Changes in Nitric Oxide Production Levels and Expression of Nitric Oxide Synthase Isoforms in the Rat Uterus during pregnancy

Takehito Suzuki, Chiemi Mori, Hiroshi Yoshikawa, Yoko Miyazaki, Norio Kansaku, Kazuaki Tanaka, Hidetoshi Morita, Tatsuya Takizawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We clarified nitric oxide (NO) production in the rat uterus by electron paramagnetic resonance spetroscopy and with Fe-N-(dithiocarboxy) sarcosine complex (an NO-trapping reagent). We examined changes in NO production in the whole uterus, decidua, and myometrium (gestational days 13.5-21.5). The expression of nitric oxide synthase (NOS) isoforms was also examined by quantitative reverse transcription-poly- merase chain reaction. The uterine NO levels were low on day 13.5, peaked on day 17.5, and thereafter decreased significantly. The NO production levels in the decidua and myometrium were the same on day 13.5, but the levels in the decidua were 2- to 4-fold higher than those in the myometrium from day 15.5 onwards. The NOS-2 mRNA expression pattern correlated well with changes in the NO levels in the decidua, whereas the NOS-3 mRNA was expressed constantly during gestation. Thus NOS-2-generated NO in the decidua contributed significantly to uterine NO levels.

Original languageEnglish
Pages (from-to)2163-2166
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number10
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Nitric Oxide Synthase
Uterus
Rats
Nitric Oxide
Protein Isoforms
Decidua
Pregnancy
Myometrium
Messenger RNA
Polymerase chain reaction
Electron Spin Resonance Spectroscopy
Transcription
Reverse Transcription
Paramagnetic resonance
Polymerase Chain Reaction

Keywords

  • Electron paramagnetic resonance (EPR)
  • Nitric oxide
  • Uterus

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Changes in Nitric Oxide Production Levels and Expression of Nitric Oxide Synthase Isoforms in the Rat Uterus during pregnancy. / Suzuki, Takehito; Mori, Chiemi; Yoshikawa, Hiroshi; Miyazaki, Yoko; Kansaku, Norio; Tanaka, Kazuaki; Morita, Hidetoshi; Takizawa, Tatsuya.

In: Bioscience, Biotechnology and Biochemistry, Vol. 73, No. 10, 2009, p. 2163-2166.

Research output: Contribution to journalArticle

Suzuki, Takehito ; Mori, Chiemi ; Yoshikawa, Hiroshi ; Miyazaki, Yoko ; Kansaku, Norio ; Tanaka, Kazuaki ; Morita, Hidetoshi ; Takizawa, Tatsuya. / Changes in Nitric Oxide Production Levels and Expression of Nitric Oxide Synthase Isoforms in the Rat Uterus during pregnancy. In: Bioscience, Biotechnology and Biochemistry. 2009 ; Vol. 73, No. 10. pp. 2163-2166.
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