Changes in lipid peroxide and antioxidant enzyme activities in corpora lutea during pseudopregnancy in rats

K. Shimamura, N. Sukino, Y. Yoshida, Y. Nakamura, Keiki Ogino, H. Kato

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

This study investigated the involvement of lipid peroxidation and antioxidant enzymes in the regulation of luteal function in pseudopregnant rats. The activities of superoxide dismutase (SOD), a specific scavenger of superoxide radicals, and glutathione peroxidase, a scavenger of hydrogen peroxide, and lipid peroxide concentrations were measured in the corpus luteum on days 1, 3, 5, 7, 9, 11 and 13 of pseudopregnancy. The activity of SOD in the corpus luteum gradually increased until day 9 of pseudopregnancy and decreased thereafter, in a similar manner to serum progesterone concentration. Glutathione peroxidase activity significantly increased from day 1 to day 3 and remained high until day 11 of pseudopregnancy. The concentrations of lipid peroxides in the corpus luteum increased from day 3 to day 13 of pseudopregnancy. The involvement of prostaglandin F(2α) (PGF(2α)) in the production of lipid peroxides in regression of the corpus luteum was investigated by administering PGF(2α) (3 mg kg-1, s.c.) or saline on days 7, 9 and 12 of pseudopregnancy. Each group of rats was autopsied 2 h later, and SOD activity, glutathione peroxidase activity and the concentration of lipid peroxides in the corpus luteum were determined. PGF(2α) significantly increased lipid peroxide concentrations in the corpus luteum on days 7, 9 and 12 of pseudopregnancy (approximately twofold increases on days 7 and 9, and a fivefold increase on day 12, compared with the control that received saline). The activity of SOD in the corpus luteum was significantly increased by PGF(2α) on days 7 and 9, but not on day 12, of pseudopregnancy. PGF(2α) did not cause any significant changes in glutathione peroxidase activity in the corpus luteum on days 7, 9 and 12 of pseudopregnancy. It is concluded that lipid peroxides play an important role in regulating luteal function in pseudopregnant rats.

Original languageEnglish
Pages (from-to)253-257
Number of pages5
JournalJournal of Reproduction and Fertility
Volume105
Issue number2
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Pseudopregnancy
Lipid Peroxides
Corpus Luteum
Antioxidants
Prostaglandins F
Enzymes
Glutathione Peroxidase
Superoxide Dismutase
Luteolysis
Superoxides
Hydrogen Peroxide
Lipid Peroxidation
Progesterone

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Embryology
  • Developmental Biology
  • Molecular Biology
  • Physiology

Cite this

Changes in lipid peroxide and antioxidant enzyme activities in corpora lutea during pseudopregnancy in rats. / Shimamura, K.; Sukino, N.; Yoshida, Y.; Nakamura, Y.; Ogino, Keiki; Kato, H.

In: Journal of Reproduction and Fertility, Vol. 105, No. 2, 1995, p. 253-257.

Research output: Contribution to journalArticle

Shimamura, K. ; Sukino, N. ; Yoshida, Y. ; Nakamura, Y. ; Ogino, Keiki ; Kato, H. / Changes in lipid peroxide and antioxidant enzyme activities in corpora lutea during pseudopregnancy in rats. In: Journal of Reproduction and Fertility. 1995 ; Vol. 105, No. 2. pp. 253-257.
@article{ba16f124f8e74086bff339e27ad687dd,
title = "Changes in lipid peroxide and antioxidant enzyme activities in corpora lutea during pseudopregnancy in rats",
abstract = "This study investigated the involvement of lipid peroxidation and antioxidant enzymes in the regulation of luteal function in pseudopregnant rats. The activities of superoxide dismutase (SOD), a specific scavenger of superoxide radicals, and glutathione peroxidase, a scavenger of hydrogen peroxide, and lipid peroxide concentrations were measured in the corpus luteum on days 1, 3, 5, 7, 9, 11 and 13 of pseudopregnancy. The activity of SOD in the corpus luteum gradually increased until day 9 of pseudopregnancy and decreased thereafter, in a similar manner to serum progesterone concentration. Glutathione peroxidase activity significantly increased from day 1 to day 3 and remained high until day 11 of pseudopregnancy. The concentrations of lipid peroxides in the corpus luteum increased from day 3 to day 13 of pseudopregnancy. The involvement of prostaglandin F(2α) (PGF(2α)) in the production of lipid peroxides in regression of the corpus luteum was investigated by administering PGF(2α) (3 mg kg-1, s.c.) or saline on days 7, 9 and 12 of pseudopregnancy. Each group of rats was autopsied 2 h later, and SOD activity, glutathione peroxidase activity and the concentration of lipid peroxides in the corpus luteum were determined. PGF(2α) significantly increased lipid peroxide concentrations in the corpus luteum on days 7, 9 and 12 of pseudopregnancy (approximately twofold increases on days 7 and 9, and a fivefold increase on day 12, compared with the control that received saline). The activity of SOD in the corpus luteum was significantly increased by PGF(2α) on days 7 and 9, but not on day 12, of pseudopregnancy. PGF(2α) did not cause any significant changes in glutathione peroxidase activity in the corpus luteum on days 7, 9 and 12 of pseudopregnancy. It is concluded that lipid peroxides play an important role in regulating luteal function in pseudopregnant rats.",
author = "K. Shimamura and N. Sukino and Y. Yoshida and Y. Nakamura and Keiki Ogino and H. Kato",
year = "1995",
language = "English",
volume = "105",
pages = "253--257",
journal = "Journal of Reproduction and Fertility",
issn = "0022-4251",
publisher = "Society for Reproduction and Fertility",
number = "2",

