Changes in lipid peroxidation, Cu/Zn-superoxide dismutase and its mRNA following an intracerebroventricular injection of 6-hydroxydopamine in mice

Norio Ogawa, Masato Asanuma, Yoichi Kondo, Hiroshi Hirata, Sakiko Nishibayashi, Akitane Mori

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A single i.c.v. injection of 6-hydroxydopamine (6-OHDA) in mice resulted in a biphasic increase in lipid peroxidation as assayed by the level of thiobarbituric acid-reacting substances (TBARS). An increase in Cu/Zn-superoxide dismutase (SOD) activity was temporally related with the first peak of TBARS but remained unchanged during the second TBARS peak. This suggests that a free radical species other than O2- may be involved in the late onset increase in TBARS. The level of Cu/Zn-SOD mRNA did not immediately reflect the change in Cu/Zn-SOD activity but rather increased gradually reaching significantly higher levels only 8 weeks after i.c.v. an injection of 6-OHDA. This increase in Cu/Zn-SOD mRNA likely occurs in response to a consumption of intrinsic SOD. Thus, short- and long-term increases in lipid peroxidation likely occur by different mechanisms and studies of both are needed to elucidate the neurodegenerative process.

Original languageEnglish
Pages (from-to)337-340
Number of pages4
JournalBrain Research
Volume646
Issue number2
DOIs
Publication statusPublished - May 23 1994

Fingerprint

Oxidopamine
Lipid Peroxidation
Messenger RNA
Injections
Superoxide Dismutase
Free Radicals
thiobarbituric acid
Superoxide Dismutase-1

Keywords

  • 6-Hydroxydopamine
  • Cu/Zn-SOD mRNA
  • Lipid peroxidation
  • Superoxide dismutase (SOD)
  • Thiobarbituric acid-reacting substance (TBARS)

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Changes in lipid peroxidation, Cu/Zn-superoxide dismutase and its mRNA following an intracerebroventricular injection of 6-hydroxydopamine in mice. / Ogawa, Norio; Asanuma, Masato; Kondo, Yoichi; Hirata, Hiroshi; Nishibayashi, Sakiko; Mori, Akitane.

In: Brain Research, Vol. 646, No. 2, 23.05.1994, p. 337-340.

Research output: Contribution to journalArticle

Ogawa, Norio ; Asanuma, Masato ; Kondo, Yoichi ; Hirata, Hiroshi ; Nishibayashi, Sakiko ; Mori, Akitane. / Changes in lipid peroxidation, Cu/Zn-superoxide dismutase and its mRNA following an intracerebroventricular injection of 6-hydroxydopamine in mice. In: Brain Research. 1994 ; Vol. 646, No. 2. pp. 337-340.
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