuses and newborn, and was most prominent in the 1-3 week-old rat brains. In the adult rat brain, expression declined to the same level as in newborn rat brain. In situ hybridization showed that p39(nck5ai) mRNA was weakly expressed in all neurons of all regions in the newborn rat brain and the transcriptional level was highest in all regions in the 3 week-old rat brain. In the adult, expression was decreased in most neurons except Purkinje and granule cells in the cerebellum which retained high levels. These results suggest that p35(nck5a) and p39(nck5ai) may have different functional roles in distinct brain regions during different states of the rat brain development.",
keywords = "Cdk5, Cerebellum, Cyclin-dependent kinase, Development, In situ hybridization, Neuron, p39(nck5ai)",
author = "Cai, {Xiao Hui} and Kazuhito Tomizawa and Damu Tang and Lu, {Yun Fei} and Akiyoshi Moriwaki and Masaaki Tokuda and Shunichiro Nagahata and Osamu Hatase and Hideki Matsui",
year = "1997",
month = "8",
doi = "10.1016/S0168-0102(97)00063-1",
language = "English",
volume = "28",
pages = "355--360",
journal = "Neuroscience Research",
issn = "0168-0102",
publisher = "Elsevier Ireland Ltd",
number = "4",

}

TY - JOUR

T1 - Changes in the expression of novel Cdk5 activator messenger RNA (p39(nck5ai) mRNA) during rat brain development

AU - Cai, Xiao Hui

AU - Tomizawa, Kazuhito

AU - Tang, Damu

AU - Lu, Yun Fei

AU - Moriwaki, Akiyoshi

AU - Tokuda, Masaaki

AU - Nagahata, Shunichiro

AU - Hatase, Osamu

AU - Matsui, Hideki

PY - 1997/8

Y1 - 1997/8

N2 - We previously reported that a neuron-specific Cdk5 activator, p35(nck5a), was most prominent in the newborn rat brain. In the adult brain, the expression decreased in most regions except hippocampus and primary olfactory cortex. A novel neuron-specific Cdk5 activator, p39(nck5ai), has been recently cloned. To clarify whether two activators were differentially distributed throughout brain development, in this study, we examined the spatial and temporal expression of p39(nck5ai) in the development rat brain. Northern blot analysis showed that p39(nck5ai) expression was low in 15-day old fetuses and newborn, and was most prominent in the 1-3 week-old rat brains. In the adult rat brain, expression declined to the same level as in newborn rat brain. In situ hybridization showed that p39(nck5ai) mRNA was weakly expressed in all neurons of all regions in the newborn rat brain and the transcriptional level was highest in all regions in the 3 week-old rat brain. In the adult, expression was decreased in most neurons except Purkinje and granule cells in the cerebellum which retained high levels. These results suggest that p35(nck5a) and p39(nck5ai) may have different functional roles in distinct brain regions during different states of the rat brain development.

AB - We previously reported that a neuron-specific Cdk5 activator, p35(nck5a), was most prominent in the newborn rat brain. In the adult brain, the expression decreased in most regions except hippocampus and primary olfactory cortex. A novel neuron-specific Cdk5 activator, p39(nck5ai), has been recently cloned. To clarify whether two activators were differentially distributed throughout brain development, in this study, we examined the spatial and temporal expression of p39(nck5ai) in the development rat brain. Northern blot analysis showed that p39(nck5ai) expression was low in 15-day old fetuses and newborn, and was most prominent in the 1-3 week-old rat brains. In the adult rat brain, expression declined to the same level as in newborn rat brain. In situ hybridization showed that p39(nck5ai) mRNA was weakly expressed in all neurons of all regions in the newborn rat brain and the transcriptional level was highest in all regions in the 3 week-old rat brain. In the adult, expression was decreased in most neurons except Purkinje and granule cells in the cerebellum which retained high levels. These results suggest that p35(nck5a) and p39(nck5ai) may have different functional roles in distinct brain regions during different states of the rat brain development.

KW - Cdk5

KW - Cerebellum

KW - Cyclin-dependent kinase

KW - Development

KW - In situ hybridization

KW - Neuron

KW - p39(nck5ai)

UR - http://www.scopus.com/inward/record.url?scp=0030875972&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030875972&partnerID=8YFLogxK

U2 - 10.1016/S0168-0102(97)00063-1

DO - 10.1016/S0168-0102(97)00063-1

M3 - Article

C2 - 9274831

AN - SCOPUS:0030875972

VL - 28

SP - 355

EP - 360

JO - Neuroscience Research

JF - Neuroscience Research

SN - 0168-0102

IS - 4

ER -