}

TY - JOUR

T1 - Changes in lipid peroxide and antioxidant enzyme activities in corpora lutea during pseudopregnancy in rats

AU - Shimamura, K.

AU - Sukino, N.

AU - Yoshida, Y.

AU - Nakamura, Y.

AU - Ogino, Keiki

AU - Kato, H.

PY - 1995

Y1 - 1995

N2 - This study investigated the involvement of lipid peroxidation and antioxidant enzymes in the regulation of luteal function in pseudopregnant rats. The activities of superoxide dismutase (SOD), a specific scavenger of superoxide radicals, and glutathione peroxidase, a scavenger of hydrogen peroxide, and lipid peroxide concentrations were measured in the corpus luteum on days 1, 3, 5, 7, 9, 11 and 13 of pseudopregnancy. The activity of SOD in the corpus luteum gradually increased until day 9 of pseudopregnancy and decreased thereafter, in a similar manner to serum progesterone concentration. Glutathione peroxidase activity significantly increased from day 1 to day 3 and remained high until day 11 of pseudopregnancy. The concentrations of lipid peroxides in the corpus luteum increased from day 3 to day 13 of pseudopregnancy. The involvement of prostaglandin F(2α) (PGF(2α)) in the production of lipid peroxides in regression of the corpus luteum was investigated by administering PGF(2α) (3 mg kg-1, s.c.) or saline on days 7, 9 and 12 of pseudopregnancy. Each group of rats was autopsied 2 h later, and SOD activity, glutathione peroxidase activity and the concentration of lipid peroxides in the corpus luteum were determined. PGF(2α) significantly increased lipid peroxide concentrations in the corpus luteum on days 7, 9 and 12 of pseudopregnancy (approximately twofold increases on days 7 and 9, and a fivefold increase on day 12, compared with the control that received saline). The activity of SOD in the corpus luteum was significantly increased by PGF(2α) on days 7 and 9, but not on day 12, of pseudopregnancy. PGF(2α) did not cause any significant changes in glutathione peroxidase activity in the corpus luteum on days 7, 9 and 12 of pseudopregnancy. It is concluded that lipid peroxides play an important role in regulating luteal function in pseudopregnant rats.

AB - This study investigated the involvement of lipid peroxidation and antioxidant enzymes in the regulation of luteal function in pseudopregnant rats. The activities of superoxide dismutase (SOD), a specific scavenger of superoxide radicals, and glutathione peroxidase, a scavenger of hydrogen peroxide, and lipid peroxide concentrations were measured in the corpus luteum on days 1, 3, 5, 7, 9, 11 and 13 of pseudopregnancy. The activity of SOD in the corpus luteum gradually increased until day 9 of pseudopregnancy and decreased thereafter, in a similar manner to serum progesterone concentration. Glutathione peroxidase activity significantly increased from day 1 to day 3 and remained high until day 11 of pseudopregnancy. The concentrations of lipid peroxides in the corpus luteum increased from day 3 to day 13 of pseudopregnancy. The involvement of prostaglandin F(2α) (PGF(2α)) in the production of lipid peroxides in regression of the corpus luteum was investigated by administering PGF(2α) (3 mg kg-1, s.c.) or saline on days 7, 9 and 12 of pseudopregnancy. Each group of rats was autopsied 2 h later, and SOD activity, glutathione peroxidase activity and the concentration of lipid peroxides in the corpus luteum were determined. PGF(2α) significantly increased lipid peroxide concentrations in the corpus luteum on days 7, 9 and 12 of pseudopregnancy (approximately twofold increases on days 7 and 9, and a fivefold increase on day 12, compared with the control that received saline). The activity of SOD in the corpus luteum was significantly increased by PGF(2α) on days 7 and 9, but not on day 12, of pseudopregnancy. PGF(2α) did not cause any significant changes in glutathione peroxidase activity in the corpus luteum on days 7, 9 and 12 of pseudopregnancy. It is concluded that lipid peroxides play an important role in regulating luteal function in pseudopregnant rats.

UR - http://www.scopus.com/inward/record.url?scp=0029620833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029620833&partnerID=8YFLogxK

M3 - Article

VL - 105

SP - 253

EP - 257

JO - Journal of Reproduction and Fertility

JF - Journal of Reproduction and Fertility

SN - 0022-4251

IS - 2

ER -