Changes in mRNA expression of MMP-2 in the Müllerian duct of chicken embryo

Yonju Ha, Akira Tsukada, Noboru Saito, Kiyoshi Shimada

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Although asymmetric development of the ovary and the oviduct is a unique characteristic in birds, the mechanism of asymmetric development still remains unclear. Recently, degradation of extracellular matrix has been suggested as an important factor related to the regression of the Müllerian duct in mammals. The present study was conducted to examine a possible role of metalloproteinase-2 (MMP-2) in the regression of the right Müllerian duct in the developing chicken embryo. Morphological changes in the Müllerian ducts were studied on day 15 of incubation and mRNA expresseion of MMP-2 was studied on days 12, 15, and 18 of incubation. Morphological observation demonstrated the disappearance of basement membrane in the right Müllerian duct which undergoes the regression. RT-PCR analysis showed that MMP-2 mRNA expression of the right Müllerian duct increased on days 15 and 18 of incubation coincidently with the time of regression. In the right Müllerian duct, regression was prevented by diethylstilbestrol treatment on day 4 of incubation and a coincident decrease in MMP-2 expression was observed when compared to the control group. These results suggest that MMP-2 may be involved in the regression of the right Müllerian duct in the female embryos of the chicken.

Original languageEnglish
Pages (from-to)131-136
Number of pages6
JournalGeneral and Comparative Endocrinology
Volume139
Issue number2
DOIs
Publication statusPublished - Nov 2004
Externally publishedYes

Keywords

  • Chicken
  • Diethylstilbestrol
  • Embryo
  • Extracellular matrix
  • Matrix metalloproteinase-2
  • M̈llerian duct

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

Fingerprint Dive into the research topics of 'Changes in mRNA expression of MMP-2 in the Müllerian duct of chicken embryo'. Together they form a unique fingerprint.

  • Cite this