TY - JOUR
T1 - Changes in parathyroid hormone-related peptide receptor binding in the shell gland of laying hens (Gallus domesticus) during the oviposition cycle
AU - Ieda, Teruko
AU - Takahashi, Tetsuya
AU - Saito, Noboru
AU - Yasuoka, Tadashi
AU - Kawashima, Mitsuo
AU - Shimada, Kiyoshi
PY - 2000/2
Y1 - 2000/2
N2 - In this study, parathyroid hormone (PTH) receptor binding was examined in the membrane fraction of the endometrium of the shell gland of laying and nonlaying hens by the use of 125I-PTH-related peptide (PTHrP) binding assays. Specific 125I-PTHrP binding was found in the endometrium of the shell gland and increased during the first 1 h of incubation and then reached a plateau. Specific 125I-PTHrP binding increased in line with an increase in protein concentration from 10 to 80 μg. The membrane fraction had specific binding to 125I-PTHrP. The Scatchard plot analysis revealed a linear profile that indicated the presence of one type of receptor. The equilibrium dissociation constant (K(d)) and the maximum binding capacity (B(max)) in the shell gland decreased 19 h before oviposition in egg-laying hens and remained low for about 20 h during the period of calcification. In contrast, the K(d) and the B(max) in nonlaying hens were constant throughout a 24-h period. These results suggest that PTH acts on the endometrium of the shell gland during the eggshell calcification. (C) 2000 Academic Press.
AB - In this study, parathyroid hormone (PTH) receptor binding was examined in the membrane fraction of the endometrium of the shell gland of laying and nonlaying hens by the use of 125I-PTH-related peptide (PTHrP) binding assays. Specific 125I-PTHrP binding was found in the endometrium of the shell gland and increased during the first 1 h of incubation and then reached a plateau. Specific 125I-PTHrP binding increased in line with an increase in protein concentration from 10 to 80 μg. The membrane fraction had specific binding to 125I-PTHrP. The Scatchard plot analysis revealed a linear profile that indicated the presence of one type of receptor. The equilibrium dissociation constant (K(d)) and the maximum binding capacity (B(max)) in the shell gland decreased 19 h before oviposition in egg-laying hens and remained low for about 20 h during the period of calcification. In contrast, the K(d) and the B(max) in nonlaying hens were constant throughout a 24-h period. These results suggest that PTH acts on the endometrium of the shell gland during the eggshell calcification. (C) 2000 Academic Press.
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U2 - 10.1006/gcen.1999.7395
DO - 10.1006/gcen.1999.7395
M3 - Article
C2 - 10642440
AN - SCOPUS:0033981378
VL - 117
SP - 182
EP - 188
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
SN - 0016-6480
IS - 2
ER -