TY - JOUR
T1 - Changes in lipid peroxidation, Cu/Zn-superoxide dismutase and its mRNA following an intracerebroventricular injection of 6-hydroxydopamine in mice
AU - Ogawa, Norio
AU - Asanuma, Masato
AU - Kondo, Yoichi
AU - Hirata, Hiroshi
AU - Nishibayashi, Sakiko
AU - Mori, Akitane
N1 - Funding Information:
This work was supported in part by Grant-in-Aid for Scientific Research from the Japanese Ministry of Education, Science and Culture, and the Grants for Research Projects on Aging and Health and for the Research Committee of CNS Degenerative Diseases from the Japanese Ministry of Health and Welfare. We thank K.H. Ono for her excellent technical assistance.
PY - 1994/5/23
Y1 - 1994/5/23
N2 - A single i.c.v. injection of 6-hydroxydopamine (6-OHDA) in mice resulted in a biphasic increase in lipid peroxidation as assayed by the level of thiobarbituric acid-reacting substances (TBARS). An increase in Cu/Zn-superoxide dismutase (SOD) activity was temporally related with the first peak of TBARS but remained unchanged during the second TBARS peak. This suggests that a free radical species other than O2- may be involved in the late onset increase in TBARS. The level of Cu/Zn-SOD mRNA did not immediately reflect the change in Cu/Zn-SOD activity but rather increased gradually reaching significantly higher levels only 8 weeks after i.c.v. an injection of 6-OHDA. This increase in Cu/Zn-SOD mRNA likely occurs in response to a consumption of intrinsic SOD. Thus, short- and long-term increases in lipid peroxidation likely occur by different mechanisms and studies of both are needed to elucidate the neurodegenerative process.
AB - A single i.c.v. injection of 6-hydroxydopamine (6-OHDA) in mice resulted in a biphasic increase in lipid peroxidation as assayed by the level of thiobarbituric acid-reacting substances (TBARS). An increase in Cu/Zn-superoxide dismutase (SOD) activity was temporally related with the first peak of TBARS but remained unchanged during the second TBARS peak. This suggests that a free radical species other than O2- may be involved in the late onset increase in TBARS. The level of Cu/Zn-SOD mRNA did not immediately reflect the change in Cu/Zn-SOD activity but rather increased gradually reaching significantly higher levels only 8 weeks after i.c.v. an injection of 6-OHDA. This increase in Cu/Zn-SOD mRNA likely occurs in response to a consumption of intrinsic SOD. Thus, short- and long-term increases in lipid peroxidation likely occur by different mechanisms and studies of both are needed to elucidate the neurodegenerative process.
KW - 6-Hydroxydopamine
KW - Cu/Zn-SOD mRNA
KW - Lipid peroxidation
KW - Superoxide dismutase (SOD)
KW - Thiobarbituric acid-reacting substance (TBARS)
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U2 - 10.1016/0006-8993(94)90102-3
DO - 10.1016/0006-8993(94)90102-3
M3 - Article
C2 - 8069686
AN - SCOPUS:0028214340
SN - 0006-8993
VL - 646
SP - 337
EP - 340
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 2
ER -